Reaction mechanisms of thiamin diphosphate enzymes: defining states of ionization and tautomerization of the cofactor at individual steps.
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Structural Insights into the Prereaction State of Pyruvate Decarboxylase from Zymomonas mobilis ,Communication between Thiamin Cofactors in the Escherichia coli Pyruvate Dehydrogenase Complex E1 Component Active Centers: EVIDENCE FOR A"DIRECT PATHWAY"BETWEEN THE 4'-AMINOPYRIMIDINE N1' ATOMSDouble Duty for a Conserved Glutamate in Pyruvate Decarboxylase: Evidence of the Participation in Stereoelectronically Controlled Decarboxylation and in Protonation of the Nascent Carbanion/Enamine Intermediate,Unexpected tautomeric equilibria of the carbanion-enamine intermediate in pyruvate oxidase highlight unrecognized chemical versatility of thiaminStructure and Function of the Catalytic Domain of the Dihydrolipoyl Acetyltransferase Component in Escherichia coli Pyruvate Dehydrogenase ComplexObservation of a stable carbene at the active site of a thiamin enzymeA dual conformation of the post-decarboxylation intermediate is associated with distinct enzyme states in mycobacterial KGD (α-ketoglutarate decarboxylase)A δ38 deletion variant of human transketolase as a model of transketolase-like protein 1 exhibits no enzymatic activityCyclohexane-1,2-dione hydrolase from denitrifying Azoarcus sp. strain 22Lin, a novel member of the thiamine diphosphate enzyme familyNuclear magnetic resonance evidence for the role of the flexible regions of the E1 component of the pyruvate dehydrogenase complex from gram-negative bacteria.The pyruvate dehydrogenase complexes: structure-based function and regulation.Structure and functional characterization of pyruvate decarboxylase from Gluconacetobacter diazotrophicus.Direct observation of ground-state lactam-lactim tautomerization using temperature-jump transient 2D IR spectroscopy.Assignment of function to histidines 260 and 298 by engineering the E1 component of the Escherichia coli 2-oxoglutarate dehydrogenase complex; substitutions that lead to acceptance of substrates lacking the 5-carboxyl groupExperimental observation of thiamin diphosphate-bound intermediates on enzymes and mechanistic information derived from these observationsCompetence of Thiamin Diphosphate-Dependent Enzymes with 2'-Methoxythiamin Diphosphate Derived from Bacimethrin, a Naturally Occurring Thiamin Anti-vitaminUsing site-saturation mutagenesis to explore mechanism and substrate specificity in thiamin diphosphate-dependent enzymes.Identification of charge transfer transitions related to thiamin-bound intermediates on enzymes provides a plethora of signatures useful in mechanistic studies.Observation of thiamin-bound intermediates and microscopic rate constants for their interconversion on 1-deoxy-D-xylulose 5-phosphate synthase: 600-fold rate acceleration of pyruvate decarboxylation by D-glyceraldehyde-3-phosphate.Glyoxylate carboligase: a unique thiamin diphosphate-dependent enzyme that can cycle between the 4'-aminopyrimidinium and 1',4'-iminopyrimidine tautomeric forms in the absence of the conserved glutamateThiamin Diphosphate Activation in 1-Deoxy-d-xylulose 5-Phosphate Synthase: Insights into the Mechanism and Underlying Intermolecular InteractionsBifunctionality of the thiamin diphosphate cofactor: assignment of tautomeric/ionization states of the 4'-aminopyrimidine ring when various intermediates occupy the active sites during the catalysis of yeast pyruvate decarboxylase.Identification of Lactam-Lactim Tautomers of Aromatic Heterocycles in Aqueous Solution Using 2D IR Spectroscopy.Crystal structure of a ring-cleaving cyclohexane-1,2-dione hydrolase, a novel member of the thiamine diphosphate enzyme family.Gold(I)-catalysed synthesis of a furan analogue of thiamine pyrophosphate.Electron density reactivity indexes of the tautomeric/ionization forms of thiamin diphosphate.
P2860
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P2860
Reaction mechanisms of thiamin diphosphate enzymes: defining states of ionization and tautomerization of the cofactor at individual steps.
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article científic
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article scientifique
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articolo scientifico
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artigo científico
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bilimsel makale
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scientific article published on 16 March 2009
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vedecký článok
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vetenskaplig artikel
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videnskabelig artikel
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vědecký článek
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name
Reaction mechanisms of thiamin ...... cofactor at individual steps.
@en
Reaction mechanisms of thiamin ...... cofactor at individual steps.
@nl
type
label
Reaction mechanisms of thiamin ...... cofactor at individual steps.
@en
Reaction mechanisms of thiamin ...... cofactor at individual steps.
@nl
prefLabel
Reaction mechanisms of thiamin ...... cofactor at individual steps.
@en
Reaction mechanisms of thiamin ...... cofactor at individual steps.
@nl
P2093
P2860
P1433
P1476
Reaction mechanisms of thiamin ...... e cofactor at individual steps
@en
P2093
Frank Jordan
Natalia S Nemeria
Sumit Chakraborty
P2860
P304
P356
10.1111/J.1742-4658.2009.06964.X
P407
P577
2009-03-16T00:00:00Z