Crystallographic observation of a covalent catalytic intermediate in a beta-glycosidase
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Crystal structure of the beta-glycosidase from the hyperthermophile Thermosphaera aggregans: insights into its activity and thermostabilitySubstrate specificity in glycoside hydrolase family 10. Structural and kinetic analysis of the Streptomyces lividans xylanase 10AHigh resolution X-ray crystallography shows that ascorbate is a cofactor for myrosinase and substitutes for the function of the catalytic baseX-ray crystallographic study of xylopentaose binding to Pseudomonas fluorescens xylanase ABiochemical and structural assessment of the 1-N-azasugar GalNAc-isofagomine as a potent family 20 beta-N-acetylhexosaminidase inhibitorSubstrate specificity and subsite mobility in T. aurantiacus xylanase 10AStructural Basis for Broad Substrate Specificity in Higher Plant beta-D-Glucan GlucohydrolasesCrystal structure and snapshots along the reaction pathway of a family 51 -L-arabinofuranosidaseThe mechanisms by which family 10 glycoside hydrolases bind decorated substratesStructural and biochemical analysis of Cellvibrio japonicus xylanase 10C: how variation in substrate-binding cleft influences the catalytic profile of family GH-10 xylanasesFunctional analysis of hyperthermophilic endocellulase from Pyrococcus horikoshii by crystallographic snapshotsBiochemical properties and atomic resolution structure of a proteolytically processed β-mannanase from cellulolytic Streptomyces sp. SirexAA-ESemi-rational approach for converting a GH1 β-glycosidase into a β-transglycosidaseStructure-based investigation into the functional roles of the extended loop and substrate-recognition sites in an endo-β-1,4-D-mannanase from the Antarctic springtail, Cryptopygus antarcticusRational design of a β-glycosidase with high regiospecificity for triterpenoid tailoringInsights into transition state stabilization of the beta-1,4-glycosidase Cex by covalent intermediate accumulation in active site mutantsEpoxyalkyl glycosides of D-xylose and xylo-oligosaccharides are active-site markers of xylanases from glycoside hydrolase family 11, not from family 10Identification of essential active-site residues in the cyanogenic beta-glucosidase (linamarase) from cassava (Manihot esculenta Crantz) by site-directed mutagenesisProtein dielectric constants determined from NMR chemical shift perturbationsSynthesis and characterization of novel lacZ gene reporter molecules: detection of beta-galactosidase activity by 19F nuclear magnetic resonance of polyglycosylated fluorinated vitamin B6Differential mechanism-based labeling and unequivocal activity assignment of the two active sites of intestinal lactase/phlorizin hydrolase.Directed evolution of a glycosynthase from Agrobacterium sp. increases its catalytic activity dramatically and expands its substrate repertoire.Structure, dynamics, and specificity of endoglucanase D from Clostridium cellulovoransA family 8 glycoside hydrolase from Bacillus halodurans C-125 (BH2105) is a reducing end xylose-releasing exo-oligoxylanase.4-Deoxy-4-fluoro-β-D-glucopyranose.Co-crystals of 3-deoxy-3-fluoro-α-D-glucopyranose and 3-deoxy-3-fluoro-β-D-glucopyranose.A hydrophobic platform as a mechanistically relevant transition state stabilising factor appears to be present in the active centre of all glycoside hydrolases.Structure of the xylanase from Penicillium simplicissimumStructural determinants of the substrate specificities of xylanases from different glycoside hydrolase families.Substrate specificity in glycoside hydrolase family 10. Tyrosine 87 and leucine 314 play a pivotal role in discriminating between glucose and xylose binding in the proximal active site of Pseudomonas cellulosa xylanase 10A.Characterization of active-site aromatic residues in xylanase A from Streptomyces lividans.Key residues in subsite F play a critical role in the activity of Pseudomonas fluorescens subspecies cellulosa xylanase A against xylooligosaccharides but not against highly polymeric substrates such as xylan.Studies on identifying the catalytic role of Glu-204 in the active site of yeast invertase.Structural considerations on the use of endo-xylanases for the production of prebiotic xylooligosaccharides from biomass.Use of Fluorinated Functionality in Enzyme Inhibitor Development: Mechanistic and Analytical AdvantagesAnalysis of the dynamic properties of Bacillus circulans xylanase upon formation of a covalent glycosyl-enzyme intermediateSlow-tight binding inhibition of xylanase by an aspartic protease inhibitor: kinetic parameters and conformational changes that determine the affinity and selectivity of the bifunctional nature of the inhibitor.Inhibition of 1,4-beta-D-xylan xylanohydrolase by the specific aspartic protease inhibitor pepstatin: probing the two-step inhibition mechanism.Structure and function of a family 10 beta-xylanase chimera of Streptomyces olivaceoviridis E-86 FXYN and Cellulomonas fimi Cex.An elaboration on the syn-anti proton donor concept of glycoside hydrolases: electrostatic stabilisation of the transition state as a general strategy.
P2860
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P2860
Crystallographic observation of a covalent catalytic intermediate in a beta-glycosidase
description
1996 nî lūn-bûn
@nan
1996 թուականի Փետրուարին հրատարակուած գիտական յօդուած
@hyw
1996 թվականի փետրվարին հրատարակված գիտական հոդված
@hy
1996年の論文
@ja
1996年論文
@yue
1996年論文
@zh-hant
1996年論文
@zh-hk
1996年論文
@zh-mo
1996年論文
@zh-tw
1996年论文
@wuu
name
Crystallographic observation of a covalent catalytic intermediate in a beta-glycosidase
@ast
Crystallographic observation of a covalent catalytic intermediate in a beta-glycosidase
@en
Crystallographic observation of a covalent catalytic intermediate in a beta-glycosidase
@nl
type
label
Crystallographic observation of a covalent catalytic intermediate in a beta-glycosidase
@ast
Crystallographic observation of a covalent catalytic intermediate in a beta-glycosidase
@en
Crystallographic observation of a covalent catalytic intermediate in a beta-glycosidase
@nl
prefLabel
Crystallographic observation of a covalent catalytic intermediate in a beta-glycosidase
@ast
Crystallographic observation of a covalent catalytic intermediate in a beta-glycosidase
@en
Crystallographic observation of a covalent catalytic intermediate in a beta-glycosidase
@nl
P2093
P2860
P356
P1476
Crystallographic observation of a covalent catalytic intermediate in a beta-glycosidase
@en
P2093
P2860
P304
P356
10.1038/NSB0296-149
P577
1996-02-01T00:00:00Z