High and low annealing temperatures increase both specificity and yield in touchdown and stepdown PCR.
about
A polymorphic gene nested within an intron of the tau gene: implications for Alzheimer's disease.Translation of a retained intron in tyrosinase-related protein (TRP) 2 mRNA generates a new cytotoxic T lymphocyte (CTL)-defined and shared human melanoma antigen not expressed in normal cells of the melanocytic lineageImmobilized probe and glass surface chemistry as variables in microarray fabricationTargeted RNA-sequencing with competitive multiplex-PCR amplicon librariesEndothelin-1 is synthesized and inhibits cyclic adenosine monophosphate- dependent anion secretion by an autocrine/paracrine mechanism in gallbladder epithelial cellsPavilion lake microbialites: morphological, molecular and biochemical evidence for a cold-water transition to colonial aggregatesUse of a three-color cDNA microarray platform to measure and control support-bound probe for improved data quality and reproducibilityHigh-performance multiplex SNP analysis of three hemochromatosis-related mutations with capillary array electrophoresis microplates.Hemi-nested touchdown PCR combined with primer-template mismatch PCR for rapid isolation and sequencing of low molecular weight glutenin subunit gene family from a hexaploid wheat BAC librarySelection strategy and the design of hybrid oligonucleotide primers for RACE-PCR: cloning a family of toxin-like sequences from Agelena orientalis.Isoform-specific effects of human apolipoprotein E on brain function revealed in ApoE knockout mice: increased susceptibility of females.Diagnostic use of PCR for detection of Pneumocystis carinii in oral wash samples.c-type cytochromes and manganese oxidation in Pseudomonas putida MnB1.Ancestry and progeny of nutrient amino acid transporters.Human immunodeficiency virus type 1 DNA sequences genetically damaged by hypermutation are often abundant in patient peripheral blood mononuclear cells and may be generated during near-simultaneous infection and activation of CD4(+) T cells.Unexpected cross-reaction with Fusobacterium necrophorum in a PCR for detection of mycoplasmas.Sensitive detection of HBsAg mutants by a gap ligase chain reaction assay.Development of a rapid real-time PCR assay for quantitation of Pneumocystis carinii f. sp. carinii.Diversity of radA genes from cultured and uncultured archaea: comparative analysis of putative RadA proteins and their use as a phylogenetic markerBioactive TGF-beta can associate with lipoproteins and is enriched in those containing apolipoprotein E3.Transformation of personal computers and mobile phones into genetic diagnostic systemsRevised selection criteria for candidate restriction enzymes in genome walkingMutant analysis shows that alanine racemases from Pseudomonas aeruginosa and Escherichia coli are dimeric.Age-associated activation of epigenetically repressed genes in the mousePCR on yeast colonies: an improved method for glyco-engineered Saccharomyces cerevisiae.Development and evaluation of a quantitative, touch-down, real-time PCR assay for diagnosing Pneumocystis carinii pneumonia.A new rapid amplification of cDNA ends method for extremely guanine plus cytosine-rich genes.Characterization of endoglin and identification of novel mutations in hereditary hemorrhagic telangiectasia.Molecular and functional analysis of Drosophila single-minded larval central brain expression.Influence of age, reproductive cycling status, and menstruation on the vaginal microbiome in baboons (Papio anubis)Expression of a novel small antimicrobial protein from the seeds of motherwort (Leonurus japonicus) confers disease resistance in tobacco.TqPCR: A Touchdown qPCR Assay with Significantly Improved Detection Sensitivity and Amplification Efficiency of SYBR Green qPCR.Identification of the MATa mating-type locus of Cryptococcus neoformans reveals a serotype A MATa strain thought to have been extinctImpact of dual infections on chemotherapeutic efficacy in BALB/c mice infected with major genotypes of Trypanosoma cruziCloning of sarco-endoplasmic reticulum Ca2+ -ATPase (SERCA) from Caribbean spiny lobster Panulirus argus.An Improved PCR-RFLP Assay for Detection and Genotyping of Asymptomatic Giardia lamblia Infection in a Resource-Poor SettingDetection of a molecular biomarker for zygomycetes by quantitative PCR assays of plasma, bronchoalveolar lavage, and lung tissue in a rabbit model of experimental pulmonary zygomycosisMolecular characterization of Aegyptianella pullorum (Rickettsiales, Anaplasmataceae)Small and dense LDL in familial combined hyperlipidemia and N291S polymorphism of the lipoprotein lipase gene.Unraveling flp-11/flp-32 dichotomy in nematodes.
P2860
Q24530700-D300CFB5-9287-47E7-BE13-F0C5EA001306Q24646800-5BF1D282-6603-44F4-9F18-FECE35BD5730Q24799977-2AD91066-882E-416B-8F83-ADB1E96E21FBQ27302929-9A15DDB1-D6A3-482F-A404-6691BBBF388FQ28379583-116E33D8-81F0-4E35-BB3B-2EA148CD17DBQ28654990-F44DE245-92E2-473C-A1C3-96D59A3987CAQ30797961-95663B3E-7AED-4041-AF93-EB14303D113AQ30982529-700178AF-6A4C-466F-8FA2-80A0EADD4A2CQ33283744-29183195-2C88-45FE-8E03-6120C877F8A6Q33284456-8B5B5C55-56F6-4B4B-9F44-B87087FF85E8Q33563135-DAD28538-5E17-4458-9E08-F50EC7C4D803Q33673833-674BF4BC-B9B3-43F2-836B-A0E2530F2161Q33715593-AA8C23EB-B1C6-4D09-8534-B6E21FC14648Q33817602-CD48DC4A-DA60-4222-BA9F-3AD90951138AQ33853620-8D347C8A-2D3E-464D-BCA6-D0D1BB10C462Q33959454-32A0B916-2C2C-420E-AC7A-A3DAC76B5AD2Q33962523-F129E60B-A381-4AFD-96FF-9666FE3B085BQ33964158-2B3AE9A1-3DD0-4F8F-B681-67BEE3178DCCQ33991157-1ADD8EB1-06AE-46A5-BBC3-8250D2668A9EQ34043609-29FFD4F4-40CD-4E3E-9B24-1E918750D4FBQ34192853-B01504EC-D439-48AF-AF13-CE2535F4C17BQ34235581-20305453-B97E-4E88-913A-680CBABADC16Q34315547-88B351C9-E667-498B-87F8-6418F3526264Q34619240-4AA70C45-F3A8-4502-BD8A-3C53F029CB7CQ34728849-AA79E8CB-FA2C-4314-AD9F-ADDB1C319E3EQ34943397-26E0D4FF-53C6-40BA-92AA-A782FE71D37DQ35104205-BB700B4D-9593-441A-A2BA-283E7BD7D489Q35248963-7E4F3A6E-41AA-4784-B61F-DB55033A76BFQ35434876-F0306900-E9A3-4501-905A-60ABBD025E52Q35560135-C9464451-EC8D-4620-9EEC-F01164C3D64EQ35642020-2312271A-B42A-4E8E-BC8D-C624C31B0827Q35690774-CF7C2592-12BD-4448-B01F-B09F0395D1DAQ35845757-E932E98B-0A01-4AC2-BD0D-5C895743E3A5Q36094866-8ABECA7A-0696-47D6-8812-E3E097A50F21Q36096832-8551AA45-1018-4AFB-AD29-F57531C1631BQ36688435-31497A26-3736-4D17-89C5-83CF6A504156Q36957985-D81EBAC4-3CE6-46FB-BA37-A50BE6C8686AQ37058285-9B2E2B24-E7C3-4805-BEE3-BE8BFB05382BQ37154843-8EA24967-A0C3-498B-9A8F-49D70C417D0AQ37289368-446E6A40-0E69-4A97-848C-E11081C4E86D
P2860
High and low annealing temperatures increase both specificity and yield in touchdown and stepdown PCR.
description
1996 nî lūn-bûn
@nan
1996 թուականի Մարտին հրատարակուած գիտական յօդուած
@hyw
1996 թվականի մարտին հրատարակված գիտական հոդված
@hy
1996年の論文
@ja
1996年論文
@yue
1996年論文
@zh-hant
1996年論文
@zh-hk
1996年論文
@zh-mo
1996年論文
@zh-tw
1996年论文
@wuu
name
High and low annealing tempera ...... in touchdown and stepdown PCR.
@ast
High and low annealing tempera ...... in touchdown and stepdown PCR.
@en
High and low annealing tempera ...... in touchdown and stepdown PCR.
@nl
type
label
High and low annealing tempera ...... in touchdown and stepdown PCR.
@ast
High and low annealing tempera ...... in touchdown and stepdown PCR.
@en
High and low annealing tempera ...... in touchdown and stepdown PCR.
@nl
prefLabel
High and low annealing tempera ...... in touchdown and stepdown PCR.
@ast
High and low annealing tempera ...... in touchdown and stepdown PCR.
@en
High and low annealing tempera ...... in touchdown and stepdown PCR.
@nl
P1433
P1476
High and low annealing tempera ...... in touchdown and stepdown PCR.
@en
P2093
P304
P577
1996-03-01T00:00:00Z