Cre/lox system and PCR-based genome engineering in Bacillus subtilis.
about
A self-deleting Cre-lox-ermAM cassette, Cheshire, for marker-less gene deletion in Streptococcus pneumoniae.Current development in genetic engineering strategies of Bacillus species.Functional characterization and localization of a Bacillus subtilis sortase and its substrate and use of this sortase system to covalently anchor a heterologous protein to the B. subtilis cell wall for surface displayBacilysin overproduction in Bacillus amyloliquefaciens FZB42 markerless derivative strains FZBREP and FZBSPA enhances antibacterial activity.Bacillus subtilis as heterologous host for the secretory production of the non-ribosomal cyclodepsipeptide enniatin.Establishment of a markerless mutation delivery system in Bacillus subtilis stimulated by a double-strand break in the chromosome.MurJ and a novel lipid II flippase are required for cell wall biogenesis in Bacillus subtilis.Development of an efficient autoinducible expression system by promoter engineering in Bacillus subtilisConstruction and development of an auto-regulatory gene expression system in Bacillus subtilis.Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis.Electroporation-Based Genetic Manipulation in Type I Methanotrophs.Multigene disruption in undomesticated Bacillus subtilis ATCC 6051a using the CRISPR/Cas9 system.Simple and rapid direct cloning and heterologous expression of natural product biosynthetic gene cluster in Bacillus subtilis via Red/ET recombineering.A food-grade expression system for d-psicose 3-epimerase production in Bacillus subtilis using an alanine racemase-encoding selection marker.The molecular toolbox for chromosomal heterologous multiprotein expression in Escherichia coli.Developing Bacillus spp. as a cell factory for production of microbial enzymes and industrially important biochemicals in the context of systems and synthetic biology.Metabolic Engineering of Raoultella ornithinolytica BF60 for Production of 2,5-Furandicarboxylic Acid from 5-Hydroxymethylfurfural.A mobile genetic element profoundly increases heat resistance of bacterial spores.Cre-lox-based method for generation of large deletions within the genomic magnetosome island of Magnetospirillum gryphiswaldense.pheS * , an effective host-genotype-independent counter-selectable marker for marker-free chromosome deletion in Bacillus amyloliquefaciens.A transposon present in specific strains of Bacillus subtilis negatively affects nutrient- and dodecylamine-induced spore germination.Lysinibacillus fusiformis M5 induces increased complexity in Bacillus subtilis 168 colony biofilms via hypoxanthine.Genetic tool development for a new host for biotechnology, the thermotolerant bacterium Bacillus coagulans.A Novel Vector for Construction of Markerless Multicopy Overexpression Transformants in Pichia pastoris.Osmoprotection of Bacillus subtilis through import and proteolysis of proline-containing peptides.Bacillus subtilis genome editing using ssDNA with short homology regionsSite-specific recombination strategies for engineering actinomycete genomes.The γ-aminobutyrate permease GabP serves as the third proline transporter of Bacillus subtilis.Development of a CRISPR-Cas9 Tool Kit for Comprehensive Engineering of Bacillus subtilis.A high-efficiency recombineering system with PCR-based ssDNA in Bacillus subtilis mediated by the native phage recombinase GP35.Complex polar machinery required for proper chromosome segregation in vegetative and sporulating cells of Bacillus subtilisEngineering complex biological systems in bacteria through recombinase-assisted genome engineering.Establishment of a markerless multiple-gene deletion method based on Cre/loxP mutant system for Bacillus pumilus.Rewiring the Glucose Transportation and Central Metabolic Pathways for Overproduction of N-Acetylglucosamine in Bacillus subtilis.Overproduction of Rummeliibacillus pycnus arginase with multi-copy insertion of the arg R.pyc cassette into the Bacillus subtilis chromosome.Cre/lox-based multiple markerless gene disruption in the genome of the extreme thermophile Thermus thermophilus.Single-molecule live-cell imaging of bacterial DNA repair and damage tolerance.Effect of deletion of 2,3-butanediol dehydrogenase gene (bdhA) on acetoin production of Bacillus subtilis.An extracellular aminopeptidase encoded by the ywaD gene plays an important role in supplying nitrogen nutrition for the growth of Bacillus subtilis 168.Artificial induction of genetic competence in Bacillus amyloliquefaciens isolates.
P2860
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P2860
Cre/lox system and PCR-based genome engineering in Bacillus subtilis.
description
2008 nî lūn-bûn
@nan
2008年の論文
@ja
2008年論文
@yue
2008年論文
@zh-hant
2008年論文
@zh-hk
2008年論文
@zh-mo
2008年論文
@zh-tw
2008年论文
@wuu
2008年论文
@zh
2008年论文
@zh-cn
name
Cre/lox system and PCR-based genome engineering in Bacillus subtilis.
@en
type
label
Cre/lox system and PCR-based genome engineering in Bacillus subtilis.
@en
prefLabel
Cre/lox system and PCR-based genome engineering in Bacillus subtilis.
@en
P2093
P2860
P356
P1476
Cre/lox system and PCR-based genome engineering in Bacillus subtilis.
@en
P2093
P2860
P304
P356
10.1128/AEM.01156-08
P407
P577
2008-07-18T00:00:00Z