Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity - Wikidata - awgldk - TriplyDB

Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity

Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity

http://www.wikidata.org/entity/Q28295016

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Engineering therapeutic antibodies targeting G-protein-coupled receptors Large-scale production and protein engineering of G protein-coupled receptors for structural studies Fluorescent approaches for understanding interactions of ligands with G protein coupled receptors Library methods for structural biology of challenging proteins and their complexes Structure of signaling-competent neurotensin receptor 1 obtained by directed evolution in Escherichia coli Rapid Bioinformatic Identification of Thermostabilizing Mutations Translation levels control multi-spanning membrane protein expression Escherichia coli as host for membrane protein structure determination: a global analysis.G-protein-coupled receptor structures were not built in a day Naturally evolved G protein-coupled receptors adopt metastable conformations.Protein engineering methods applied to membrane protein targets.Computationally-predicted CB1 cannabinoid receptor mutants show distinct patterns of salt-bridges that correlate with their level of constitutive activity reflected in G protein coupling levels, thermal stability, and ligand binding.Selection and characterization of DARPins specific for the neurotensin receptor 1 Tuning microbial hosts for membrane protein production.Simple genetic selection protocol for isolation of overexpressed genes that enhance accumulation of membrane-integrated human G protein-coupled receptors in Escherichia coli Comprehensive engineering of Escherichia coli for enhanced expression of IgG antibodies Engineering cells to improve protein expression.Multi-copy genes that enhance the yield of mammalian G protein-coupled receptors in Escherichia coli.Critical features for biosynthesis, stability, and functionality of a G protein-coupled receptor uncovered by all-versus-all mutations.Functional fusions of T4 lysozyme in the third intracellular loop of a G protein-coupled receptor identified by a random screening approach in yeast.Transient and stable expression of the neurotensin receptor NTS1: a comparison of the baculovirus-insect cell and the T-REx-293 expression systems.Strain engineering for improved expression of recombinant proteins in bacteria High-resolution modeling of transmembrane helical protein structures from distant homologues Comprehensive analysis of heterotrimeric G-protein complex diversity and their interactions with GPCRs in solution.Rapid isolation of antibody from a synthetic human antibody library by repeated fluorescence-activated cell sorting (FACS)Stabilization of G protein-coupled receptors by point mutations.Identification of destabilizing and stabilizing mutations of Ste2p, a G protein-coupled receptor in Saccharomyces cerevisiae.Conceptual and methodological advances in cell-free directed evolution.High throughput engineering to revitalize a vestigial electron transfer pathway in bacterial photosynthetic reaction centers Structural prerequisites for G-protein activation by the neurotensin receptor Enhanced secretion of recombinant proteins via signal recognition particle (SRP)-dependent secretion pathway by deletion of rrsE in Escherichia coli.Structure-guided SCHEMA recombination generates diverse chimeric channelrhodopsins Purification of recombinant G-protein-coupled receptors.Directed evolution of G protein-coupled receptors in yeast for higher functional production in eukaryotic expression hosts.Optimising the combination of thermostabilising mutations in the neurotensin receptor for structure determination.Engineering G protein-coupled receptor expression in bacteria Conformational dynamics of a G-protein α subunit is tightly regulated by nucleotide binding.Toward high-resolution computational design of the structure and function of helical membrane proteins Adenovirus mediated expression "in vivo" of the chemokine receptor CXCR1 A Link between Integral Membrane Protein Expression and Simulated Integration Efficiency

P2860

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P2860

Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity

http://www.wikidata.org/entity/Q28295016

description

2008 nî lūn-bûn

@nan

2008 թուականի Սեպտեմբերին հրատարակուած գիտական յօդուած

@hyw

2008 թվականի սեպտեմբերին հրատարակված գիտական հոդված

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2008年の論文

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2008年論文

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2008年論文

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2008年論文

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2008年論文

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2008年論文

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2008年论文

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name

Directed evolution of a G prot ...... ility, and binding selectivity

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Directed evolution of a G prot ...... ility, and binding selectivity

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Directed evolution of a G prot ...... ility, and binding selectivity

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type

label

Directed evolution of a G prot ...... ility, and binding selectivity

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Directed evolution of a G prot ...... ility, and binding selectivity

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Directed evolution of a G prot ...... ility, and binding selectivity

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prefLabel

Directed evolution of a G prot ...... ility, and binding selectivity

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Directed evolution of a G prot ...... ility, and binding selectivity

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Directed evolution of a G prot ...... ility, and binding selectivity

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PNAS.0803103105

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Proceedings of the National Academy of Sciences of the United States of America

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Directed evolution of a G prot ...... ility, and binding selectivity

@en

P2093

Anja Mohr

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Casim A Sarkar

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Emmanuel Hermans

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Igor Dodevski

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Manca Kenig

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P2860

The Protein Data Bank

Biochemical and pharmacological profile of a potent and selective nonpeptide antagonist of the neurotensin receptor

Analysis of mutation in human cells by using an Epstein-Barr virus shuttle system

High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor

Crystal structure of rhodopsin: A G protein-coupled receptor

Crystal structure of the human beta2 adrenergic G-protein-coupled receptor

Constitutive activation of the neurotensin receptor 1 by mutation of Phe(358) in Helix seven

A "silent" polymorphism in the MDR1 gene changes substrate specificity

Isolation of high-affinity ligand-binding proteins by periplasmic expression with cytometric screening (PECS).

Engineering membrane protein overproduction in Escherichia coli.

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14808-13

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scholarly article

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4876765

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10.1073/PNAS.0803103105

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39

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105

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Andreas Plückthun

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2008-09-30T00:00:00Z

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P5875

23277544

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18812512

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P921

G protein-coupled receptor

directed evolution

protein evolution

P932

2567449

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