High-throughput screening methodology for the directed evolution of glycosyltransferases.
about
Accurate microfluidic sorting of droplets at 30 kHz.Enzymatic methods for glyco(diversification/randomization) of drugs and small moleculesTeaching old enzymes new tricks: engineering and evolution of glycosidases and glycosyl transferases for improved glycoside synthesisBright fluorescence monitoring system utilizing Zoanthus sp. green fluorescent protein (ZsGreen) for human G-protein-coupled receptor signaling in microbial yeast cellsSingle-cell analysis and sorting using droplet-based microfluidicsVesicles tethered to microbubbles by hybridized DNA oligonucleotides: flow cytometry analysis of this new drug delivery vehicle design.Natural-product sugar biosynthesis and enzymatic glycodiversification.Flow cytometry for drug discovery, receptor pharmacology and high-throughput screeningThe great screen anomaly--a new frontier in product discovery through functional metagenomics.Probing the aglycon promiscuity of an engineered glycosyltransferaseThe impact of enzyme engineering upon natural product glycodiversificationA flow cytometry-based screen for synthetic riboswitches.One in a million: flow cytometric sorting of single cell-lysate assays in monodisperse picolitre double emulsion droplets for directed evolution.Toward a high-throughput screening platform for directed evolution of enzymes that activate genotoxic prodrugs.An efficient high-throughput screening assay for rapid directed evolution of halohydrin dehalogenase for preparation of β-substituted alcohols.Semirational Directed Evolution of Loop Regions in Aspergillus japonicus β-Fructofuranosidase for Improved Fructooligosaccharide ProductionIn vitro flow cytometry-based screening platform for cellulase engineering.Characterization of α2,3- and α2,6-sialyltransferases from Helicobacter acinonychis.Converting Pasteurella multocidaα2-3-sialyltransferase 1 (PmST1) to a regioselective α2-6-sialyltransferase by saturation mutagenesis and regioselective screening.Discovery of glycosyltransferases using carbohydrate arrays and mass spectrometry.A continuous kinetic assay for protein and DNA methyltransferase enzymatic activities.Sialic acid metabolism and sialyltransferases: natural functions and applications.Toward automated oligosaccharide synthesis.Recent biotechnological progress in enzymatic synthesis of glycosides.Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently.Solubilization and Iterative Saturation Mutagenesis of α1,3-fucosyltransferase from Helicobacter pylori to enhance its catalytic efficiency.Magnetic sensing platform technologies for biomedical applications.Glycosyltransferase engineering for carbohydrate synthesis.Development of a universal glycosyltransferase assay amenable to high-throughput formats.Stereoselective synthesis of sugar nucleotides using neighboring group participation.High Throughput Screening and Selection Methods for Directed Enzyme EvolutionStructure-based mutagenic analysis of mechanism and substrate specificity in mammalian glycosyltransferases: porcine ST3Gal-I.Lipophilic sugar nucleotide synthesis by structure-based design of nucleotidylyltransferase substrates.Facile Alkaline Lysis of Escherichia coli Cells in High-Throughput Mode for Screening Enzyme Mutants: Arylsulfatase as an Example.A sweet success for substrate engineering.Single point mutations enhance activity of cis-epoxysuccinate hydrolase.Rapid selection of sperm with high DNA integrity.Development of a high-throughput screening method for recombinant Escherichia coli with intracellular dextransucrase activity.Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform.Engineering the product profile of a polysialyltransferase.
P2860
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P2860
High-throughput screening methodology for the directed evolution of glycosyltransferases.
description
2006 nî lūn-bûn
@nan
2006 թուականի Օգոստոսին հրատարակուած գիտական յօդուած
@hyw
2006 թվականի օգոստոսին հրատարակված գիտական հոդված
@hy
2006年の論文
@ja
2006年論文
@yue
2006年論文
@zh-hant
2006年論文
@zh-hk
2006年論文
@zh-mo
2006年論文
@zh-tw
2006年论文
@wuu
name
High-throughput screening methodology for the directed evolution of glycosyltransferases.
@ast
High-throughput screening methodology for the directed evolution of glycosyltransferases.
@en
type
label
High-throughput screening methodology for the directed evolution of glycosyltransferases.
@ast
High-throughput screening methodology for the directed evolution of glycosyltransferases.
@en
prefLabel
High-throughput screening methodology for the directed evolution of glycosyltransferases.
@ast
High-throughput screening methodology for the directed evolution of glycosyltransferases.
@en
P2093
P50
P356
P1433
P1476
High-throughput screening methodology for the directed evolution of glycosyltransferases.
@en
P2093
Cecilia P C Chiu
Hongming Chen
Karena Thieme
Natalie C J Strynadka
Sabrina Buchini
Stephen G Withers
P2888
P304
P356
10.1038/NMETH899
P577
2006-08-01T00:00:00Z
P5875
P6179
1029083490