Enhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screen
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Large-scale production and protein engineering of G protein-coupled receptors for structural studiesStructure determination of the seven-helix transmembrane receptor sensory rhodopsin II by solution NMR spectroscopyRecombinant human melatonin receptor MT1 isolated in mixed detergents shows pharmacology similar to that in mammalian cell membranesExpression and Subcellular Distribution of GFP-Tagged Human Tetraspanin Proteins in Saccharomyces cerevisiaeFunctional Stability of the Human Kappa Opioid Receptor Reconstituted in Nanodiscs Revealed by a Time-Resolved Scintillation Proximity AssayInvited review: GPCR structural characterization: Using fragments as building blocks to determine a complete structure.Tuning microbial hosts for membrane protein production.Isolation and pharmacological characterization of AdTx1, a natural peptide displaying specific insurmountable antagonism of the alpha1A-adrenoceptor.Single-cell profiling reveals heterogeneity and functional patterning of GPCR expression in the vascular system.The anti-hypertensive drug prazosin inhibits glioblastoma growth via the PKCδ-dependent inhibition of the AKT pathway.Expression and purification of functional human mu opioid receptor from E.coli.Optimisation of recombinant production of active human cardiac SERCA2a ATPaseOverexpression of membrane proteins from higher eukaryotes in yeasts.An artificial neural network for membrane-bound catechol-O-methyltransferase biosynthesis with Pichia pastoris methanol-induced cultures.High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae.Purification of recombinant G-protein-coupled receptors.Expression, purification and characterization of a phyA(m)-phyCs fusion phytase.Structural genomics and drug discovery.Using EGFP fusions to monitor the functional expression of GPCRs in the Drosophila Schneider 2 cells.Large-scale functional expression of WT and truncated human adenosine A2A receptor in Pichia pastoris bioreactor culturesGFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae.Relieving the first bottleneck in the drug discovery pipeline: using array technologies to rationalize membrane protein production.Advances in the production of membrane proteins in Pichia pastoris.High throughput platforms for structural genomics of integral membrane proteins.Methodological advances: the unsung heroes of the GPCR structural revolution.Single-cell profiling reveals GPCR heterogeneity and functional patterning during neuroinflammation.Recent advances in the production of recombinant subunit vaccines in Pichia pastoris.Identification of a novel protein-protein interaction motif mediating interaction of GPCR-associated sorting proteins with G protein-coupled receptors.Integral Membrane Protein Expression in Saccharomyces cerevisiae.Evaluation of the Pichia pastoris expression system for the production of GPCRs for structural analysis.Dimethylsulphoxide as a tool to increase functional expression of heterologously produced GPCRs in mammalian cells.Platform for the rapid construction and evaluation of GPCRs for crystallography in Saccharomyces cerevisiaeProgress toward heterologous expression of active G-protein-coupled receptors in Saccharomyces cerevisiae: Linking cellular stress response with translocation and trafficking.Comparative analysis and "expression space" coverage of the production of prokaryotic membrane proteins for structural genomics.Biological properties and perspective applications of "Bio-neuter" chemicals?Production of a recombinant membrane protein in an Escherichia coli strain for the whole cell biosynthesis of phenylacetic acids.Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yieldRecent Advances in the Application of Solution NMR Spectroscopy to Multi-Span Integral Membrane Proteins.Selective Assembly of Na,K-ATPase α2β2 Heterodimers in the Heart: DISTINCT FUNCTIONAL PROPERTIES AND ISOFORM-SELECTIVE INHIBITORS.Increasing cell biomass in Saccharomyces cerevisiae increases recombinant protein yield: the use of a respiratory strain as a microbial cell factory.
P2860
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P2860
Enhancing functional production of G protein-coupled receptors in Pichia pastoris to levels required for structural studies via a single expression screen
description
2006 nî lūn-bûn
@nan
2006年の論文
@ja
2006年学术文章
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2006年学术文章
@zh-cn
2006年学术文章
@zh-hans
2006年学术文章
@zh-my
2006年学术文章
@zh-sg
2006年學術文章
@yue
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@zh
2006年學術文章
@zh-hant
name
Enhancing functional productio ...... via a single expression screen
@ast
Enhancing functional productio ...... via a single expression screen
@en
type
label
Enhancing functional productio ...... via a single expression screen
@ast
Enhancing functional productio ...... via a single expression screen
@en
prefLabel
Enhancing functional productio ...... via a single expression screen
@ast
Enhancing functional productio ...... via a single expression screen
@en
P2093
P2860
P356
P1433
P1476
Enhancing functional productio ...... via a single expression screen
@en
P2093
Christoph Reinhart
Cécile Prual
Franc Pattus
Gabrielle Zeder-Lutz
Hartmut Michel
Nadia Cherouati
Nicolas André
Renaud Wagner
Tania Steffan
Thierry Magnin
P2860
P304
P356
10.1110/PS.062098206
P577
2006-04-05T00:00:00Z