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Protein production and purificationTo fuse or not to fuse: what is your purpose?Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: the novel Fh8 systemMolecular cloning, overproduction, purification and biochemical characterization of the p39 nsp2 protease domains encoded by three alphavirusesNovel affinity tag system using structurally defined antibody-tag interaction: Application to single-step protein purificationThe Crystal Structure of the Orphan Nuclear Receptor NR2E3/PNR Ligand Binding Domain Reveals a Dimeric Auto-Repressed ConformationHeterologous expression of mycobacterial Esx complexes in Escherichia coli for structural studies is facilitated by the use of maltose binding protein fusionsAn intact eight-membered water chain in drosophilid alcohol dehydrogenases is essential for optimal enzyme activityEnzyme Engineering for In Situ ImmobilizationThe pCri System: a vector collection for recombinant protein expression and purificationTaking down the FLAG! How insect cell expression challenges an established tag-systemHistidine affinity tags affect MSP1(42) structural stability and immunodominance in mice.Recombinant protein expression and purification: a comprehensive review of affinity tags and microbial applications.Conformational properties of nine purified cystathionine β-synthase mutants.A peptide tag system for facile purification and single-molecule immobilizationCovering complete proteomes with X-ray structures: a current snapshot.Enhanced expression and purification of fungal galactose oxidase in Escherichia coli and use for analysis of a saturation mutagenesis library.The neurobiologist's guide to structural biology: a primer on why macromolecular structure matters and how to evaluate structural data.A phosphorylation tag for uranyl mediated protein purification and photo assisted tag removalG196 epitope tag system: a novel monoclonal antibody, G196, recognizes the small, soluble peptide DLVPR with high affinityCleavage of recombinant proteins at poly-His sequences by Co(II) and Cu(II).E. coli expression and purification of Fab antibody fragments.Fusion partners can increase the expression of recombinant interleukins via transient transfection in 2936E cellsOrthogonal protein purification facilitated by a small bispecific affinity tag.Differential temperature dependence of tobacco etch virus and rhinovirus 3C proteasesThe ability to enhance the solubility of its fusion partners is an intrinsic property of maltose-binding protein but their folding is either spontaneous or chaperone-mediatedProduction of recombinant disulfide-rich venom peptides for structural and functional analysis via expression in the periplasm of E. coli.Purification of proteins containing zinc finger domains using immobilized metal ion affinity chromatography.A tailor-made "tag-receptor" affinity pair for the purification of fusion proteins.Expanding the molecular toolbox for Lactococcus lactis: construction of an inducible thioredoxin gene fusion expression system.An overview of enzymatic reagents for the removal of affinity tags.Overview of the purification of recombinant proteins.The Xenopus laevis Atg4B Protease: Insights into Substrate Recognition and Application for Tag Removal from Proteins Expressed in Pro- and Eukaryotic Hosts.Microtubules in plantsIdentifying components of protein complexes in C. elegans using co-immunoprecipitation and mass spectrometry.Isolation of Metarhizium anisopliae carboxypeptidase A with native disulfide bonds from the cytosol of Escherichia coli BL21(DE3).Fusion-protein-assisted protein crystallizationFolding and activity of mutant cystathionine β-synthase depends on the position and nature of the purification tag: characterization of the R266K CBS mutantA Streamlined, Automated Protocol for the Production of Milligram Quantities of Untagged Recombinant Rat Lactate Dehydrogenase A Using ÄKTAxpressTM.Biological activities of histidine-rich peptides; merging biotechnology and nanomedicine.
P2860
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P2860
description
2005 nî lūn-bûn
@nan
2005年の論文
@ja
2005年学术文章
@wuu
2005年学术文章
@zh-cn
2005年学术文章
@zh-hans
2005年学术文章
@zh-my
2005年学术文章
@zh-sg
2005年學術文章
@yue
2005年學術文章
@zh
2005年學術文章
@zh-hant
name
Making the most of affinity tags.
@ast
Making the most of affinity tags.
@en
type
label
Making the most of affinity tags.
@ast
Making the most of affinity tags.
@en
prefLabel
Making the most of affinity tags.
@ast
Making the most of affinity tags.
@en
P1476
Making the most of affinity tags.
@en
P2093
David S Waugh
P304
P356
10.1016/J.TIBTECH.2005.03.012
P577
2005-06-01T00:00:00Z