Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function.
about
Identification of the site of covalent attachment of nafcillin, a reversible suicide inhibitor of beta-lactamaseCoding sequences for vasoactive intestinal peptide and PHM-27 peptide are located on two adjacent exons in the human genomeRole of flagella in adherence, internalization, and translocation of Campylobacter jejuni in nonpolarized and polarized epithelial cell culturesSite-directed mutagenesis of the proposed catalytic amino acids of the Sindbis virus capsid protein autoproteaseEngineering and Evolution of Molecular Chaperones and Protein Disaggregases with Enhanced ActivityRational Protein Engineering Guided by Deep Mutational ScanningMolecular cloning of the human cholecystokinin gene by use of a synthetic probe containing deoxyinosineA combinatorial library for the binuclear metal center of bacterial phosphotriesterase.Alterations in PBP 1A essential-for high-level penicillin resistance in Streptococcus pneumoniae.Regulation of three beta-tubulin mRNAs during rat brain development.Thiol-beta-lactamase: replacement of the active-site serine of RTEM beta-lactamase by a cysteine residue.cDNAs for the postsynaptic 43-kDa protein of Torpedo electric organ encode two proteins with different carboxyl termini.Phylogenetic group-specific oligodeoxynucleotide probes for identification of single microbial cells.Protein engineering 20 years on.Point mutations in the middle of 16S ribosomal RNA of E. coli produced by deletion loop mutagenesis.Colony probing as an alternative to standard sequencing as a means of direct analysis of chromosomal DNA to determine the spectrum of single-base changes in regions of known sequence.Site-saturation studies of beta-lactamase: production and characterization of mutant beta-lactamases with all possible amino acid substitutions at residue 71Toward computer-aided site-directed mutagenesis of enzymesUse of oligonucleotide probes to study the relatedness of delta-endotoxin genes among Bacillus thuringiensis subspecies and strainsCreation of a test plasmid for detecting G-C-to-T-A transversions by changing serine to arginine in the active site of beta-lactamase.Specific amino acid substitutions in bacterioopsin: Replacement of a restriction fragment in the structural gene by synthetic DNA fragments containing altered codonsEscherichia coli K-12 mutation that inactivates biodegradative threonine dehydratase by transposon Tn5 insertionInvolvement of both major histocompatibility complex class II alpha and beta chains in CD4 function indicates a role for ordered oligomerization in T cell activationIsolation and sequence analysis of the gene (cpdB) encoding periplasmic 2',3'-cyclic phosphodiesterase.Epidemiological study of Salmonella enteritidis strains of animal origin in Belgium.Biochemical and genetic evidence for a pseudoknot structure at the 3' terminus of the poliovirus RNA genome and its role in viral RNA amplification.Role of 3'-end sequences in infectivity of poliovirus transcripts made in vitro.Lymphotoxin activation by human T-cell leukemia virus type I-infected cell lines: role for NF-kappa B.Three poliovirus 2B mutants exhibit noncomplementable defects in viral RNA amplification and display dosage-dependent dominance over wild-type poliovirus.Activation of Ha-ras p21 by substitution, deletion, and insertion mutationsDetection of genes for heat-stable enterotoxin I in Escherichia coli strains isolated in BrazilIdentification and classification of bacterial plasmids.Gene synthesis, expression, and mutagenesis of the blue copper proteins azurin and plastocyaninImportance of a hydrophobic residue in binding and catalysis by dihydrofolate reductase.Primary structure of the M subunit of the reaction center from Rhodopseudomonas sphaeroides.Active site of triosephosphate isomerase: in vitro mutagenesis and characterization of an altered enzyme.Two-codon insertion mutagenesis of plasmid genes by using single-stranded hexameric oligonucleotidesHigh-efficiency oligonucleotide-directed plasmid mutagenesis.Screening and separation of beta-lactam antibiotics using protein-engineered enzymes.Active-site serine mutants of the Streptomyces albus G beta-lactamase.
P2860
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P2860
Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function.
description
1982 nî lūn-bûn
@nan
1982年の論文
@ja
1982年学术文章
@wuu
1982年学术文章
@zh-cn
1982年学术文章
@zh-hans
1982年学术文章
@zh-my
1982年学术文章
@zh-sg
1982年學術文章
@yue
1982年學術文章
@zh
1982年學術文章
@zh-hant
name
Oligonucleotide-directed mutag ...... r studies of protein function.
@ast
Oligonucleotide-directed mutag ...... r studies of protein function.
@en
type
label
Oligonucleotide-directed mutag ...... r studies of protein function.
@ast
Oligonucleotide-directed mutag ...... r studies of protein function.
@en
prefLabel
Oligonucleotide-directed mutag ...... r studies of protein function.
@ast
Oligonucleotide-directed mutag ...... r studies of protein function.
@en
P2093
P2860
P356
P1476
Oligonucleotide-directed mutag ...... r studies of protein function.
@en
P2093
G Dalbadie-McFarland
J H Richards
P2860
P304
P356
10.1073/PNAS.79.21.6409
P407
P577
1982-11-01T00:00:00Z