A mutation in T7 RNA polymerase that facilitates promoter clearance
about
Solution structure and dynamics of the wild-type pseudoknot of human telomerase RNAThe structure of a transcribing T7 RNA polymerase in transition from initiation to elongation.Effect of pseudouridylation on the structure and activity of the catalytically essential P6.1 hairpin in human telomerase RNAStructure of a Yeast RNase III dsRBD Complex with a Noncanonical RNA Substrate Provides New Insights into Binding Specificity of dsRBDsIntrinsic Dynamics of an Extended Hydrophobic Core in the S. cerevisiae RNase III dsRBD Contributes to Recognition of Specific RNA Binding SitesStructure and sequence elements of the CR4/5 domain of medaka telomerase RNA important for telomerase functionhLARP7 C-terminal domain contains an xRRM that binds the 3' hairpin of 7SK RNAAptamer-targeted antigen delivery.Relaxed substrate specificity leads to extensive tRNA mischarging by Streptococcus pneumoniae class I and class II aminoacyl-tRNA synthetasesDifferent sequences show similar quaternary interaction stabilities in prohead viral RNA self-assemblyBridge helix and trigger loop perturbations generate superactive RNA polymerases.Functional consequences of T-stem mutations in E. coli tRNAThrUGU in vitro and in vivo.Relaxed rotational and scrunching changes in P266L mutant of T7 RNA polymerase reduce short abortive RNAs while delaying transition into elongation.Troubleshooting coupled in vitro transcription-translation system derived from Escherichia coli cells: synthesis of high-yield fully active proteins.Direct tests of the energetic basis of abortive cycling in transcription.Asymmetry of (13)C labeled 3-pyruvate affords improved site specific labeling of RNA for NMR spectroscopy.Asymmetry of 13C labeled 3-pyruvate affords improved site specific labeling of RNA for NMR spectroscopyBiomass production of site selective 13C/15N nucleotides using wild type and a transketolase E. coli mutant for labeling RNA for high resolution NMRThe transition to an elongation complex by T7 RNA polymerase is a multistep processTranscription yield of fully 2'-modified RNA can be increased by the addition of thermostabilizing mutations to T7 RNA polymerase mutants.Mechanistic characterization of the 5'-triphosphate-dependent activation of PKR: lack of 5'-end nucleobase specificity, evidence for a distinct triphosphate binding site, and a critical role for the dsRBD.New insights into the mechanism of initial transcription: the T7 RNA polymerase mutant P266L transitions to elongation at longer RNA lengths than wild typeMethods for kinetic and thermodynamic analysis of aminoacyl-tRNA synthetasesStructural conservation in the template/pseudoknot domain of vertebrate telomerase RNA from teleost fish to humanMolecular mechanism of GTPase activation at the signal recognition particle (SRP) RNA distal endStructure and folding of the Tetrahymena telomerase RNA pseudoknot.The structural changes of T7 RNA polymerase from transcription initiation to elongation.Snapshots of a viral RNA polymerase switching gears from transcription initiation to elongation.RNA synthesis and purification for structural studies.Rapid NMR screening of RNA secondary structure and binding.Integrated analysis of RNA-binding protein complexes using in vitro selection and high-throughput sequencing and sequence specificity landscapes (SEQRS).Transcription initiation in a single-subunit RNA polymerase proceeds through DNA scrunching and rotation of the N-terminal subdomains.Affinity purification of T7 RNA transcripts with homogeneous ends using ARiBo and CRISPR tagsEvaluation of 15N-detected H-N correlation experiments on increasingly large RNAs.Direct ¹³C-detected NMR experiments for mapping and characterization of hydrogen bonds in RNA.Toward an orthogonal central dogma.A T7 RNA Polymerase Mutant Enhances the Yield of 5'-Thienoguanosine-Initiated RNAs.Solution structure and dynamics of anti-CRISPR AcrIIA4, the Cas9 inhibitor.Genetic alphabet expansion transcription generating functional RNA molecules containing a five-letter alphabet including modified unnatural and natural base nucleotides by thermostable T7 RNA polymerase variants.Transient state kinetics of transcription elongation by T7 RNA polymerase.
P2860
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P2860
A mutation in T7 RNA polymerase that facilitates promoter clearance
description
2005 nî lūn-bûn
@nan
2005 թուականի Ապրիլին հրատարակուած գիտական յօդուած
@hyw
2005 թվականի ապրիլին հրատարակված գիտական հոդված
@hy
2005年の論文
@ja
2005年論文
@yue
2005年論文
@zh-hant
2005年論文
@zh-hk
2005年論文
@zh-mo
2005年論文
@zh-tw
2005年论文
@wuu
name
A mutation in T7 RNA polymerase that facilitates promoter clearance
@ast
A mutation in T7 RNA polymerase that facilitates promoter clearance
@en
type
label
A mutation in T7 RNA polymerase that facilitates promoter clearance
@ast
A mutation in T7 RNA polymerase that facilitates promoter clearance
@en
prefLabel
A mutation in T7 RNA polymerase that facilitates promoter clearance
@ast
A mutation in T7 RNA polymerase that facilitates promoter clearance
@en
P2093
P2860
P356
P1476
A mutation in T7 RNA polymerase that facilitates promoter clearance
@en
P2093
Florence Proux
Hélène Launay
Jean Guillerez
Marc Dreyfus
P2860
P304
P356
10.1073/PNAS.0407141102
P407
P577
2005-04-14T00:00:00Z