Expression of deuterium-isotope-labelled protein in the yeast pichia pastoris for NMR studies.
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X-ray Crystallographic and Solution State Nuclear Magnetic Resonance Spectroscopic Investigations of NADP + Binding to Ferredoxin NADP Reductase from Pseudomonas aeruginosa † , ‡Efficient production of (2)H, (13)C, (15)N-enriched industrial enzyme Rhizopus chinensis lipase with native disulfide bonds.Large crystal growth by thermal control allows combined X-ray and neutron crystallographic studies to elucidate the protonation states in Aspergillus flavus urate oxidase.Solution structure of the complex between poxvirus-encoded CC chemokine inhibitor vCCI and human MIP-1beta.Production and analysis of perdeuterated lipids from Pichia pastoris cellsMethyl labeling and TROSY NMR spectroscopy of proteins expressed in the eukaryote Pichia pastoris.Recent developments in solid-state magic-angle spinning, nuclear magnetic resonance of fully and significantly isotopically labelled peptides and proteinsExtending the size of protein-RNA complexes studied by nuclear magnetic resonance spectroscopy.Nitrogen detected TROSY at high field yields high resolution and sensitivity for protein NMRAntimalarial activity of cupredoxins: the interaction of Plasmodium merozoite surface protein 119 (MSP119) and rusticyaninCrystallization of a fungal lytic polysaccharide monooxygenase expressed from glycoengineered Pichia pastoris for X-ray and neutron diffraction.Deuterium-resistant algal cell line for D labeling of heterotrophs expresses enhanced level of Hsp60 in D2O medium.Recent Advances in the Application of Solution NMR Spectroscopy to Multi-Span Integral Membrane Proteins.Neutron scattering techniques and applications in structural biology.Perdeuteration and methyl-selective (1)H, (13)C-labeling by using a Kluyveromyces lactis expression system.A cost-effective amino-acid-type selective isotope labeling of proteins expressed in Leishmania tarentolae.Yeast-expressed human membrane protein aquaporin-1 yields excellent resolution of solid-state MAS NMR spectra.Biosynthesis and spectroscopic characterization of 2-TM fragments encompassing the sequence of a human GPCR, the Y4 receptor.High-Efficiency Expression of Yeast-Derived G-Protein Coupled Receptors and 19F Labeling for Dynamical Studies.13 C metabolic flux profiling of Pichia pastoris grown in aerobic batch cultures on glucose revealed high relative anabolic use of TCA cycle and limited incorporation of provided precursors of branched-chain amino acids.Uniform isotope labeling of a eukaryotic seven-transmembrane helical protein in yeast enables high-resolution solid-state NMR studies in the lipid environmentLate metabolic precursors for selective aromatic residue labeling
P2860
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P2860
Expression of deuterium-isotope-labelled protein in the yeast pichia pastoris for NMR studies.
description
2000 nî lūn-bûn
@nan
2000 թուականի Օգոստոսին հրատարակուած գիտական յօդուած
@hyw
2000 թվականի օգոստոսին հրատարակված գիտական հոդված
@hy
2000年の論文
@ja
2000年論文
@yue
2000年論文
@zh-hant
2000年論文
@zh-hk
2000年論文
@zh-mo
2000年論文
@zh-tw
2000年论文
@wuu
name
Expression of deuterium-isotop ...... chia pastoris for NMR studies.
@ast
Expression of deuterium-isotop ...... chia pastoris for NMR studies.
@en
type
label
Expression of deuterium-isotop ...... chia pastoris for NMR studies.
@ast
Expression of deuterium-isotop ...... chia pastoris for NMR studies.
@en
prefLabel
Expression of deuterium-isotop ...... chia pastoris for NMR studies.
@ast
Expression of deuterium-isotop ...... chia pastoris for NMR studies.
@en
P2093
P356
P1476
Expression of deuterium-isotop ...... chia pastoris for NMR studies.
@en
P2093
P2888
P304
P356
10.1023/A:1008313530207
P577
2000-08-01T00:00:00Z
P6179
1041699234