Overexpression, purification, and characterization of recombinant Ca-ATPase regulators for high-resolution solution and solid-state NMR studies.
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NMR solution structure and topological orientation of monomeric phospholamban in dodecylphosphocholine micellesStructure and topology of monomeric phospholamban in lipid membranes determined by a hybrid solution and solid-state NMR approachStructures of the Excited States of Phospholamban and Shifts in Their Populations upon PhosphorylationStructural Dynamics and Topology of Phosphorylated Phospholamban Homopentamer Reveal Its Role in the Regulation of Calcium TransportSimultaneous acquisition of 2D and 3D solid-state NMR experiments for sequential assignment of oriented membrane protein samplesA systematic assessment of mature MBP in membrane protein production: overexpression, membrane targeting and purification.Probing ground and excited states of phospholamban in model and native lipid membranes by magic angle spinning NMR spectroscopy.Tilt and azimuthal angles of a transmembrane peptide: a comparison between molecular dynamics calculations and solid-state NMR data of sarcolipin in lipid membranes.What can we learn from a small regulatory membrane protein?Sensitivity enhancement of separated local field experiments: application to membrane proteinsMapping the interaction surface of a membrane protein: unveiling the conformational switch of phospholamban in calcium pump regulation.(1)H/(15)N heteronuclear NMR spectroscopy shows four dynamic domains for phospholamban reconstituted in dodecylphosphocholine micelles.Comparison of the structure and function of phospholamban and the arginine-14 deficient mutant associated with dilated cardiomyopathy.Effects of naturally occurring arginine 14 deletion on phospholamban conformational dynamics and membrane interactionsSmall expression tags enhance bacterial expression of the first three transmembrane segments of the apelin receptor.Proton evolved local field solid-state nuclear magnetic resonance using Hadamard encoding: theory and application to membrane proteins.Paramagnetic-based NMR restraints lift residual dipolar coupling degeneracy in multidomain detergent-solubilized membrane proteins.Rheostatic Regulation of the SERCA/Phospholamban Membrane Protein Complex Using Non-Coding RNA and Single-Stranded DNA oligonucleotides.Spectroscopic validation of the pentameric structure of phospholambanActivating and deactivating roles of lipid bilayers on the Ca(2+)-ATPase/phospholamban complexStructural biology of transmembrane domains: efficient production and characterization of transmembrane peptides by NMR.The structural topology of wild-type phospholamban in oriented lipid bilayers using 15N solid-state NMR spectroscopyDual acquisition magic-angle spinning solid-state NMR-spectroscopy: simultaneous acquisition of multidimensional spectra of biomacromolecules.Tuning the structural coupling between the transmembrane and cytoplasmic domains of phospholamban to control sarcoplasmic reticulum Ca(2+)-ATPase (SERCA) function.A bifunctional spin label reports the structural topology of phospholamban in magnetically-aligned bicelles.Isotope labeling for solution and solid-state NMR spectroscopy of membrane proteinsSarco(endo)plasmic reticulum calcium ATPase (SERCA) inhibition by sarcolipin is encoded in its luminal tail.Structural and dynamic basis of phospholamban and sarcolipin inhibition of Ca(2+)-ATPase.Expression of membrane proteins from Mycobacterium tuberculosis in Escherichia coli as fusions with maltose binding protein.Fluidic and air-stable supported lipid bilayer and cell-mimicking microarrays.Expression and purification of isotopically labeled peptide inhibitors and substrates of cAMP-dependant protein kinase A for NMR analysisAllosteric regulation of SERCA by phosphorylation-mediated conformational shift of phospholambanDetermination of structural topology of a membrane protein in lipid bilayers using polarization optimized experiments (POE) for static and MAS solid state NMR spectroscopy.Reversal of Phospholamban Inhibition of the Sarco(endo)plasmic Reticulum Ca2+-ATPase (SERCA) Using Short, Protein-interacting RNAs and Oligonucleotide Analogs.(15)N Solid-state NMR spectroscopic studies on phospholamban at its phosphorylated form at ser-16 in aligned phospholipid bilayers.Lipid-mediated folding/unfolding of phospholamban as a regulatory mechanism for the sarcoplasmic reticulum Ca2+-ATPaseTime-resolved FRET reveals the structural mechanism of SERCA-PLB regulation.Sensitivity enhanced heteronuclear correlation spectroscopy in multidimensional solid-state NMR of oriented systems via chemical shift coherences.Isotopically labeled expression in E. coli, purification, and refolding of the full ectodomain of the influenza virus membrane fusion protein.Probing excited states and activation energy for the integral membrane protein phospholamban by NMR CPMG relaxation dispersion experiments.
P2860
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P2860
Overexpression, purification, and characterization of recombinant Ca-ATPase regulators for high-resolution solution and solid-state NMR studies.
description
2003 nî lūn-bûn
@nan
2003 թուականի Օգոստոսին հրատարակուած գիտական յօդուած
@hyw
2003 թվականի օգոստոսին հրատարակված գիտական հոդված
@hy
2003年の論文
@ja
2003年論文
@yue
2003年論文
@zh-hant
2003年論文
@zh-hk
2003年論文
@zh-mo
2003年論文
@zh-tw
2003年论文
@wuu
name
Overexpression, purification, ...... n and solid-state NMR studies.
@ast
Overexpression, purification, ...... n and solid-state NMR studies.
@en
type
label
Overexpression, purification, ...... n and solid-state NMR studies.
@ast
Overexpression, purification, ...... n and solid-state NMR studies.
@en
prefLabel
Overexpression, purification, ...... n and solid-state NMR studies.
@ast
Overexpression, purification, ...... n and solid-state NMR studies.
@en
P2093
P1476
Overexpression, purification, ...... on and solid-state NMR studies
@en
P2093
Bethany Buck
Gianluigi Veglia
Jamillah Zamoon
Tara L Kirby
Tara M DeSilva
P304
P356
10.1016/S1046-5928(03)00127-X
P577
2003-08-01T00:00:00Z