The pKa of the general acid/base carboxyl group of a glycosidase cycles during catalysis: a 13C-NMR study of bacillus circulans xylanase.
about
Cloning, mutagenesis, and structural analysis of human pancreatic alpha-amylase expressed in Pichia pastorisA historical perspective for the catalytic reaction mechanism of glycosidase; so as to bring about breakthrough in confusing situationCrystal structures of Geobacillus stearothermophilus alpha-glucuronidase complexed with its substrate and products: mechanistic implicationsStructural and enzymatic analysis of soybean beta-amylase mutants with increased pH optimumCrystal structure of Thermotoga maritima alpha-L-fucosidase. Insights into the catalytic mechanism and the molecular basis for fucosidosisAcid beta-glucosidase: insights from structural analysis and relevance to Gaucher disease therapyBacteria of the human gut microbiome catabolize red seaweed glycans with carbohydrate-active enzyme updates from extrinsic microbesX-ray crystallographic studies of family 11 xylanase Michaelis and product complexes: implications for the catalytic mechanismLigand Binding Enhances Millisecond Conformational Exchange in Xylanase B2 from Streptomyces lividansInsights into transition state stabilization of the beta-1,4-glycosidase Cex by covalent intermediate accumulation in active site mutantsLong-lived glycosyl-enzyme intermediate mimic produced by formate re-activation of a mutant endoglucanase lacking its catalytic nucleophilepH-Dependent conformational changes in proteins and their effect on experimental pK(a)s: the case of Nitrophorin 4Fungal Beta-glucosidases: a bottleneck in industrial use of lignocellulosic materialsThermal stability and kinetic constants for 129 variants of a family 1 glycoside hydrolase reveal that enzyme activity and stability can be separately designedEmpirical relationships between protein structure and carboxyl pKa values in proteins.Remeasuring HEWL pK(a) values by NMR spectroscopy: methods, analysis, accuracy, and implications for theoretical pK(a) calculations.Thermodynamic linkage between the binding of protons and inhibitors to HIV-1 protease.Uncovering the determinants of a highly perturbed tyrosine pKa in the active site of ketosteroid isomerase.From beta-glucanase to beta-glucansynthase: glycosyl transfer to alpha-glycosyl fluorides catalyzed by a mutant endoglucanase lacking its catalytic nucleophile.Complete measurement of the pKa values of the carboxyl and imidazole groups in Bacillus circulans xylanaseConformationally controlled pK-switching in membrane proteins: one more mechanism specific to the enzyme catalysis?NMR Determination of Protein pK(a) Values in the Solid StateDevelopment of inhibitors as research tools for carbohydrate-processing enzymes.Proton transfer facilitated by ligand binding. An energetic analysis of the catalytic mechanism of Trypanosoma cruzi trans-sialidase.Screening glycosynthase libraries with a fluoride chemosensor assay independently of enzyme specificity: identification of a transitional hydrolase to synthase mutant.Structural Insight into and Mutational Analysis of Family 11 Xylanases: Implications for Mechanisms of Higher pH Catalytic Adaptation.Rapid calculation of protein pKa values using Rosetta.Direct determination of protonation states and visualization of hydrogen bonding in a glycoside hydrolase with neutron crystallographyImprovement of alkalophilicity of an alkaline xylanase Xyn11A-LC from Bacillus sp. SN5 by random mutation and Glu135 saturation mutagenesis.Characterization of a buried neutral histidine residue in Bacillus circulans xylanase: NMR assignments, pH titration, and hydrogen exchange.On the evaluation and optimization of protein X-ray structures for pKa calculations.Calculating pKa values in enzyme active sites.Magnitude and origin of the enhanced basicity of the catalytic glutamate of triosephosphate isomeraseStructures of mammalian ER α-glucosidase II capture the binding modes of broad-spectrum iminosugar antivirals.Highly conserved histidine plays a dual catalytic role in protein splicing: a pKa shift mechanismAnalysis of site-specific histidine protonation in human prolactinGlycosidase inhibition: assessing mimicry of the transition state.Enzyme architecture: on the importance of being in a protein cage.Effect of acidic amino acids engineered into the active site cleft of Thermopolyspora flexuosa GH11 xylanase.pKa cycling of the general acid/base in glycoside hydrolase families 33 and 34.
P2860
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P2860
The pKa of the general acid/base carboxyl group of a glycosidase cycles during catalysis: a 13C-NMR study of bacillus circulans xylanase.
description
1996 nî lūn-bûn
@nan
1996年の論文
@ja
1996年論文
@yue
1996年論文
@zh-hant
1996年論文
@zh-hk
1996年論文
@zh-mo
1996年論文
@zh-tw
1996年论文
@wuu
1996年论文
@zh
1996年论文
@zh-cn
name
The pKa of the general acid/ba ...... f bacillus circulans xylanase.
@ast
The pKa of the general acid/ba ...... f bacillus circulans xylanase.
@en
type
label
The pKa of the general acid/ba ...... f bacillus circulans xylanase.
@ast
The pKa of the general acid/ba ...... f bacillus circulans xylanase.
@en
prefLabel
The pKa of the general acid/ba ...... f bacillus circulans xylanase.
@ast
The pKa of the general acid/ba ...... f bacillus circulans xylanase.
@en
P2093
P356
P1433
P1476
The pKa of the general acid/ba ...... f bacillus circulans xylanase.
@en
P2093
Johnson PE
McIntosh LP
Plesniak LA
Wakarchuk WW
Withers SG
P304
P356
10.1021/BI9613234
P407
P577
1996-08-01T00:00:00Z