Enzyme engineering for enantioselectivity: from trial-and-error to rational design?
about
Directed evolution strategies for enantiocomplementary haloalkane dehalogenases: from chemical waste to enantiopure building blocks.Site-saturation mutagenesis: a powerful tool for structure-based design of combinatorial mutation libraries.Revisiting the lipase from Pseudomonas aeruginosa: directed evolution of substrate acceptance and enantioselectivity using iterative saturation mutagenesis.Manipulating the expression rate and enantioselectivity of an epoxide hydrolase by using directed evolution.Enhanced refoldability and thermoactivity of fluorinated phosphotriesterase.Enhancing the thermal robustness of an enzyme by directed evolution: least favorable starting points and inferior mutants can map superior evolutionary pathways.Combinatorial reshaping of the Candida antarctica lipase A substrate pocket for enantioselectivity using an extremely condensed library.Many pathways in laboratory evolution can lead to improved enzymes: how to escape from local minima.First co-expression of a lipase and its specific foldase obtained by metagenomicsDirected evolution by using iterative saturation mutagenesis based on multiresidue sites.Computational tools for rational protein engineering of aldolases.The "gate keeper" role of Trp222 determines the enantiopreference of diketoreductase toward 2-chloro-1-phenylethanone.Combinatorial library based engineering of Candida antarctica lipase A for enantioselective transacylation of sec-alcohols in organic solvent.Artificial metalloenzymes as catalysts in stereoselective Diels-Alder reactions.Protein design in systems metabolic engineering for industrial strain development.Achieving regio- and enantioselectivity of P450-catalyzed oxidative CH activation of small functionalized molecules by structure-guided directed evolution.Engineering cofactor preference of ketone reducing biocatalysts: A mutagenesis study on a γ-diketone reductase from the yeast Saccharomyces cerevisiae serving as an example.Improving biocatalyst performance by integrating statistical methods into protein engineering.Practical insights on enzyme stabilization.Thermostability improvement of a Talaromyces leycettanus xylanase by rational protein engineering.Engineering of isoamylase: improvement of protein stability and catalytic efficiency through semi-rational design.Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform.Improving the catalytic performance of a GH11 xylanase by rational protein engineering.Kinetic and thermodynamic analysis of Candida antarctica lipase B-catalyzed alcoholytic resolution of (R,S)-β-butyrolactone in organic solvents.CH-activating oxidative hydroxylation of 1-tetralones and related compounds with high regio- and stereoselectivityProtein engineering of stereoselective Baeyer-Villiger monooxygenases
P2860
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P2860
Enzyme engineering for enantioselectivity: from trial-and-error to rational design?
description
2009 nî lūn-bûn
@nan
2009 թուականի Նոյեմբերին հրատարակուած գիտական յօդուած
@hyw
2009 թվականի նոյեմբերին հրատարակված գիտական հոդված
@hy
2009年の論文
@ja
2009年論文
@yue
2009年論文
@zh-hant
2009年論文
@zh-hk
2009年論文
@zh-mo
2009年論文
@zh-tw
2009年论文
@wuu
name
Enzyme engineering for enantioselectivity: from trial-and-error to rational design?
@ast
Enzyme engineering for enantioselectivity: from trial-and-error to rational design?
@en
Enzyme engineering for enantioselectivity: from trial-and-error to rational design?
@nl
type
label
Enzyme engineering for enantioselectivity: from trial-and-error to rational design?
@ast
Enzyme engineering for enantioselectivity: from trial-and-error to rational design?
@en
Enzyme engineering for enantioselectivity: from trial-and-error to rational design?
@nl
prefLabel
Enzyme engineering for enantioselectivity: from trial-and-error to rational design?
@ast
Enzyme engineering for enantioselectivity: from trial-and-error to rational design?
@en
Enzyme engineering for enantioselectivity: from trial-and-error to rational design?
@nl
P2093
P1476
Enzyme engineering for enantioselectivity: from trial-and-error to rational design?
@en
P2093
Frank Hollmann
Isabel W C E Arends
Linda G Otten
P356
10.1016/J.TIBTECH.2009.10.001
P577
2009-11-11T00:00:00Z