Rapid generation of incremental truncation libraries for protein engineering using alpha-phosphothioate nucleotides.
about
Nucleotide exchange and excision technology (NExT) DNA shuffling: a robust method for DNA fragmentation and directed evolutionStructural Redesign of Lipase B from Candida antarctica by Circular Permutation and Incremental TruncationDomain structure and protein interactions of the silent information regulator Sir3 revealed by screening a nested deletion library of protein fragments.Directed evolution: tailoring biocatalysts for industrial applicationsIdentifying residue-residue clashes in protein hybrids by using a second-order mean-field approach.Structural and functional modularity of proteins in the de novo purine biosynthetic pathway.Creating multiple-crossover DNA libraries independent of sequence identity.Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution.Enhanced crossover SCRATCHY: construction and high-throughput screening of a combinatorial library containing multiple non-homologous crossovers.Production of combinatorial libraries of fused genes by sequential transposition reactionsConstruction of DNA-shuffled and incrementally truncated libraries by a mutagenic and unidirectional reassembly method: changing from a substrate specificity of phospholipase to that of lipaseRepeat-length-independent broad-spectrum shuffling, a novel method of generating a random chimera library in vivo.On the structural and functional modularity of glycinamide ribonucleotide formyltransferases.FamClash: a method for ranking the activity of engineered enzymes.Laboratory-directed protein evolutionIPRO: an iterative computational protein library redesign and optimization procedureDirected evolution: an approach to engineer enzymes.STAR: predicting recombination sites from amino acid sequence.Non-homologous recombination of deoxyribonucleoside kinases from human and Drosophila melanogaster yields human-like enzymes with novel activities.Systematic exploration of active site mutations on human deoxycytidine kinase substrate specificityEngineering next generation proteases.Chimeric TALE recombinases with programmable DNA sequence specificityProtein engineering 20 years on.Nucleoside alpha-thiotriphosphates, polymerases and the exonuclease III analysis of oligonucleotides containing phosphorothioate linkages.Directed Evolution of Enzymes for Industrial Biocatalysis.Essential role of conserved DUF177A protein in plastid 23S rRNA accumulation and plant embryogenesis.Evolution and evolvability of proteins in the laboratory.Engineering chimaeric proteins from fold fragments: 'hopeful monsters' in protein design.Biomolecular engineering for nanobio/bionanotechnology.Site-saturation mutagenesis is more efficient than DNA shuffling for the directed evolution of beta-fucosidase from beta-galactosidase.Antibiotic resistance and restriction endonucleases in fecal enterococci of chamois (Rupicapra rupicapra Linnaeus, 1758).
P2860
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P2860
Rapid generation of incremental truncation libraries for protein engineering using alpha-phosphothioate nucleotides.
description
2001 nî lūn-bûn
@nan
2001 թուականի Փետրուարին հրատարակուած գիտական յօդուած
@hyw
2001 թվականի փետրվարին հրատարակված գիտական հոդված
@hy
2001年の論文
@ja
2001年論文
@yue
2001年論文
@zh-hant
2001年論文
@zh-hk
2001年論文
@zh-mo
2001年論文
@zh-tw
2001年论文
@wuu
name
Rapid generation of incrementa ...... ha-phosphothioate nucleotides.
@ast
Rapid generation of incrementa ...... ha-phosphothioate nucleotides.
@en
type
label
Rapid generation of incrementa ...... ha-phosphothioate nucleotides.
@ast
Rapid generation of incrementa ...... ha-phosphothioate nucleotides.
@en
prefLabel
Rapid generation of incrementa ...... ha-phosphothioate nucleotides.
@ast
Rapid generation of incrementa ...... ha-phosphothioate nucleotides.
@en
P2860
P356
P1476
Rapid generation of incrementa ...... pha-phosphothioate nucleotides
@en
P2093
P2860
P356
10.1093/NAR/29.4.E16
P407
P577
2001-02-01T00:00:00Z