Novel and economical purification of recombinant proteins: intein-mediated protein purification using in vivo polyhydroxybutyrate (PHB) matrix association
about
Inteins and affinity resin substitutes for protein purification and scale upTo fuse or not to fuse: what is your purpose?In vivo enzyme immobilization by use of engineered polyhydroxyalkanoate synthaseRecombinant Escherichia coli strain produces a ZZ domain displaying biopolyester granules suitable for immunoglobulin G purificationRecombinant Escherichia coli produces tailor-made biopolyester granules for applications in fluorescence activated cell sorting: functional display of the mouse interleukin-2 and myelin oligodendrocyte glycoprotein.Intein applications: from protein purification and labeling to metabolic control methods.Expression of active recombinant human tissue-type plasminogen activator by using in vivo polyhydroxybutyrate granule display.Microbial polyhydroxyalkanote synthesis repression protein PhaR as an affinity tag for recombinant protein purification.Relevant uses of surface proteins--display on self-organized biological structures.Heterologous expression of human costimulatory molecule B7-2 and construction of B7-2 immobilized polyhydroxyalkanoate nanoparticles for use as an immune activation agentElectronic structure of neighboring extein residue modulates intein C-terminal cleavage activityHigh Efficient Expression, Purification, and Functional Characterization of Native Human Epidermal Growth Factor in Escherichia coli.Structural Insights on PHA Binding Protein PhaP from Aeromonas hydrophila.Inteins, valuable genetic elements in molecular biology and biotechnology.Self-cleaving fusion tags for recombinant protein production.Cellular and organellar membrane-associated proteins in haloarchaea: perspectives on the physiological significance and biotechnological applications.Co-expression for intracellular processing in microbial protein production.Considerations for the process development of insect-derived antimicrobial peptide production.Production and purification of an untagged recombinant pneumococcal surface protein A (PspA4Pro) with high-purity and low endotoxin content.Recombinant protein production by in vivo polymer inclusion display.Phasins, Multifaceted Polyhydroxyalkanoate Granule-Associated Proteins.Purification of Antibacterial CHAPK Protein Using a Self-Cleaving Fusion Tag and Its Activity Against Methicillin-Resistant Staphylococcus aureus.Polyhydroxyalkanoate-associated phasins as phylogenetically heterogeneous, multipurpose proteins.Tobacco etch virus protease mediating cleavage of the cellulose-binding module tagged colored proteins immobilized on the regenerated amorphous cellulose.Tolerance of the Ralstonia eutropha class I polyhydroxyalkanoate synthase for translational fusions to its C terminus reveals a new mode of functional display.Engineering bacteria to manufacture functionalized polyester beads.In vivo polyester immobilized sortase for tagless protein purification.Monitoring and kinetic analysis of the molecular interactions by which a repressor protein, PhaR, binds to target DNAs and poly[(R)-3-hydroxybutyrate]Proteins from PHB granules.Binding of the major phasin, PhaP1, from Ralstonia eutropha H16 to poly(3-hydroxybutyrate) granules.Modelling of microbial polyhydroxyalkanoate surface binding protein PhaP for rational mutagenesis.Purification of target proteins from intracellular inclusions mediated by intein cleavable polyhydroxyalkanoate synthase fusions.Poly-3-hydroxybutyrate Functionalization with BioF-Tagged Recombinant Proteins.Covalent-display of an active chimeric-recombinant tissue plasminogen activator on polyhydroxybutyrate granules surface.A phasin with extra talents: a polyhydroxyalkanoate granule-associated protein has chaperone activity.Production of recombinant porcine interferon alpha using PHB-intein-mediated protein purification strategy.Inteins: Localized Distribution, Gene Regulation, and Protein Engineering for Biological Applications.
P2860
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P2860
Novel and economical purification of recombinant proteins: intein-mediated protein purification using in vivo polyhydroxybutyrate (PHB) matrix association
description
2005 nî lūn-bûn
@nan
2005 թուականի Յունիսին հրատարակուած գիտական յօդուած
@hyw
2005 թվականի հունիսին հրատարակված գիտական հոդված
@hy
2005年の論文
@ja
2005年論文
@yue
2005年論文
@zh-hant
2005年論文
@zh-hk
2005年論文
@zh-mo
2005年論文
@zh-tw
2005年论文
@wuu
name
Novel and economical purificat ...... yrate (PHB) matrix association
@ast
Novel and economical purificat ...... yrate (PHB) matrix association
@en
Novel and economical purificat ...... yrate (PHB) matrix association
@nl
type
label
Novel and economical purificat ...... yrate (PHB) matrix association
@ast
Novel and economical purificat ...... yrate (PHB) matrix association
@en
Novel and economical purificat ...... yrate (PHB) matrix association
@nl
prefLabel
Novel and economical purificat ...... yrate (PHB) matrix association
@ast
Novel and economical purificat ...... yrate (PHB) matrix association
@en
Novel and economical purificat ...... yrate (PHB) matrix association
@nl
P2093
P2860
P3181
P356
P1433
P1476
Novel and economical purificat ...... yrate (PHB) matrix association
@en
P2093
David W Wood
Mahmoud Reza Banki
Tillman U Gerngross
P2860
P304
P3181
P356
10.1110/PS.041296305
P407
P577
2005-06-01T00:00:00Z