Dissecting enzyme function with microfluidic-based deep mutational scanning
about
Causes of evolutionary rate variation among protein sitesHigh-Throughput Screening in Protein Engineering: Recent Advances and Future PerspectivesRational Protein Engineering Guided by Deep Mutational ScanningHigh-throughput screening of filamentous fungi using nanoliter-range droplet-based microfluidicsThe Generation and Exploitation of Protein Mutability Landscapes for Enzyme EngineeringFunctional Sites Induce Long-Range Evolutionary Constraints in EnzymesKinetic Characterization of 100 Glycoside Hydrolase Mutants Enables the Discovery of Structural Features Correlated with Kinetic ConstantsThermal stability and kinetic constants for 129 variants of a family 1 glycoside hydrolase reveal that enzyme activity and stability can be separately designedDetection and sequence/structure mapping of biophysical constraints to protein variation in saturated mutational libraries and protein sequence alignments with a dedicated serverA Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing.Droplet-based microfluidic high-throughput screening of heterologous enzymes secreted by the yeast Yarrowia lipolyticaMolecular Determinants of Mutant Phenotypes, Inferred from Saturation Mutagenesis DataEfficient extraction of oil from droplet microfluidic emulsionsCoevolutionary Landscape Inference and the Context-Dependence of Mutations in Beta-Lactamase TEM-1.Engineering a novel glucose-tolerant β-glucosidase as supplementation to enhance the hydrolysis of sugarcane bagasse at high glucose concentrationHigh-throughput analysis and protein engineering using microcapillary arrays.In vivo continuous evolution of genes and pathways in yeastA general strategy for expanding polymerase function by droplet microfluidics.Discovery in DropletsEmerging technologies for protease engineering: New tools to clear out disease.Mutation effects predicted from sequence co-variation.Deep sequencing methods for protein engineering and design.Rapid, chemical-free breaking of microfluidic emulsions with a hand-held antistatic gun.Are in vivo selections on the path to extinction?Droplet microfluidics for synthetic biology.Bulk double emulsification for flow cytometric analysis of microfluidic droplets.ProtaBank: A repository for protein design and engineering data.
P2860
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P2860
Dissecting enzyme function with microfluidic-based deep mutational scanning
description
2015 nî lūn-bûn
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2015年の論文
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2015年論文
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2015年論文
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2015年論文
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2015年論文
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2015年論文
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name
Dissecting enzyme function with microfluidic-based deep mutational scanning
@ast
Dissecting enzyme function with microfluidic-based deep mutational scanning
@en
type
label
Dissecting enzyme function with microfluidic-based deep mutational scanning
@ast
Dissecting enzyme function with microfluidic-based deep mutational scanning
@en
prefLabel
Dissecting enzyme function with microfluidic-based deep mutational scanning
@ast
Dissecting enzyme function with microfluidic-based deep mutational scanning
@en
P2093
P2860
P356
P1476
Dissecting enzyme function with microfluidic-based deep mutational scanning
@en
P2093
Adam R Abate
Philip A Romero
Tuan M Tran
P2860
P304
P356
10.1073/PNAS.1422285112
P407
P577
2015-05-26T00:00:00Z