Evaluation of PCR-generated chimeras, mutations, and heteroduplexes with 16S rRNA gene-based cloning
about
Analysing diet of small herbivores: the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixturesThe murine lung microbiome in relation to the intestinal and vaginal bacterial communitiesFive simple guidelines for establishing basic authenticity and reliability of newly generated fungal ITS sequencesCloning of ribosomal ITS PCR products creates frequent, non-random chimeric sequences – a test involving heterozygotes between Gymnopus dichrous taxa I and IITwo-step total gene synthesis methodNovel high-resolution characterization of ancient DNA reveals C > U-type base modification events as the sole cause of post mortem miscoding lesionsHypothesis: Artifacts, Including Spurious Chimeric RNAs with a Short Homologous Sequence, Caused by Consecutive Reverse Transcriptions and Endogenous Random PrimersHigh-throughput metagenomic technologies for complex microbial community analysis: open and closed formatsFrom design to screening: a new antimicrobial peptide discovery pipelineITS polymorphisms shed light on hybrid evolution in apomictic plants: a case study on the Ranunculus auricomus complexIdentification of ancient remains through genomic sequencingStrategy for cloning large gene assemblages as illustrated using the phenylacetate and polyhydroxyalkanoate gene clustersThe mitochondrial genome of the legume Vigna radiata and the analysis of recombination across short mitochondrial repeats.Large genomic differences between the morphologically indistinguishable diplomonads Spironucleus barkhanus and Spironucleus salmonicida.DNA metabarcoding multiplexing and validation of data accuracy for diet assessment: application to omnivorous diet.Spatial and resource factors influencing high microbial diversity in soilComparative analysis of polychlorinated biphenyl-dechlorinating communities in enrichment cultures using three different molecular screening techniques.Heteroduplexes in mixed-template amplifications: formation, consequence and elimination by 'reconditioning PCR'Coupling of functional gene diversity and geochemical data from environmental samplesCyanobacterial diversity in natural and artificial microbial mats of Lake Fryxell (McMurdo Dry Valleys, Antarctica): a morphological and molecular approach.Extensive profiling of a complex microbial community by high-throughput sequencingHigh overall diversity and dominance of microdiverse relationships in salt marsh sulphate-reducing bacteria.Microscopic examination of distribution and phenotypic properties of phylogenetically diverse Chloroflexaceae-related bacteria in hot spring microbial mats.Recognizing diversity in coral symbiotic dinoflagellate communities.Comparison of ileum microflora of pigs fed corn-, wheat-, or barley-based diets by chaperonin-60 sequencing and quantitative PCREmpirical and theoretical bacterial diversity in four Arizona soilsDevelopment and testing of a DNA macroarray to assess nitrogenase (nifH) gene diversityMicrobial diversity and heterogeneity in sandy subsurface soils.A taxa-area relationship for bacteria.PCR-induced sequence artifacts and bias: insights from comparison of two 16S rRNA clone libraries constructed from the same sample.16S rDNA directed PCR primers and detection of methanogens in the bovine rumen.Accessing the black box of microbial diversity and ecophysiology: recent advances through polyphasic experiments.Amplification of complex gene libraries by emulsion PCR.Comparison of two fingerprinting techniques, terminal restriction fragment length polymorphism and automated ribosomal intergenic spacer analysis, for determination of bacterial diversity in aquatic environments.Community structure analyses are more sensitive to differences in soil bacterial communities than anonymous diversity indices.Microbial CO(2) fixation and sulfur cycling associated with low-temperature emissions at the Lilliput hydrothermal field, southern Mid-Atlantic Ridge (9 degrees S).Diversity of sulfate-reducing genes (dsrAB) in sediments from Puget Sound.Effect of PCR amplicon size on assessments of clone library microbial diversity and community structureSoil microbial community responses to multiple experimental climate change driversMonitoring the impact of hydrocarbon contamination and nutrient addition on microbial density, activity, and diversity in soil.
P2860
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P2860
Evaluation of PCR-generated chimeras, mutations, and heteroduplexes with 16S rRNA gene-based cloning
description
2001 nî lūn-bûn
@nan
2001 թուականի Փետրուարին հրատարակուած գիտական յօդուած
@hyw
2001 թվականի փետրվարին հրատարակված գիտական հոդված
@hy
2001年の論文
@ja
2001年学术文章
@wuu
2001年学术文章
@zh-cn
2001年学术文章
@zh-hans
2001年学术文章
@zh-my
2001年学术文章
@zh-sg
2001年學術文章
@yue
name
Evaluation of PCR-generated ch ...... th 16S rRNA gene-based cloning
@ast
Evaluation of PCR-generated ch ...... th 16S rRNA gene-based cloning
@en
type
label
Evaluation of PCR-generated ch ...... th 16S rRNA gene-based cloning
@ast
Evaluation of PCR-generated ch ...... th 16S rRNA gene-based cloning
@en
prefLabel
Evaluation of PCR-generated ch ...... th 16S rRNA gene-based cloning
@ast
Evaluation of PCR-generated ch ...... th 16S rRNA gene-based cloning
@en
P2093
P2860
P1476
Evaluation of PCR-generated ch ...... th 16S rRNA gene-based cloning
@en
P2093
A V Palumbo
J M Tiedje
P E McDonel
P2860
P304
P356
10.1128/AEM.67.2.880-887.2001
P407
P577
2001-02-01T00:00:00Z