Structural and functional analysis of a replication enhancer: separation of the enhancer activity from origin function by mutational dissection of the replication origin gamma of plasmid R6K
about
Mechanistic aspects of DnaA-RepA interaction as revealed by yeast forward and reverse two-hybrid analysis.Investigations of pi initiator protein-mediated interaction between replication origins alpha and gamma of the plasmid R6K.Replication initiation at a distance: determination of the cis- and trans-acting elements of replication origin alpha of plasmid R6KTwo forms of replication initiator protein: positive and negative controls.Mechanistic studies of initiator-initiator interaction and replication initiation.AT-rich region and repeated sequences - the essential elements of replication origins of bacterial replicons.Initiator protein pi can bind independently to two domains of the gamma origin core of plasmid R6K: the direct repeats and the A+T-rich segmentMultiple pathways of copy control of gamma replicon of R6K: mechanisms both dependent on and independent of cooperativity of interaction of tau protein with DNA affect the copy number.Binding of DnaA protein to a replication enhancer counteracts the inhibition of plasmid R6K gamma origin replication mediated by elevated levels of R6K pi proteinCooperative binding mode of the inhibitors of R6K replication, pi dimers.The replication initiator protein pi of the plasmid R6K specifically interacts with the host-encoded helicase DnaB.The DnaK-DnaJ-GrpE chaperone system activates inert wild type pi initiator protein of R6K into a form active in replication initiation.Reconstitution of R6K DNA replication in vitro using 22 purified proteins.Replication and control of circular bacterial plasmids.Three clustered origins of replication in a promiscuous-plasmid replicon and their differential use in a PolA+ strain and a delta PolA strain of Escherichia coli K-12.
P2860
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P2860
Structural and functional analysis of a replication enhancer: separation of the enhancer activity from origin function by mutational dissection of the replication origin gamma of plasmid R6K
description
article científic
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article scientifique
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articolo scientifico
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artigo científico
@pt
bilimsel makale
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scientific article published on June 1992
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vedecký článok
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vetenskaplig artikel
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videnskabelig artikel
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vědecký článek
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name
Structural and functional anal ...... on origin gamma of plasmid R6K
@en
Structural and functional anal ...... n origin gamma of plasmid R6K.
@nl
type
label
Structural and functional anal ...... on origin gamma of plasmid R6K
@en
Structural and functional anal ...... n origin gamma of plasmid R6K.
@nl
prefLabel
Structural and functional anal ...... on origin gamma of plasmid R6K
@en
Structural and functional anal ...... n origin gamma of plasmid R6K.
@nl
P2093
P2860
P356
P1476
Structural and functional anal ...... on origin gamma of plasmid R6K
@en
P2093
P2860
P304
P356
10.1073/PNAS.89.11.5078
P407
P577
1992-06-01T00:00:00Z