Identification of glutamic acid 78 as the active site nucleophile in Bacillus subtilis xylanase using electrospray tandem mass spectrometry.
about
Structural and enzymatic analysis of soybean beta-amylase mutants with increased pH optimumX-ray crystallographic studies of family 11 xylanase Michaelis and product complexes: implications for the catalytic mechanismCrystal structure of the family 7 endoglucanase I (Cel7B) from Humicola insolens at 2.2 A resolution and identification of the catalytic nucleophile by trapping of the covalent glycosyl-enzyme intermediateIdentification of Glu-330 as the catalytic nucleophile of Candida albicans exo-beta-(1,3)-glucanaseLong-lived glycosyl-enzyme intermediate mimic produced by formate re-activation of a mutant endoglucanase lacking its catalytic nucleophileTransglycosylation: a mechanism for RNA modification (and editing?).Approaches to labeling and identification of active site residues in glycosidases.Characterization of a buried neutral histidine residue in Bacillus circulans xylanase: NMR assignments, pH titration, and hydrogen exchange.Secondary structure and NMR assignments of Bacillus circulans xylanaseProbing the role of sigma π interaction and energetics in the catalytic efficiency of endo-1,4-β-xylanaseActive-site peptide "fingerprinting" of glycosidases in complex mixtures by mass spectrometry. Discovery of a novel retaining beta-1,4-glycanase in Cellulomonas fimi.Identification of glu-277 as the catalytic nucleophile of Thermoanaerobacterium saccharolyticum beta-xylosidase using electrospray MS.Substrate-binding site of family 11 xylanase from Bacillus firmus K-1 by molecular docking.Rationalising pKa shifts in Bacillus circulans xylanase with computational studies.Synthesis and testing of mechanism-based protein-profiling probes for retaining endo-glycosidases.Use of Fluorinated Functionality in Enzyme Inhibitor Development: Mechanistic and Analytical AdvantagesIdentification of Glu-120 as the catalytic nucleophile in Streptomyces lividans endoglucanase celB.Analysis of the dynamic properties of Bacillus circulans xylanase upon formation of a covalent glycosyl-enzyme intermediateSalicortin: a repeat-attack new-mechanism-based Agrobacterium faecalis beta-glucosidase inhibitor.Structure-function relationship of xylanase: fluorimetric analysis of the tryptophan environment.Identification of Glu-519 as the catalytic nucleophile in beta-mannosidase 2A from Cellulomonas fimi.Improving the alkalophilic performances of the Xyl1 xylanase from Streptomyces sp. S38: structural comparison and mutational analysis.Computational mutagenesis reveals the role of active-site tyrosine in stabilising a boat conformation for the substrate: QM/MM molecular dynamics studies of wild-type and mutant xylanases.
P2860
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P2860
Identification of glutamic acid 78 as the active site nucleophile in Bacillus subtilis xylanase using electrospray tandem mass spectrometry.
description
1994 nî lūn-bûn
@nan
1994年の論文
@ja
1994年論文
@yue
1994年論文
@zh-hant
1994年論文
@zh-hk
1994年論文
@zh-mo
1994年論文
@zh-tw
1994年论文
@wuu
1994年论文
@zh
1994年论文
@zh-cn
name
Identification of glutamic aci ...... pray tandem mass spectrometry.
@en
type
label
Identification of glutamic aci ...... pray tandem mass spectrometry.
@en
prefLabel
Identification of glutamic aci ...... pray tandem mass spectrometry.
@en
P2093
P356
P1433
P1476
Identification of glutamic aci ...... pray tandem mass spectrometry.
@en
P2093
P304
P356
10.1021/BI00189A002
P407
P577
1994-06-01T00:00:00Z