Small interfering RNA production by enzymatic engineering of DNA (SPEED)
about
The glucose transporter 4-regulating protein TUG is essential for highly insulin-responsive glucose uptake in 3T3-L1 adipocytesRNA interference and potential therapeutic applications of short interfering RNAsGenome-wide screening for gene function using RNAi in mammalian cells.Silence of the genes.A random peptide library fused to CCR5 for selection of mimetopes expressed on the mammalian cell surface via retroviral vectors.Production of complex nucleic acid libraries using highly parallel in situ oligonucleotide synthesis.Gene discovery by ribozyme and siRNA libraries.Genome-wide application of RNAi to the discovery of potential drug targets.PCR-based generation of shRNA libraries from cDNAs.Enzymatically prepared RNAi libraries.Generation of RNAi libraries for high-throughput screens.High-throughput screening using siRNA (RNAi) libraries.Efficient construction of an inverted minimal H1 promoter driven siRNA expression cassette: facilitation of promoter and siRNA sequence exchange.Primer extension-based method for the generation of a siRNA/miRNA expression vector.Generation of shRNAs from randomized oligonucleotidesA random shRNA-encoding library for phenotypic selection and hit-optimization.Rolling circle amplification-mediated hairpin RNA (RMHR) library construction in plants.RNA modulators of complex phenotypes in mammalian cells.Construction of siRNA/miRNA expression vectors based on a one-step PCR processInhibitors of MyD88-dependent proinflammatory cytokine production identified utilizing a novel RNA interference screening approachFunction-based gene identification using enzymatically generated normalized shRNA library and massive parallel sequencing.Selection of potent non-toxic inhibitory sequences from a randomized HIV-1 specific lentiviral short hairpin RNA library.Targeted shRNA screening identified critical roles of pleckstrin-2 in erythropoiesis.A novel method to convert a DNA fragment inserted into a plasmid to an inverted repeat structure.Unlocking the potential of the human genome with RNA interference.Size unbiased representative enzymatically generated RNAi (SURER) library and application for RNAi therapeutic screens.Strand antagonism in RNAi: an explanation of differences in potency between intracellularly expressed siRNA and shRNARNAi and HTS: exploring cancer by systematic loss-of-function.Epigenetic manipulation of gene expression: a toolkit for cell biologistsRNA knockdown as a potential therapeutic strategy in Parkinson's disease.The RNA interference revolution.Enhancing siRNA effects in T cells for adoptive immunotherapy.Building mammalian signalling pathways with RNAi screens.RNA interference in embryonic stem cells and the prospects for future therapies.RNA-mediated gene silencing in hematopoietic cells.Vector-based siRNA delivery strategies for high-throughput screening of novel target genes.Identification of GAS1 as an epirubicin resistance-related gene in human gastric cancer cells with a partially randomized small interfering RNA libraryAntiviral RNAi: translating science towards therapeutic success.Drug target discovery using retroviruses.Introduction of mismatches in a random shRNA-encoding library improves potency for phenotypic selection.
P2860
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P2860
Small interfering RNA production by enzymatic engineering of DNA (SPEED)
description
2004 nî lūn-bûn
@nan
2004 թուականի Մարտին հրատարակուած գիտական յօդուած
@hyw
2004 թվականի մարտին հրատարակված գիտական հոդված
@hy
2004年の論文
@ja
2004年論文
@yue
2004年論文
@zh-hant
2004年論文
@zh-hk
2004年論文
@zh-mo
2004年論文
@zh-tw
2004年论文
@wuu
name
Small interfering RNA production by enzymatic engineering of DNA (SPEED)
@ast
Small interfering RNA production by enzymatic engineering of DNA (SPEED)
@en
type
label
Small interfering RNA production by enzymatic engineering of DNA (SPEED)
@ast
Small interfering RNA production by enzymatic engineering of DNA (SPEED)
@en
prefLabel
Small interfering RNA production by enzymatic engineering of DNA (SPEED)
@ast
Small interfering RNA production by enzymatic engineering of DNA (SPEED)
@en
P2860
P356
P1476
Small interfering RNA production by enzymatic engineering of DNA (SPEED)
@en
P2093
Amanda D Heard
P2860
P304
P356
10.1073/PNAS.0400551101
P407
P577
2004-03-15T00:00:00Z