Libraries of hybrid proteins from distantly related sequences.
about
Directed evolution of protein enzymes using nonhomologous random recombinationDirected evolution of single-chain Fv for cytoplasmic expression using the beta-galactosidase complementation assay results in proteins highly susceptible to protease degradation and aggregationNucleotide exchange and excision technology (NExT) DNA shuffling: a robust method for DNA fragmentation and directed evolutionTo get what we aim for--progress in diversity generation methodsSelective oxidation of carbolide C-H bonds by an engineered macrolide P450 mono-oxygenaseDomain structure and protein interactions of the silent information regulator Sir3 revealed by screening a nested deletion library of protein fragments.Directed evolution: tailoring biocatalysts for industrial applicationsGauging triple stores with actual biological data.Identifying residue-residue clashes in protein hybrids by using a second-order mean-field approach.A novel, high performance enzyme for starch liquefaction. Discovery and optimization of a low pH, thermostable alpha-amylase.Creating multiple-crossover DNA libraries independent of sequence identity.DNA family shuffling of hyperthermostable beta-glycosidases.Synthetic shuffling expands functional protein diversity by allowing amino acids to recombine independently.Library analysis of SCHEMA-guided protein recombination.Enhanced crossover SCRATCHY: construction and high-throughput screening of a combinatorial library containing multiple non-homologous crossovers.Production of combinatorial libraries of fused genes by sequential transposition reactionsAssembly of designed oligonucleotides as an efficient method for gene recombination: a new tool in directed evolution.Repeat-length-independent broad-spectrum shuffling, a novel method of generating a random chimera library in vivo.An intein-based genetic selection allows the construction of a high-quality library of binary patterned de novo protein sequences.On the structural and functional modularity of glycinamide ribonucleotide formyltransferases.FamClash: a method for ranking the activity of engineered enzymes.Laboratory-directed protein evolutionDirected evolution: an approach to engineer enzymes.Non-homologous recombination of deoxyribonucleoside kinases from human and Drosophila melanogaster yields human-like enzymes with novel activities.A focused antibody library for selecting scFvs expressed at high levels in the cytoplasm.Combinatorial recombination of gene fragments to construct a library of chimeras.Random dissection to select for protein split sites and its application in protein fragment complementationGolden gate shuffling: a one-pot DNA shuffling method based on type IIs restriction enzymes.Circular permutation of DNA cytosine-N4 methyltransferases: in vivo coexistence in the BcnI system and in vitro probing by hybrid formationNucleic acid evolution and minimization by nonhomologous random recombination.Prospecting metagenomic enzyme subfamily genes for DNA family shuffling by a novel PCR-based approachExtensive libraries of gene truncation variants generated by in vitro transposition.Transgenic organisms--time for conceptual diversification?Investigation of two distinct flavone synthases for plant-specific flavone biosynthesis in Saccharomyces cerevisiae.Algorithms for optimizing cross-overs in DNA shuffling.Protein engineering 20 years on.Improving industrial yeast strains: exploiting natural and artificial diversity.Single-step fermentative production of the cholesterol-lowering drug pravastatin via reprogramming of Penicillium chrysogenum.Assessing directed evolution methods for the generation of biosynthetic enzymes with potential in drug biosynthesis.Improvement of biocatalysts for industrial and environmental purposes by saturation mutagenesis
P2860
Q24563994-A1A207A8-1E41-4AE0-BABF-BB2471DB04F1Q24803075-EDDDC364-E085-4464-8F30-6B488E737637Q24816006-AD920BF0-0AF4-407A-BA18-0BAAD00D77C0Q26851750-F448E2B6-0B09-40CB-9903-4E0ACD077322Q27657793-77AA145B-2128-4B9B-AB99-BCF482A0ADCFQ27935281-E321E08F-4AD2-4FE7-A360-E342EC10A8F6Q28275218-00BFD199-DFAC-4DBA-8A93-A4CC182C0F5EQ28727129-932D4553-F22A-4B61-8670-6F18585FCB42Q30332747-1B95389A-FF9A-43D4-AA1D-2937450F5743Q30704966-84AFB3E8-BCF1-457B-9319-DE129288ADA1Q30721380-3331987B-9CAE-4301-94B1-672C078C8D72Q30841271-379A6BFC-5299-432A-9B06-A8C6D7522F57Q30869909-E56AD2AC-4D81-431D-B494-2FAFDBC902B8Q30968166-D9351DAE-A128-4DEF-8205-568A07B336D2Q31016777-5F629E40-8C2D-463B-A383-B985A75854B6Q31117011-390C89F1-2BC4-483F-88C0-63581DA8C838Q31123994-A5F3AD8D-DAB4-496A-BEE7-67C7B0F4C5E2Q31146218-8A1FA734-918F-4033-9410-9C762B394526Q31162655-6554CAC7-AE67-4D06-9DDF-01F8D3C2CE7BQ33193083-1A8F3D24-46DC-457B-AC0D-7D0CCA385F89Q33198593-99F7D3A8-61D6-4C66-9966-529044D44634Q33222949-FAC1C15E-51D0-43EF-9EB7-E5A668B02FAEQ33254710-8F8FCB26-DE0D-489C-B991-865A0FEEC369Q33286452-51568C40-40E3-482F-BA1F-A72A20DC92D0Q33307001-75F63CB6-B4B5-4FED-A4C8-C96F6A9DABE8Q33330050-CBC5881F-4008-4F99-9C74-54D01A0593A7Q33402502-EE329830-12FA-4C11-A423-E1543EB3F52DQ33444742-606DED67-3F65-446C-B263-540EA5F0C33BQ33600868-3CA6E4EF-2A48-40E9-A1CF-EDDA02A65927Q33641842-D0DA4C4B-A42B-429C-8A4D-5BA3578A145DQ33723807-9BA5F329-5D5C-46FC-A849-D44AA3FA63D6Q33740860-AB670373-8944-423B-A1EB-F94051D38E5CQ33964596-09D437E2-E856-47E7-95CE-41C77DC51BEEQ34232684-604DF075-9E9A-4041-B4BF-875059744941Q34247878-884D579E-0C47-4674-B5B0-C12F8C63BB52Q35014053-502756B1-E231-422A-8FE2-C9E55F556774Q35145909-984270F9-B72B-44AD-855C-901875B60D64Q35157097-136DE353-6D4B-478D-8425-54E0A2C059ECQ35161643-B9502C76-4616-4300-8BA7-F3D27C770143Q35196065-96E665D9-9E9A-4128-B5CB-51CFE1B1FFCB
P2860
Libraries of hybrid proteins from distantly related sequences.
description
2001 nî lūn-bûn
@nan
2001 թուականի Մայիսին հրատարակուած գիտական յօդուած
@hyw
2001 թվականի մայիսին հրատարակված գիտական հոդված
@hy
2001年の論文
@ja
2001年論文
@yue
2001年論文
@zh-hant
2001年論文
@zh-hk
2001年論文
@zh-mo
2001年論文
@zh-tw
2001年论文
@wuu
name
Libraries of hybrid proteins from distantly related sequences.
@ast
Libraries of hybrid proteins from distantly related sequences.
@en
type
label
Libraries of hybrid proteins from distantly related sequences.
@ast
Libraries of hybrid proteins from distantly related sequences.
@en
prefLabel
Libraries of hybrid proteins from distantly related sequences.
@ast
Libraries of hybrid proteins from distantly related sequences.
@en
P2093
P2860
P356
P1433
P1476
Libraries of hybrid proteins from distantly related sequences.
@en
P2093
P2860
P2888
P304
P356
10.1038/88129
P577
2001-05-01T00:00:00Z