Differential priming of RNA templates during cDNA synthesis markedly affects both accuracy and reproducibility of quantitative competitive reverse-transcriptase PCR.
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PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency.With Reference to Reference Genes: A Systematic Review of Endogenous Controls in Gene Expression StudiesHigh-Throughput Single-Cell Analysis for Wound Healing Applications.Tuning pacemaker frequency of individual dopaminergic neurons by Kv4.3L and KChip3.1 transcriptionEpitope mapping using mRNA display and a unidirectional nested deletion libraryGene expression analysis of interferon kappa in laser capture microdissected cervical epitheliumSingle-cell qPCR on dispersed primary pituitary cells -an optimized protocol.Sequential analysis of global gene expression profiles in immature and in vitro matured bovine oocytes: potential molecular markers of oocyte maturationA microfluidic processor for gene expression profiling of single human embryonic stem cells.Efficient recovery of whole blood RNA--a comparison of commercial RNA extraction protocols for high-throughput applications in wildlife speciesImproved quantitative real-time RT-PCR for expression profiling of individual cells.High temporal and spatial diversity in marine RNA viruses implies that they have an important role in mortality and structuring plankton communitiesReproducibility of alternative probe synthesis approaches for gene expression profiling with arraysPitfalls of quantitative real-time reverse-transcription polymerase chain reactionQuality control methods for optimal BCR-ABL1 clinical testing in human whole blood samplesPerformance of a Taqman Assay for Improved Detection and Quantification of Human Rhinovirus Viral LoadSystematic Selection of Reference Genes for the Normalization of Circulating RNA Transcripts in Pregnant Women Based on RNA-Seq Data.The reproducibility of biomedical research: Sleepers awake!Establishment of a real-time PCR-based approach for accurate quantification of bacterial RNA targets in water, using Salmonella as a model organism.The Sporothrix schenckii Gene Encoding for the Ribosomal Protein L6 Has Constitutive and Stable Expression and Works as an Endogenous Control in Gene Expression Analysis.Characterization of in vitro transcription amplification linearity and variability in the low copy number regime using External RNA Control Consortium (ERCC) spike-ins.Quantitative evaluation of reference genes for real-time PCR during in vitro maturation of ovine oocytes.The basidiomycete Ustilago maydis has two plasma membrane H⁺-ATPases related to fungi and plants.Standard methods for molecular research inApis mellifera
P2860
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P2860
Differential priming of RNA templates during cDNA synthesis markedly affects both accuracy and reproducibility of quantitative competitive reverse-transcriptase PCR.
description
1999 nî lūn-bûn
@nan
1999 թուականի Յունուարին հրատարակուած գիտական յօդուած
@hyw
1999 թվականի հունվարին հրատարակված գիտական հոդված
@hy
1999年の論文
@ja
1999年論文
@yue
1999年論文
@zh-hant
1999年論文
@zh-hk
1999年論文
@zh-mo
1999年論文
@zh-tw
1999年论文
@wuu
name
Differential priming of RNA te ...... ive reverse-transcriptase PCR.
@ast
Differential priming of RNA te ...... ive reverse-transcriptase PCR.
@en
Differential priming of RNA te ...... ive reverse-transcriptase PCR.
@nl
type
label
Differential priming of RNA te ...... ive reverse-transcriptase PCR.
@ast
Differential priming of RNA te ...... ive reverse-transcriptase PCR.
@en
Differential priming of RNA te ...... ive reverse-transcriptase PCR.
@nl
prefLabel
Differential priming of RNA te ...... ive reverse-transcriptase PCR.
@ast
Differential priming of RNA te ...... ive reverse-transcriptase PCR.
@en
Differential priming of RNA te ...... ive reverse-transcriptase PCR.
@nl
P2860
P1433
P1476
Differential priming of RNA te ...... ive reverse-transcriptase PCR.
@en
P2093
P2860
P304
P356
10.1042/0264-6021:3370231
P407
P478
337 ( Pt 2)
P577
1999-01-01T00:00:00Z