Step-arrest mutants of FLP recombinase: implications for the catalytic mechanism of DNA recombination.
about
Requirements for catalysis in the Cre recombinase active siteFlexibility in DNA recombination: structure of the lambda integrase catalytic coreCleavage-dependent ligation by the FLP recombinase. Characterization of a mutant FLP protein with an alteration in a catalytic amino acidUnveiling hidden catalytic contributions of the conserved His/Trp-III in tyrosine recombinases: assembly of a novel active site in Flp recombinase harboring alanine at this positionViewing single lambda site-specific recombination events from start to finishMutations of the phage lambda attachment site alter the directionality of resolution of Holliday structures.Holliday junctions in FLP recombination: resolution by step-arrest mutants of FLP protein.Control of directionality in the DNA strand-exchange reaction catalysed by the tyrosine recombinase TnpI.Wild-type Flp recombinase cleaves DNA in trans.A novel recombinator in yeast based on gene II protein from bacteriophage f1Genetic analysis of the bacteriophage lambda attL nucleoprotein complex.Electrostatic suppression allows tyrosine site-specific recombination in the absence of a conserved catalytic arginineReversed DNA strand cleavage specificity in initiation of Cre-LoxP recombination induced by the His289Ala active-site substitutionRegulation of type 1 fimbriae by unlinked FimB- and FimE-like recombinases in uropathogenic Escherichia coli strain CFT073The integrase family of tyrosine recombinases: evolution of a conserved active site domainSimilarities and differences among 105 members of the Int family of site-specific recombinasesMechanism of active site exclusion in a site-specific recombinase: role of the DNA substrate in conferring half-of-the-sites activitySingle molecule TPM analysis of the catalytic pentad mutants of Cre and Flp site-specific recombinases: contributions of the pentad residues to the pre-chemical steps of recombinationMutagenesis of the IS1 transposase: importance of a His-Arg-Tyr triad for activity.Stereospecific suppression of active site mutants by methylphosphonate substituted substrates reveals the stereochemical course of site-specific DNA recombination.DNA recognition, strand selectivity, and cleavage mode during integrase family site-specific recombination.Functional analysis of box I mutations in yeast site-specific recombinases Flp and R: pairwise complementation with recombinase variants lacking the active-site tyrosine.Domain of a yeast site-specific recombinase (Flp) that recognizes its target siteLambda integrase cleaves DNA in cis.Functional roles of individual recombinase monomers in strand breakage and strand union during site-specific DNA recombination.Tn916 target DNA sequences bind the C-terminal domain of integrase protein with different affinities that correlate with transposon insertion frequency.Mechanism of cleavage and ligation by FLP recombinase: classification of mutations in FLP protein by in vitro complementation analysis.DNA sequence determinant for FIp-induced DNA bending.Excision of a conjugative transposon in vitro by the Int and Xis proteins of Tn916.Specific DNA cleavage mediated by the integrase of conjugative transposon Tn916.Expression of Flp Protein in a Baculovirus/Insect Cell System for Biotechnological Applications.Chemical probe and missing nucleoside analysis of Flp recombinase bound to the recombination target sequenceSynthesis of an enzymatically active FLP recombinase in vitro: search for a DNA-binding domainRecombinators, recombinases and recombination genes of yeasts.Conjugative transposition: Tn916 integrase contains two independent DNA binding domains that recognize different DNA sequencesSite-specific recombination in the replication terminus region of Escherichia coli: functional replacement of difXer site-specific recombination in vitroXer-mediated site-specific recombination in vitro.Active site electrostatics protect genome integrity by blocking abortive hydrolysis during DNA recombination.Identification of a potential general acid/base in the reversible phosphoryl transfer reactions catalyzed by tyrosine recombinases: Flp H305.
P2860
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P2860
Step-arrest mutants of FLP recombinase: implications for the catalytic mechanism of DNA recombination.
description
1988 nî lūn-bûn
@nan
1988年の論文
@ja
1988年論文
@yue
1988年論文
@zh-hant
1988年論文
@zh-hk
1988年論文
@zh-mo
1988年論文
@zh-tw
1988年论文
@wuu
1988年论文
@zh
1988年论文
@zh-cn
name
Step-arrest mutants of FLP rec ...... echanism of DNA recombination.
@ast
Step-arrest mutants of FLP rec ...... echanism of DNA recombination.
@en
type
label
Step-arrest mutants of FLP rec ...... echanism of DNA recombination.
@ast
Step-arrest mutants of FLP rec ...... echanism of DNA recombination.
@en
prefLabel
Step-arrest mutants of FLP rec ...... echanism of DNA recombination.
@ast
Step-arrest mutants of FLP rec ...... echanism of DNA recombination.
@en
P2093
P2860
P356
P1476
Step-arrest mutants of FLP rec ...... echanism of DNA recombination.
@en
P2093
P2860
P304
P356
10.1128/MCB.8.8.3303
P407
P577
1988-08-01T00:00:00Z