Mass spectrometric determination of disulfide linkages in recombinant therapeutic proteins using online LC-MS with electron-transfer dissociation.
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Engineered single-domain antibodies with high protease resistance and thermal stabilityFragmentation follows structure: top-down mass spectrometry elucidates the topology of engineered cystine-knot miniproteinsLarge Scale Chemical Cross-linking Mass Spectrometry Perspectives.Integration of electrochemistry with ultra-performance liquid chromatography/mass spectrometryIdentification of disulfide bonds in protein proteolytic degradation products using de novo-protein unique sequence tags approachCharacterization of the glycosylation occupancy and the active site in the follow-on protein therapeutic: TNK-tissue plasminogen activatorTechniques for the analysis of cysteine sulfhydryls and oxidative protein folding.Charge-site-dependent dissociation of hydrogen-rich radical peptide cations upon vacuum UV photoexcitationDisulfide linkage engineering for improving biophysical properties of human VH domains.Advanced mass spectrometry workflows for analyzing disulfide bonds in biologics.Defining the disulfide bonds of insulin-like growth factor-binding protein-5 by tandem mass spectrometry with electron transfer dissociation and collision-induced dissociation.Simple approach to assign disulfide connectivity using extracted ion chromatograms of electron transfer dissociation spectra.Analytical tools for characterizing biopharmaceuticals and the implications for biosimilars.Carbon monoxide signaling in human red blood cells: evidence for pentose phosphate pathway activation and protein deglutathionylation.Antibody light chain variable domains and their biophysically improved versions for human immunotherapy.Therapeutic plasma proteins--application of proteomics in process optimization, validation, and analysis of the final product.High-resolution MS for structural characterization of protein therapeutics: advances and future directions.Strategies in mass spectrometry for the assignment of Cys-Cys disulfide connectivities in proteins.Qualitative and quantitative characterization of protein biotherapeutics with liquid chromatography mass spectrometry.Thiol-Disulfide Exchange in Human Growth Hormone.Identification of the unpaired cysteine status and complete mapping of the 17 disulfides of recombinant tissue plasminogen activator using LC-MS with electron transfer dissociation/collision induced dissociation.Mapping native disulfide bonds at a proteome scale.Electron transfer dissociation coupled to an Orbitrap analyzer may promise a straightforward and accurate sequencing of disulfide-bridged cyclic peptides: a case study.Characterization and comparison of disulfide linkages and scrambling patterns in therapeutic monoclonal antibodies: using LC-MS with electron transfer dissociation.Proteomic analysis and molecular modelling characterize the iron-regulatory protein haemojuvelin/repulsive guidance molecule cDesulfurization of cysteine-containing peptides resulting from sample preparation for protein characterization by mass spectrometry.Disulfide Bond Characterization of Endogenous IgG3 Monoclonal Antibodies Using LC-MS: An Investigation of IgG3 Disulfide-mediated Isoforms.Does Electron Capture Dissociation Cleave Protein Disulfide Bonds?Complete mapping of a cystine knot and nested disulfides of recombinant human arylsulfatase A by multi-enzyme digestion and LC-MS analysis using CID and ETD.Integration of online digestion and electrolytic reduction with mass spectrometry for rapid disulfide-containing protein structural analysis.The Role of Electron Transfer Dissociation in Modern Proteomics.Recent mass spectrometry-based techniques and considerations for disulfide bond characterization in proteins.Venom peptides as therapeutics: advances, challenges and the future of venom-peptide discovery.Effects of electron-transfer coupled with collision-induced dissociation (ET/CID) on doubly charged peptides and phosphopeptides.Direct mass spectrometric characterization of disulfide linkages.Mapping disulfide bonds from sub-micrograms of purified proteins or micrograms of complex protein mixtures.Exploring an Orbitrap Analyzer for the Characterization of Intact Antibodies by Native Mass Spectrometry
P2860
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P2860
Mass spectrometric determination of disulfide linkages in recombinant therapeutic proteins using online LC-MS with electron-transfer dissociation.
description
article científic
@ca
article scientifique
@fr
articolo scientifico
@it
artigo científico
@pt
bilimsel makale
@tr
scientific article published on January 2009
@en
vedecký článok
@sk
vetenskaplig artikel
@sv
videnskabelig artikel
@da
vědecký článek
@cs
name
Mass spectrometric determinati ...... lectron-transfer dissociation.
@en
Mass spectrometric determinati ...... lectron-transfer dissociation.
@nl
type
label
Mass spectrometric determinati ...... lectron-transfer dissociation.
@en
Mass spectrometric determinati ...... lectron-transfer dissociation.
@nl
prefLabel
Mass spectrometric determinati ...... lectron-transfer dissociation.
@en
Mass spectrometric determinati ...... lectron-transfer dissociation.
@nl
P2093
P2860
P356
P1433
P1476
Mass spectrometric determinati ...... lectron-transfer dissociation.
@en
P2093
Barry L Karger
Haitao Jiang
Qiaozhen Lu
Shiaw-Lin Wu
Shujia Dai
William S Hancock
P2860
P304
P356
10.1021/AC801560K
P407
P577
2009-01-01T00:00:00Z