about
Temporal expression of HIV-1 envelope proteins in baculovirus-infected insect cells: implications for glycosylation and CD4 bindingDirect DNA hybridization screening of primary yeast transformants in the construction of targeted yeast artificial chromosome (YAC) libraries.Detection of individual human chromosomes by chromosome in situ suppression hybridization using PCR-amplified bacteriophage library probes.Creation of a yeast artificial chromosome fragmentation vector based on lysine-2.Cloning of PCR-amplified total cDNA: construction of a mouse oocyte cDNA library.A novel approach to the rapid isolation and nucleotide sequencing of genomic clones.Differences in the probability of cloning specific DNA between primary and amplified libraries: theoretical considerations.A simple, effective method for the construction of subtracted cDNA libraries.Agarose entrapment method for the production of SfiI linking library for Theileria parva.Rapid DNA sequencing based upon single molecule detection.DNA sequence mapping using electron microscopy.Can the scanning tunneling microscope sequence DNA?Chromosomal in situ hybridization using yeast artificial chromosomes.Current methods for YAC clone characterization.Modification and manipulation of mammalian DNA cloned as YACs.YAC cloning of DNA embedded in an agarose matrix.Alternatives to YACs.YAC cloning: options and problems.YAC cloning of telomeres.Direct cloning of cDNA inserts from lambda gt11 phage DNA into a plasmid vector by a novel and simple method.Subtraction hybridization cDNA libraries from colon carcinoma and hepatic cancer.Detection of mutations in human DNA.PCR-SSCP: a method for detection of mutations.Localizing DNA and RNA within nuclei and chromosomes by fluorescence in situ hybridization.Optimization of subtractive hybridization in construction of subtractive cDNA libraries.Direct sequencing of terminal regions of genomic P1 clones. A general strategy for the design of sequence-tagged site markers.Large-scale isolation of human 1p36-specific P1 clones and their use for fluorescence in situ hybridization.Construction and preliminary analysis of the ICRF human P1 library.A single P1 clone bearing three genes from human chromosome 11p15.5: HRC1, HRAS1, and RNH.Three new developments in P1 cloning. Increased cloning efficiency, improved clone recovery, and a new P1 mouse library.The complete nucleotide sequences of the SacBII Kan domain of the P1 pAD10-SacBII cloning vector and three cosmid cloning vectors: pTCF, svPHEP, and LAWRIST16.Chromosomal fragile sites.Simple and robust screening of pooled yeast artificial chromosome libraries by the restriction enzyme digestion of polymerase chain reaction products.Accelerated screening of cDNA libraries using the polymerase chain reaction and Southern blotting.Screening cosmid libraries with oligonucleotides corresponding to splice-site consensus sequences.Rapid isolation of cosmid insert DNA by triple-helix-mediated affinity capture.Rescue of a single yeast artificial chromosome from a cotransformation event utilizing segregation at meiosis.Screening yeast artificial chromosome libraries with robot-aided automation.Construction and characterization of partial digest DNA libraries made from flow-sorted human chromosome 16.Relevance of empirical optimization for cross-linking DNA by using an ordinary ultraviolet-light source.
P1433
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P1433
description
journal
@en
revista científica
@es
rivista scientifica
@it
wetenschappelijk tijdschrift van Elsevier
@nl
wissenschaftliche Fachzeitschrift
@de
name
Genetic analysis, techniques and applications
@ast
Genetic analysis, techniques and applications
@en
Genetic analysis, techniques and applications
@es
Genetic analysis, techniques and applications
@it
Genetic analysis, techniques and applications
@nl
type
label
Genetic analysis, techniques and applications
@ast
Genetic analysis, techniques and applications
@en
Genetic analysis, techniques and applications
@es
Genetic analysis, techniques and applications
@it
Genetic analysis, techniques and applications
@nl
altLabel
Genet Anal Tech Appl
@en
Genet. Anal. Tech. Appl.
@en
prefLabel
Genetic analysis, techniques and applications
@ast
Genetic analysis, techniques and applications
@en
Genetic analysis, techniques and applications
@es
Genetic analysis, techniques and applications
@it
Genetic analysis, techniques and applications
@nl
P3181
P1055
P123
P1476
Genetic analysis, techniques and applications
@en