Enhanced synthesis of poly(3-hydroxybutyrate) in recombinant Escherichia coli by means of error-prone PCR mutagenesis, saturation mutagenesis, and in vitro recombination of the type II polyhydroxyalkanoate synthase gene.
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Directed Evolution and Structural Analysis of NADPH-Dependent Acetoacetyl Coenzyme A (Acetoacetyl-CoA) Reductase from Ralstonia eutropha Reveals Two Mutations Responsible for Enhanced KineticsA microbial factory for lactate-based polyesters using a lactate-polymerizing enzymeCharacterization of mutants of a tyrosine ammonia-lyase from Rhodotorula glutinis.Altering the substrate specificity of polyhydroxyalkanoate synthase 1 derived from Pseudomonas putida GPo1 by localized semirandom mutagenesisLaboratory-directed protein evolutionBiosynthesis of lactate-containing polyesters by metabolically engineered bacteria.MtgA Deletion-Triggered Cell Enlargement of Escherichia coli for Enhanced Intracellular Polyester Accumulation.Novel polyhydroxyalkanoate copolymers produced in Pseudomonas putida by metagenomic polyhydroxyalkanoate synthases.Enhancing poly(3-hydroxyalkanoate) production in Escherichia coli by the removal of the regulatory gene arcAAlcoholytic cleavage of polyhydroxyalkanoate chains by class IV synthases induced by endogenous and exogenous ethanol.Engineered biosynthesis of biodegradable polymers.Cloning and heterologous expression of a novel subgroup of class IV polyhydroxyalkanoate synthase genes from the genus Bacillus.Alteration of chain length substrate specificity of Aeromonas caviae R-enantiomer-specific enoyl-coenzyme A hydratase through site-directed mutagenesis.Characterization of site-specific mutations in a short-chain-length/medium-chain-length polyhydroxyalkanoate synthase: in vivo and in vitro studies of enzymatic activity and substrate specificity.Expression of 3-ketoacyl-acyl carrier protein reductase (fabG) genes enhances production of polyhydroxyalkanoate copolymer from glucose in recombinant Escherichia coli JM109Chain transfer reaction catalyzed by various polyhydroxyalkanoate synthases with poly(ethylene glycol) as an exogenous chain transfer agent.Reduced peroxisomal citrate synthase activity increases substrate availability for polyhydroxyalkanoate biosynthesis in plant peroxisomes.Tailor-made type II Pseudomonas PHA synthases and their use for the biosynthesis of polylactic acid and its copolymer in recombinant Escherichia coli.Engineering of polyhydroxyalkanoate synthase by Ser477X/Gln481X saturation mutagenesis for efficient production of 3-hydroxybutyrate-based copolyesters.Natural and engineered polyhydroxyalkanoate (PHA) synthase: key enzyme in biopolyester production.Enhanced production of polyhydroxyalkanoates (PHAs) from beechwood xylan by recombinant Escherichia coli.Microbial production of poly(lactate-co-3-hydroxybutyrate) from hybrid Miscanthus-derived sugars.Variation in copolymer composition and molecular weight of polyhydroxyalkanoate generated by saturation mutagenesis of Aeromonas caviae PHA synthase.Engineered Corynebacterium glutamicum as an endotoxin-free platform strain for lactate-based polyester production.Polyhydroxyalkanoate (PHA) synthesis by class IV PHA synthases employing Ralstonia eutropha PHB(-)4 as host strain.Engineering of polyhydroxyalkanoate (PHA) synthase PhaC2Ps of Pseudomonas stutzeri via site-specific mutation for efficient production of PHA copolymers.
P2860
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P2860
Enhanced synthesis of poly(3-hydroxybutyrate) in recombinant Escherichia coli by means of error-prone PCR mutagenesis, saturation mutagenesis, and in vitro recombination of the type II polyhydroxyalkanoate synthase gene.
description
2003 nî lūn-bûn
@nan
2003 թուականի Յունուարին հրատարակուած գիտական յօդուած
@hyw
2003 թվականի հունվարին հրատարակված գիտական հոդված
@hy
2003年の論文
@ja
2003年論文
@yue
2003年論文
@zh-hant
2003年論文
@zh-hk
2003年論文
@zh-mo
2003年論文
@zh-tw
2003年论文
@wuu
name
Enhanced synthesis of poly(3-h ...... ydroxyalkanoate synthase gene.
@ast
Enhanced synthesis of poly(3-h ...... ydroxyalkanoate synthase gene.
@en
type
label
Enhanced synthesis of poly(3-h ...... ydroxyalkanoate synthase gene.
@ast
Enhanced synthesis of poly(3-h ...... ydroxyalkanoate synthase gene.
@en
prefLabel
Enhanced synthesis of poly(3-h ...... ydroxyalkanoate synthase gene.
@ast
Enhanced synthesis of poly(3-h ...... ydroxyalkanoate synthase gene.
@en
P2093
P356
P1476
Enhanced synthesis of poly(3-h ...... ydroxyalkanoate synthase gene.
@en
P2093
Kazuma Takase
Seiichi Taguchi
Yoshiharu Doi
P304
P356
10.1093/JB/MVG015
P577
2003-01-01T00:00:00Z