One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-Tag
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Engineering a novel multifunctional green fluorescent protein tag for a wide variety of protein researchStreptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complexIdentification of cyclophilin-40-interacting proteins reveals potential cellular function of cyclophilin-40Free Brick1 is a trimeric precursor in the assembly of a functional wave complexTwo translocating hydrophilic segments of a nascent chain span the ER membrane during multispanning protein topogenesisRemodeling of the lectin-EGF-like domain interface in P- and L-selectin increases adhesiveness and shear resistance under hydrodynamic forceIn vitro selection of RNA aptamers against a composite small molecule-protein surfaceLatent TGF-β structure and activationThe structure of the SBP-Tag-streptavidin complex reveals a novel helical scaffold bridging binding pockets on separate subunits.Structure of Bradavidin – C-Terminal Residues Act as Intrinsic LigandsPurification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7p.Yos9p and Hrd1p mediate ER retention of misfolded proteins for ER-associated degradation.C-terminal phosphorylation of Hsp70 and Hsp90 regulates alternate binding to co-chaperones CHIP and HOP to determine cellular protein folding/degradation balancesA versatile viral system for expression and depletion of proteins in mammalian cellsA chloride conductance in VGLUT1 underlies maximal glutamate loading into synaptic vesiclesPlasmid vectors for proteomic analyses in Giardia: purification of virulence factors and analysis of the proteasomeAccelerated neuronal cell recovery from Botulinum neurotoxin intoxication by targeted ubiquitinationProduction and characterization of monoclonal antibodies sensitive to conformation in the 5HT2c serotonin receptor.Recombinant protein expression and purification: a comprehensive review of affinity tags and microbial applications.Proteomic analysis of the androgen receptor via MS-compatible purification of biotinylated protein on streptavidin resin.Drosophila Kelch functions with Cullin-3 to organize the ring canal actin cytoskeletonCamelid single domain antibodies (VHHs) as neuronal cell intrabody binding agents and inhibitors of Clostridium botulinum neurotoxin (BoNT) proteasesYersinia virulence factor YopM induces sustained RSK activation by interfering with dephosphorylation.SpyAvidin hubs enable precise and ultrastable orthogonal nanoassembly.General approach for characterizing in vitro selected peptides with protein binding affinity.Structure-guided design of an engineered streptavidin with reusability to purify streptavidin-binding peptide tagged proteins or biotinylated proteins.The RNA binding protein RBM38 (RNPC1) regulates splicing during late erythroid differentiation.Reinvestigation of aminoacyl-tRNA synthetase core complex by affinity purification-mass spectrometry reveals TARSL2 as a potential member of the complex.Biosynthesis and immobilization of biofunctional allophycocyanin.Direct comparison of linear and macrocyclic compound libraries as a source of protein ligandsAntibody-free magnetic cell sorting of genetically modified primary human CD4+ T cells by one-step streptavidin affinity purificationAndrogen receptor and chemokine receptors 4 and 7 form a signaling axis to regulate CXCL12-dependent cellular motilityA phospho-proteomic screen identifies substrates of the checkpoint kinase Chk1.A New Versatile Immobilization Tag Based on the Ultra High Affinity and Reversibility of the Calmodulin-Calmodulin Binding Peptide Interaction.Engineering Streptavidin and a Streptavidin-Binding Peptide with Infinite Binding Affinity and Reversible Binding Capability: Purification of a Tagged Recombinant Protein to High Purity via Affinity-Driven Thiol CouplingMolecular Characterization of LubX: Functional Divergence of the U-Box Fold by Legionella pneumophila.TRP and Rhodopsin Transport Depends on Dual XPORT ER Chaperones Encoded by an Operon.Nano-guided cell networks as conveyors of molecular communicationHighly Constrained Bicyclic Scaffolds for the Discovery of Protease-Stable Peptides via mRNA Display.Structural characterization of core-bradavidin in complex with biotin.
P2860
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P2860
One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-Tag
description
2001 nî lūn-bûn
@nan
2001 թուականի Դեկտեմբերին հրատարակուած գիտական յօդուած
@hyw
2001 թվականի դեկտեմբերին հրատարակված գիտական հոդված
@hy
2001年の論文
@ja
2001年論文
@yue
2001年論文
@zh-hant
2001年論文
@zh-hk
2001年論文
@zh-mo
2001年論文
@zh-tw
2001年论文
@wuu
name
One-step purification of recom ...... n-binding peptide, the SBP-Tag
@ast
One-step purification of recom ...... n-binding peptide, the SBP-Tag
@en
One-step purification of recom ...... n-binding peptide, the SBP-Tag
@nl
type
label
One-step purification of recom ...... n-binding peptide, the SBP-Tag
@ast
One-step purification of recom ...... n-binding peptide, the SBP-Tag
@en
One-step purification of recom ...... n-binding peptide, the SBP-Tag
@nl
prefLabel
One-step purification of recom ...... n-binding peptide, the SBP-Tag
@ast
One-step purification of recom ...... n-binding peptide, the SBP-Tag
@en
One-step purification of recom ...... n-binding peptide, the SBP-Tag
@nl
P2093
P3181
P356
P1476
One-step purification of recom ...... n-binding peptide, the SBP-Tag
@en
P2093
P3181
P356
10.1006/PREP.2001.1515
P407
P577
2001-12-01T00:00:00Z