Effects of polymerase, template dilution and cycle number on PCR based 16 S rRNA diversity analysis using the deep sequencing method. - Wikidata - awgldk - TriplyDB

Effects of polymerase, template dilution and cycle number on PCR based 16 S rRNA diversity analysis using the deep sequencing method.

Effects of polymerase, template dilution and cycle number on PCR based 16 S rRNA diversity analysis using the deep sequencing method.

http://www.wikidata.org/entity/Q33715118

about

The truth about metagenomics: quantifying and counteracting bias in 16S rRNA studies Towards quantitative metagenomics of wild viruses and other ultra-low concentration DNA samples: a rigorous assessment and optimization of the linker amplification method.Human gut microbiota: repertoire and variations Lessons learned and unlearned in periodontal microbiology Residual viremia in an RT-SHIV rhesus macaque HAART model marked by the presence of a predominant plasma clone and a lack of viral evolution Deep sequencing analyses of low density microbial communities: working at the boundary of accurate microbiota detection PCR-induced transitions are the major source of error in cleaned ultra-deep pyrosequencing data Synthetic spike-in standards for high-throughput 16S rRNA gene amplicon sequencing.Responses of denitrifying bacterial communities to short-term waterlogging of soils Freshwater bacterioplankton richness in oligotrophic lakes depends on nutrient availability rather than on species-area relationships.Amplification by PCR artificially reduces the proportion of the rare biosphere in microbial communities.High-throughput sequencing of 16S rRNA gene amplicons: effects of extraction procedure, primer length and annealing temperature Soil bacterial diversity screening using single 16S rRNA gene V regions coupled with multi-million read generating sequencing technologies Comparison of the levels of bacterial diversity in freshwater, intertidal wetland, and marine sediments by using millions of illumina tags.Alterations in the colonic microbiota in response to osmotic diarrhea Changes in diversity, abundance, and structure of soil bacterial communities in Brazilian Savanna under different land use systems.The effect of primer choice and short read sequences on the outcome of 16S rRNA gene based diversity studies.16S rRNA gene survey of microbial communities in Winogradsky columns Extensive recombination due to heteroduplexes generates large amounts of artificial gene fragments during PCR Effort versus Reward: Preparing Samples for Fungal Community Characterization in High-Throughput Sequencing Surveys of Soils.Manganese and iron as structuring parameters of microbial communities in Arctic marine sediments from the Baffin Bay.The vaginal microbiome is stable in prepubertal and sexually mature Ellegaard Göttingen Minipigs throughout an estrous cycle Bacterial community structure in freshwater springs infested with the invasive plant species Hydrilla verticillata Structural and functional changes within the gut microbiota and susceptibility to Clostridium difficile infection.Characterising the Canine Oral Microbiome by Direct Sequencing of Reverse-Transcribed rRNA Molecules.Metabarcoding Marine Sediments: Preparation of Amplicon Libraries.Assessment of bacterial community composition in response to uranium levels in sediment samples of sacred Cauvery River.Application of Stochastic Labeling with Random-Sequence Barcodes for Simultaneous Quantification and Sequencing of Environmental 16S rRNA Genes Genome plasticity of triple-reassortant H1N1 influenza A virus during infection of vaccinated pigs.Archaeal and bacterial communities in three alkaline hot springs in Heart Lake Geyser Basin, Yellowstone National Park.Impact of Different Fecal Processing Methods on Assessments of Bacterial Diversity in the Human Intestine.A novel ultra high-throughput 16S rRNA gene amplicon sequencing library preparation method for the Illumina HiSeq platform.Microbiome sequencing: challenges and opportunities for molecular medicine.Comparative Evaluation of Four Bacteria-Specific Primer Pairs for 16S rRNA Gene Surveys.The madness of microbiome: Attempting to find consensus "best practice" for 16S microbiome studies.Systematic Bias Introduced by Genomic DNA Template Dilution in 16S rRNA Gene-Targeted Microbiota Profiling in Human Stool Homogenates.The impact of storage buffer, DNA extraction method, and polymerase on microbial analysis.Bacterial endophytes mediate positive feedback effects of early legume termination times on the yield of subsequent durum wheat crops.Non-biological synthetic spike-in controls and the AMPtk software pipeline improve mycobiome data.The proof of concept that culturomics can be superior to metagenomics to study atypical stool samples

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Effects of polymerase, template dilution and cycle number on PCR based 16 S rRNA diversity analysis using the deep sequencing method.

http://www.wikidata.org/entity/Q33715118

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2010 nî lūn-bûn

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2010 թուականի Հոկտեմբերին հրատարակուած գիտական յօդուած

@hyw

2010 թվականի հոտեմբերին հրատարակված գիտական հոդված

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2010年の論文

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2010年論文

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2010年論文

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2010年論文

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2010年論文

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2010年論文

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2010年论文

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BMC Microbiology

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Effects of polymerase, templat ...... ng the deep sequencing method.

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Fei Zou

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Hong-Wei Zhou

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Jin-Ya Wu

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Su-Ying Lu

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Xiao-Tao Jiang

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Yun-Xia Jiang

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Pyrosequencing enumerates and contrasts soil microbial diversity

Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities

Microbial diversity in the deep sea and the underexplored "rare biosphere"

Recent developments in the optimization of thermostable DNA polymerases for efficient applications

Next-generation DNA sequencing methods

BIPES, a cost-effective high-throughput method for assessing microbial diversity.

Computational improvements reveal great bacterial diversity and high metal toxicity in soil.

Kinetic bias in estimates of coastal picoplankton community structure obtained by measurements of small-subunit rRNA gene PCR amplicon length heterogeneity

Evaluation of primers and PCR conditions for the analysis of 16S rRNA genes from a natural environment.

PCR-induced sequence artifacts and bias: insights from comparison of two 16S rRNA clone libraries constructed from the same sample.

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