Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris.
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Metabolic engineering of Rhizopus oryzae for the production of platform chemicalsA simple Pichia pastoris fermentation and downstream processing strategy for making recombinant pandemic Swine Origin Influenza a virus Hemagglutinin protein.Optimized expression of a thermostable xylanase from Thermomyces lanuginosus in Pichia pastoris.Enhanced thermostability of a Rhizopus chinensis lipase by in vivo recombination in Pichia pastoris.Molecular cloning and heterologous expression of a true lipase in Pichia pastoris isolated via a metagenomic approach.Expression of an endo-β-1,4-glucanase gene from orpinomyces PC-2 in Pichia pastorisEvaluation of Pichia pastoris-expressed recombinant rhoptry protein 2 of Toxoplasma gondii for its application in diagnosis of toxoplasmosis.Role of N-linked glycosylation in the secretion and enzymatic properties of Rhizopus chinensis lipase expressed in Pichia pastorisOperational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: a review.Biotechnological methods to accelerate cheddar cheese ripening.High-level expression and characterization of a chimeric lipase from Rhizopus oryzae for biodiesel production.Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastorisHigh cell density fed-batch fermentations for lipase production: feeding strategies and oxygen transfer.Effects of co-overexpression of secretion helper factors on the secretion of a HSA fusion protein (IL2-HSA) in Pichia pastoris.Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipaseMetabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures.Droplet digital PCR-aided screening and characterization of Pichia pastoris multiple gene copy strains.Heterologous production of functional forms of Rhizopus oryzae lipase in Escherichia coli.Optimization of norovirus virus-like particle production in Pichia pastoris using a real-time near-infrared bioprocess monitor.Codon optimization of xylA gene for recombinant glucose isomerase production in Pichia pastoris and fed-batch feeding strategies to fine-tune bioreactor performance.Quantitative Metabolomics and Instationary 13C-Metabolic Flux Analysis Reveals Impact of Recombinant Protein Production on Trehalose and Energy Metabolism in Pichia pastorisDevelopment of a fed-batch process for a recombinant Pichia pastoris Δoch1 strain expressing a plant peroxidaseCloning and expression of Aspergillus tamarii FS132 lipase gene in Pichia pastoris.Functional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreactors, but not shake-flasks.Characterization and high level expression of acidic endoglucanase in Pichia pastoris.Efficient secretion of lipase r27RCL in Pichia pastoris by enhancing the disulfide bond formation pathway in the endoplasmic reticulum.A molecular mechanism of enantiorecognition of tertiary alcohols by carboxylesterases.A proteomic analysis of the Pichia pastoris secretome in methanol-induced cultures.Amino acid biosynthesis and metabolic flux profiling of Pichia pastoris.Codon modification for the DNA sequence of a single-chain Fv antibody against clenbuterol and expression in Pichia pastoris.Small-scale slow glucose feed cultivation of Pichia pastoris without repression of AOX1 promoter: towards high throughput cultivations.Effect of dilution rate and methanol-glycerol mixed feeding on heterologous Rhizopus oryzae lipase production with Pichia pastoris Mut(+) phenotype in continuous culture.Cloning and expression of a xylanase xynB from Aspergillus niger IA-001 in Pichia pastoris.Design of a novel automated methanol feed system for pilot-scale fermentation of Pichia pastoris.Optimized growth kinetics of Pichia pastoris and recombinant Candida rugosa LIP1 production by RSM.Production of a sterol esterase from Ophiostoma piceae in batch and fed-batch bioprocesses using different Pichia pastoris phenotypes as cell factory.Optimized Expression and Characterization of a Novel Fully Human Bispecific Single-Chain Diabody Targeting Vascular Endothelial Growth Factor165 and Programmed Death-1 in and Evaluation of Antitumor Activity In Vivo
P2860
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P2860
Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris.
description
2001 nî lūn-bûn
@nan
2001 թուականի Մարտին հրատարակուած գիտական յօդուած
@hyw
2001 թվականի մարտին հրատարակված գիտական հոդված
@hy
2001年の論文
@ja
2001年論文
@yue
2001年論文
@zh-hant
2001年論文
@zh-hk
2001年論文
@zh-mo
2001年論文
@zh-tw
2001年论文
@wuu
name
Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris.
@ast
Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris.
@en
type
label
Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris.
@ast
Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris.
@en
prefLabel
Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris.
@ast
Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris.
@en
P2093
P1476
Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris.
@en
P2093
P356
10.1016/S0168-1656(00)00402-8
P577
2001-03-01T00:00:00Z