A recombineering based approach for high-throughput conditional knockout targeting vector construction. - Wikidata - awgldk - TriplyDB

A recombineering based approach for high-throughput conditional knockout targeting vector construction.

A recombineering based approach for high-throughput conditional knockout targeting vector construction.

http://www.wikidata.org/entity/Q35829339

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A universal vector for high-efficiency multi-fragment recombineering of BACs and knock-in constructs Recombineering: a homologous recombination-based method of genetic engineering Sidekick 2 directs formation of a retinal circuit that detects differential motion Subtype-specific regeneration of retinal ganglion cells following axotomy: effects of osteopontin and mTOR signaling Deficiencies in the region syntenic to human 21q22.3 cause cognitive deficits in mice.Improved and simplified recombineering approach for influenza virus reverse genetics.An improved Red/ET recombineering system and mouse ES cells culture conditions for the generation of targeted mutant mice.GGNBP2 is necessary for testis morphology and sperm development A resource of vectors and ES cells for targeted deletion of microRNAs in mice.An efficient procedure for marker-free mutagenesis of S. coelicolor by site-specific recombination for secondary metabolite overproduction.Generation and characterization of a novel mouse embryonic stem cell line with a dynamic reporter of Nanog expression.Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility Senataxin plays an essential role with DNA damage response proteins in meiotic recombination and gene silencing The Igκ gene enhancers, E3' and Ed, are essential for triggering transcription VCre/VloxP and SCre/SloxP: new site-specific recombination systems for genome engineering.Characterization of the yehUT two-component regulatory system of Salmonella enterica Serovar Typhi and Typhimurium.BCL11A is a triple-negative breast cancer gene with critical functions in stem and progenitor cells.The Kil peptide of bacteriophage λ blocks Escherichia coli cytokinesis via ZipA-dependent inhibition of FtsZ assembly Subcloning plus insertion (SPI)--a novel recombineering method for the rapid construction of gene targeting vectors A modified recombineering protocol for the genetic manipulation of gene clusters in Aspergillus fumigatus.Differential requirements of singleplex and multiplex recombineering of large DNA constructs.Loss of MT1-MMP causes cell senescence and nuclear defects which can be reversed by retinoic acid Ube3a-ATS is an atypical RNA polymerase II transcript that represses the paternal expression of Ube3a Genome-wide transposon mutagenesis of Proteus mirabilis: Essential genes, fitness factors for catheter-associated urinary tract infection, and the impact of polymicrobial infection on fitness requirements.The capsular polysaccharide Vi from Salmonella typhi is a B1b antigen.Stella-Cre mice are highly efficient Cre deleters.A low-copy-number plasmid for retrieval of toxic genes from BACs and generation of conditional targeting constructs Cichlid fishes as a model to understand normal and clinical craniofacial variation Genome and transcriptome adaptation accompanying emergence of the definitive type 2 host-restricted Salmonella enterica serovar Typhimurium pathovar Endoplasmic reticulum stress-unfolding protein response-apoptosis cascade causes chondrodysplasia in a col2a1 p.Gly1170Ser mutated mouse model Genome-scale genetic manipulation methods for exploring bacterial molecular biology.Large-scale mouse knockouts and phenotypes.Introduction of temperature-sensitive helper and donor plasmids into Bac-to-Bac baculovirus expression systems.The Autographa californica Multiple Nucleopolyhedrovirus ac83 Gene Contains a cis-Acting Element That Is Essential for Nucleocapsid Assembly.Bi-allelic gene targeting in mouse embryonic stem cells Mobilization of giant piggyBac transposons in the mouse genome.Regulated selection of germinal-center cells into the memory B cell compartment.Lambda gpP-DnaB Helicase Sequestration and gpP-RpoB Associated Effects: On Screens for Auxotrophs, Selection for Rif(R), Toxicity, Mutagenicity, Plasmid Curing.ZASC1 knockout mice exhibit an early bone marrow-specific defect in murine leukemia virus replication.Efficient conditional knockout targeting vector construction using co-selection BAC recombineering (CoSBR).

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P2860

A recombineering based approach for high-throughput conditional knockout targeting vector construction.

http://www.wikidata.org/entity/Q35829339

description

2007 nî lūn-bûn

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A recombineering based approac ...... targeting vector construction.

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David Melvin

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Donald L Court

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Nina Costantino

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Pentao Liu

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Qin Su

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Ruixue Li

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Song Choon Lee

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Waiin Chan

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P2860

Initial sequencing and analysis of the human genome

The Sequence of the Human Genome

The knockout mouse project

Initial sequencing and comparative analysis of the mouse genome

The European dimension for the mouse genome mutagenesis program

Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1

Lambda Red-mediated recombinogenic engineering of enterohemorrhagic and enteropathogenic E. coli

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products

High-throughput engineering of the mouse genome coupled with high-resolution expression analysis

Use of mutated FLP recognition target (FRT) sites for the exchange of expression cassettes at defined chromosomal loci

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10.1093/NAR/GKM163

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8

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35

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17426124

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1885671

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