Characterization and purification of a phage phi 29-encoded DNA polymerase required for the initiation of replication
about
Design parameters to control synthetic gene expression in Escherichia coliDesign and cloning strategies for constructing shRNA expression vectors.Multiple displacement amplification for complex mixtures of DNA fragmentsPhi29 DNA polymerase residues Tyr59, His61 and Phe69 of the highly conserved ExoII motif are essential for interaction with the terminal proteinA positively charged residue of phi29 DNA polymerase, highly conserved in DNA polymerases from families A and B, is involved in binding the incoming nucleotide.The phi29 DNA polymerase:protein-primer structure suggests a model for the initiation to elongation transitionDNA-Binding Proteins Essential for Protein-Primed Bacteriophage Φ29 DNA ReplicationSingle Cell Isolation and AnalysisBacteriophage protein-protein interactionsThe highly conserved amino acid sequence motif Tyr-Gly-Asp-Thr-Asp-Ser in alpha-like DNA polymerases is required by phage phi 29 DNA polymerase for protein-primed initiation and polymerizationAn essential arginine residue for initiation of protein-primed DNA replicationStructural and functional relationships between prokaryotic and eukaryotic DNA polymerases.Site-directed mutagenesis at the Exo III motif of phi 29 DNA polymerase; overlapping structural domains for the 3'-5' exonuclease and strand-displacement activities.Phi29 family of phages.The future is now: single-cell genomics of bacteria and archaeaRelating structure to function in phi29 DNA polymerase.Dynamic relocalization of phage phi 29 DNA during replication and the role of the viral protein p16.7.Replication of phage phi 29 DNA in vitro: role of the viral protein p6 in initiation and elongationDissecting the role of the ϕ29 terminal protein DNA binding residues in viral DNA replication.Characterization of the phage phi 29 protein p5 as a single-stranded DNA binding protein. Function in phi 29 DNA-protein p3 replicationRegions at the carboxyl end of bacteriophage phi 29 protein p6 required for DNA binding and activity in phi 29 DNA replication.Structural and functional analysis of temperature-sensitive mutants of the phage phi 29 DNA polymerase.Disclosing the in vivo organization of a viral histone-like protein in Bacillus subtilis mediated by its capacity to recognize the viral genomeFunctional domains in the bacteriophage phi 29 terminal protein for interaction with the phi 29 DNA polymerase and with DNATerminal protein-primed DNA amplificationSite-directed mutagenesis in the DNA linking site of bacteriophage phi 29 terminal protein: isolation and characterization of a Ser232----Thr mutantCharacterization of the origins of replication of bacteriophage phi 29 DNA.Characterization of a 3'----5' exonuclease activity in the phage phi 29-encoded DNA polymeraseOverproduction and purification of protein P6 of Bacillus subtilis phage phi 29: role in the initiation of DNA replicationTransition from initiation to elongation in protein-primed phi 29 DNA replication: salt-dependent stimulation by the viral protein p6.Effect of NH4+ ions on phi 29 DNA-protein p3 replication: formation of a complex between the terminal protein and the DNA polymeraseSomething from (almost) nothing: the impact of multiple displacement amplification on microbial ecology.Initiation of phi 29 DNA replication occurs at the second 3' nucleotide of the linear template: a sliding-back mechanism for protein-primed DNA replication.Interaction of the bacteriophage phi 29 protein p6 with double-stranded DNA.Replication of phage phi 29 DNA with purified terminal protein and DNA polymerase: synthesis of full-length phi 29 DNA.Genomic sequencing of uncultured microorganisms from single cells.Single-cell analysis of the transcriptome and its application in the characterization of stem cells and early embryos.Decoding neural transcriptomes and epigenomes via high-throughput sequencing.Primer terminus stabilization at the phi 29 DNA polymerase active site. Mutational analysis of conserved motif KXY.Microbiota of deciduous endodontic infections analysed by MDA and Checkerboard DNA-DNA hybridization.
P2860
Q21090049-46256860-F825-422A-B6D7-0146374C27F3Q21256661-1137E6D1-EEFE-420B-B98B-19ABEC579C81Q21263169-84470085-8CE5-40D5-B549-FFE1B8E3AE97Q24514925-7C33F799-BF84-47B3-A41D-BDDA7DDD1706Q24515042-34C9ED8D-1FEB-4100-B18D-46CB4B3D9FA1Q24542010-A274E198-72DF-4BC5-B486-E2D4B95475A3Q26740010-A367656F-3E02-4F34-B72B-19585F91DD62Q28078083-7690C43C-4B46-458A-A158-EFF93F351884Q28269950-D0D2716C-DB32-4DE7-8637-4BACA20F1370Q33635485-E396F058-2003-47B7-99CE-5039CB80D60CQ33884833-33AF2708-4737-48DD-8300-1C1B6C83001FQ33930162-CA6B8EEF-C51D-429A-BB86-6EE087D8C303Q33938548-838549D9-0526-403F-81C8-91118289621DQ34010356-F636D164-626D-45ED-99E5-ED33CB8DA3E3Q34321477-0B05513F-894E-4A41-BA4B-CE0B4B429E90Q34377993-FB2BAE53-7669-4AFC-B4A8-44FCFF92B5BCQ34732810-24FB8438-E6E1-4E73-88A0-831D3D5565FAQ35045301-032967CA-4571-49FD-A6DB-BB0BFF679516Q35171623-BFA69043-0829-43D1-B860-AA87A32369E3Q35227590-F013664F-BE48-4105-96D6-D2ACE15D6654Q35232445-944A857C-7A10-411F-8D22-AA49C2865019Q35870355-B8097793-7D99-407A-BE61-906E181527D5Q35889500-5D40F415-3E48-4CC1-BF97-E3B76B4E5ACEQ35962719-C8E6908C-9050-4F02-AD7E-8ACCDC153E98Q35966978-6839217C-BC82-436A-B797-939153FCAE95Q36011186-E203DEA4-C282-4903-8B8B-32FE444A18F1Q36011556-C11525CD-96BB-4E93-991B-11270BF7E80AQ36136289-FAF215C5-D3C6-4AB9-8665-40A795D716BBQ36141406-25C2B360-12F0-4194-9D83-DCA5D8101530Q36881855-835463CE-9F4D-4F6A-90DF-09C0A80467A7Q36920528-01311FA2-DC93-49B9-935A-0A4ECF4F1A33Q37078861-77B0C056-C162-4C67-B45C-93E686A477BEQ37245086-1A0463B6-CA3B-442B-9298-690A11BA9F70Q37481681-9691D457-27CD-4AD3-985D-DBBC75BCF8D9Q37533559-8686463D-2930-4E27-8013-A190F3CAF79CQ38034380-B1271181-E85D-487B-8AAD-F0672365C7DDQ38197950-3FDD1A6C-5013-4113-B56D-99741FDF18E1Q38263227-BD7DB136-9134-4ED8-90F6-2CEA6DC72D6BQ38298894-EDB309B4-78F8-4952-BBC5-1FAAD41C300BQ38536331-50A51C81-B25A-4BB0-884C-85AA7FBBF668
P2860
Characterization and purification of a phage phi 29-encoded DNA polymerase required for the initiation of replication
description
1984 nî lūn-bûn
@nan
1984 թուականի Սեպտեմբերին հրատարակուած գիտական յօդուած
@hyw
1984 թվականի սեպտեմբերին հրատարակված գիտական հոդված
@hy
1984年の論文
@ja
1984年論文
@yue
1984年論文
@zh-hant
1984年論文
@zh-hk
1984年論文
@zh-mo
1984年論文
@zh-tw
1984年论文
@wuu
name
Characterization and purificat ...... the initiation of replication
@ast
Characterization and purificat ...... the initiation of replication
@en
Characterization and purificat ...... the initiation of replication
@nl
type
label
Characterization and purificat ...... the initiation of replication
@ast
Characterization and purificat ...... the initiation of replication
@en
Characterization and purificat ...... the initiation of replication
@nl
prefLabel
Characterization and purificat ...... the initiation of replication
@ast
Characterization and purificat ...... the initiation of replication
@en
Characterization and purificat ...... the initiation of replication
@nl
P2860
P3181
P356
P1476
Characterization and purificat ...... the initiation of replication
@en
P2093
P2860
P304
P3181
P356
10.1073/PNAS.81.17.5325
P407
P577
1984-09-01T00:00:00Z