Rapid and sensitive detection of Shiga toxin-producing Escherichia coli from nonenriched stool specimens by real-time PCR in comparison to enzyme immunoassay and culture.
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Detection, Characterization, and Typing of Shiga Toxin-Producing Escherichia coliA sensitive multiplex, real-time PCR assay for prospective detection of Shiga toxin-producing Escherichia coli from stool samples reveals similar incidences but variable severities of non-O157 and O157 infections in northern CaliforniaAttending rounds: microangiopathic hemolytic anemia with renal insufficiencyA qPCR assay to detect and quantify Shiga toxin-producing E. coli (STEC) in cattle and on farms: a potential predictive tool for STEC culture-positive farms.Inhibition controls for qualitative real-time PCR assays: are they necessary for all specimen matrices?Top-down proteomic identification of furin-cleaved α-subunit of Shiga toxin 2 from Escherichia coli O157:H7 using MALDI-TOF-TOF-MS/MS.Induction and identification of disulfide-intact and disulfide-reduced β-subunit of Shiga toxin 2 from Escherichia coli O157:H7 using MALDI-TOF-TOF-MS/MS and top-down proteomics.Three-hour molecular detection of Campylobacter, Salmonella, Yersinia, and Shigella species in feces with accuracy as high as that of culture.Development of a robust method for isolation of shiga toxin-positive Escherichia coli (STEC) from fecal, plant, soil and water samples from a leafy greens production region in California.Sensitive detection of Shiga Toxin 2 and some of its variants in environmental samples by a novel immuno-PCR assay.Multicenter evaluation of the BD max enteric bacterial panel PCR assay for rapid detection of Salmonella spp., Shigella spp., Campylobacter spp. (C. jejuni and C. coli), and Shiga toxin 1 and 2 genes.Simultaneous direct detection of Shiga-toxin producing Escherichia coli (STEC) strains by optical biosensing with oligonucleotide-functionalized gold nanoparticles.Prevalence of shiga toxin-producing Escherichia coli as detected by enzyme-linked immunoassays and real-time PCR during the summer months in northern Alberta, CanadaReal-Time PCR Assay for Detection and Differentiation of Shiga Toxin-Producing Escherichia coli from Clinical Samples.A Decade of Development of Chromogenic Culture Media for Clinical Microbiology in an Era of Molecular Diagnostics.Usability and performance of CHROMagar STEC medium in detection of Shiga toxin-producing Escherichia coli strainsFour-year experience with simultaneous culture and Shiga toxin testing for detection of Shiga toxin-producing Escherichia coli in stool samples.Changing Diagnostic Methods and Increased Detection of Verotoxigenic Escherichia coli, Ireland.Implications of free Shiga toxin-converting bacteriophages occurring outside bacteria for the evolution and the detection of Shiga toxin-producing Escherichia coli.Bacteriophages carrying Shiga toxin genes: genomic variations, detection and potential treatment of pathogenic bacteria.Real-time PCR as a diagnostic tool for bacterial diseases.Two Laboratory Activities Using Conventional or Real-Time PCR to Simulate Pathogenic E. coli DetectionSuitability of stx-PCR directly from fecal samples in clinical diagnostics of STEC.Shiga toxin 2-encoding bacteriophages in human fecal samples from healthy individuals.Using Bioinformatics to Develop and Test Hypotheses: E. coli-Specific Virulence Determinants.Improving detection of Shiga toxin-producing Escherichia coli by molecular methods by reducing the interference of free Shiga toxin-encoding bacteriophages.Multiplex real-time PCR assay for detection of Escherichia coli O157:H7 and screening for non-O157 Shiga toxin-producing E. coli.Development of a real-time PCR assay and comparison to CHROMagarTM STEC to screen for Shiga toxin-producing Escherichia coli in stool, Cape Town, South Africa.
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P2860
Rapid and sensitive detection of Shiga toxin-producing Escherichia coli from nonenriched stool specimens by real-time PCR in comparison to enzyme immunoassay and culture.
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article científic
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article scientifique
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articolo scientifico
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artigo científico
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bilimsel makale
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scientific article published on 13 May 2009
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vedecký článok
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vetenskaplig artikel
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videnskabelig artikel
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vědecký článek
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name
Rapid and sensitive detection ...... nzyme immunoassay and culture.
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Rapid and sensitive detection ...... nzyme immunoassay and culture.
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type
label
Rapid and sensitive detection ...... nzyme immunoassay and culture.
@en
Rapid and sensitive detection ...... nzyme immunoassay and culture.
@nl
prefLabel
Rapid and sensitive detection ...... nzyme immunoassay and culture.
@en
Rapid and sensitive detection ...... nzyme immunoassay and culture.
@nl
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P2860
P356
P1476
Rapid and sensitive detection ...... nzyme immunoassay and culture.
@en
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Jon E Rosenblatt
Lynne M Sloan
Robin Patel
Thomas E Grys
P2860
P304
P356
10.1128/JCM.02013-08
P407
P577
2009-05-13T00:00:00Z