Studies of contacts between T7 RNA polymerase and its promoter reveal features in common with multisubunit RNA polymerases.
about
A combined in vitro/in vivo selection for polymerases with novel promoter specificitiesUse of site-specifically tethered chemical nucleases to study macromolecular reactionsStructure of a T7 RNA polymerase elongation complex at 2.9 A resolutionThe structure of a transcribing T7 RNA polymerase in transition from initiation to elongation.Structural confirmation of a bent and open model for the initiation complex of T7 RNA polymeraseObservation by fluorescence microscopy of transcription on single combed DNAReal-time observation of the transition from transcription initiation to elongation of the RNA polymerase.Identification of multiple rate-limiting steps during the human mitochondrial transcription cycle in vitroThe specificity loop of T7 RNA polymerase interacts first with the promoter and then with the elongating transcript, suggesting a mechanism for promoter clearanceThe transition to an elongation complex by T7 RNA polymerase is a multistep processT7 promoter release mediated by DNA scrunching.Transcription initiation in a single-subunit RNA polymerase proceeds through DNA scrunching and rotation of the N-terminal subdomains.Initial bubble collapse plays a key role in the transition to elongation in T7 RNA polymerase.Extended upstream A-T sequence increases T7 promoter strength.The +2 NTP binding drives open complex formation in T7 RNA polymerase.Multiple roles for the T7 promoter nontemplate strand during transcription initiation and polymerase release.Cross-linking of promoter DNA to T7 RNA polymerase does not prevent formation of a stable elongation complex.Photochemical analysis of structural transitions in DNA liquid crystals reveals differences in spatial structure of DNA molecules organized in liquid crystalline form.
P2860
Q24798345-4E105836-F000-464A-AA4C-F5907DF10750Q24806377-85B88797-0822-4D9C-A5FE-803E6C0595FCQ27639929-67C49DAE-2E59-48C1-84D1-9057D566DC64Q27652631-2D4466B3-723D-49D9-ACF9-ED3CE1583BE1Q28757373-E0B8FB91-29F4-4CB0-8658-BB47451819D8Q30476194-8136E76D-048B-4B83-A4A4-EE590E1198C1Q33564189-A37CB277-4DFE-477E-B834-E2DFC71B7ADCQ33855344-1F36CF85-1E34-44AE-AF0B-DB7F2BBA8A68Q35835024-251B5FF5-57F7-41F1-99D1-1E67089BEA9CQ35848926-DDD23F47-EDE5-400C-8395-E7A056361B3CQ39646247-1EE14732-D9BC-4065-BD9E-F45AA714ECF4Q40086846-82BB942E-62CC-440A-BDB6-70D692F825ABQ42634619-C2C48BB0-EF05-48BB-A42A-722526DD6420Q43647429-9F476DE5-1F7A-42CC-89F1-92E1527BEBA2Q44085043-D6029ABC-B510-4EBC-8FD8-0B59ACF21E24Q44540895-DE9E5DA4-D777-43F1-BBF2-6FA77966835DQ45013821-9D48E379-88D6-437E-ACD3-AB3A740B260AQ55316613-4235EA49-37D3-430A-96F0-FBD7CB9422F2
P2860
Studies of contacts between T7 RNA polymerase and its promoter reveal features in common with multisubunit RNA polymerases.
description
1999 nî lūn-bûn
@nan
1999 թուականի Ապրիլին հրատարակուած գիտական յօդուած
@hyw
1999 թվականի ապրիլին հրատարակված գիտական հոդված
@hy
1999年の論文
@ja
1999年論文
@yue
1999年論文
@zh-hant
1999年論文
@zh-hk
1999年論文
@zh-mo
1999年論文
@zh-tw
1999年论文
@wuu
name
Studies of contacts between T7 ...... multisubunit RNA polymerases.
@ast
Studies of contacts between T7 ...... multisubunit RNA polymerases.
@en
type
label
Studies of contacts between T7 ...... multisubunit RNA polymerases.
@ast
Studies of contacts between T7 ...... multisubunit RNA polymerases.
@en
prefLabel
Studies of contacts between T7 ...... multisubunit RNA polymerases.
@ast
Studies of contacts between T7 ...... multisubunit RNA polymerases.
@en
P2093
P356
P1433
P1476
Studies of contacts between T7 ...... multisubunit RNA polymerases.
@en
P2093
P304
P356
10.1021/BI982689E
P407
P577
1999-04-01T00:00:00Z