Enhanced expression, native purification, and characterization of CCR5, a principal HIV-1 coreceptor
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Structure-Based Design, Synthesis, and Characterization of Dual Hotspot Small-Molecule HIV-1 Entry InhibitorsJM4 is a four-transmembrane protein binding to the CCR5 receptorHuman alpha1-acid glycoprotein binds to CCR5 expressed on the plasma membrane of human primary macrophagesHigh-level production, solubilization and purification of synthetic human GPCR chemokine receptors CCR5, CCR3, CXCR4 and CX3CR1Broadly cross-reactive HIV-1-neutralizing human monoclonal Fab selected for binding to gp120-CD4-CCR5 complexes.Study of a synthetic human olfactory receptor 17-4: expression and purification from an inducible mammalian cell line.Paramagnetic proteoliposomes containing a pure, native, and oriented seven-transmembrane segment protein, CCR5.An antibody library for stabilizing and crystallizing membrane proteins - selecting binders to the citrate carrier CitS.Identification of a linear peptide recognized by monoclonal antibody 2D7 capable of generating CCR5-specific antibodies with human immunodeficiency virus-neutralizing activity.Modifications that stabilize human immunodeficiency virus envelope glycoprotein trimers in solution.The level of CD4 expression limits infection of primary rhesus monkey macrophages by a T-tropic simian immunodeficiency virus and macrophagetropic human immunodeficiency viruses.CD4-independent use of Rhesus CCR5 by human immunodeficiency virus Type 2 implicates an electrostatic interaction between the CCR5 N terminus and the gp120 C4 domain.Increased neutralization sensitivity of CD4-independent human immunodeficiency virus variantsStructure-based identification and neutralization mechanism of tyrosine sulfate mimetics that inhibit HIV-1 entry.Increased CCR5 affinity and reduced CCR5/CD4 dependence of a neurovirulent primary human immunodeficiency virus type 1 isolate.Identification of conserved and variable structures in the human immunodeficiency virus gp120 glycoprotein of importance for CXCR4 bindingRelating surfactant properties to activity and solubilization of the human adenosine a3 receptorDirect measurement of thermal stability of expressed CCR5 and stabilization by small molecule ligands.Design, synthesis and biological evaluation of small molecule inhibitors of CD4-gp120 binding based on virtual screeningThe isolation of novel phage display-derived human recombinant antibodies against CCR5, the major co-receptor of HIVHighly stable trimers formed by human immunodeficiency virus type 1 envelope glycoproteins fused with the trimeric motif of T4 bacteriophage fibritin.A rapid and efficient way to obtain modified chemokines for functional and biophysical studiesImmunization of rabbits with highly purified, soluble, trimeric human immunodeficiency virus type 1 envelope glycoprotein induces a vigorous B cell response and broadly cross-reactive neutralization.Identification of Owl Monkey CD4 Receptors Broadly Compatible with Early-Stage HIV-1 IsolatesChemokine receptor internalization and intracellular trafficking.Sialylated O-glycans and sulfated tyrosines in the NH2-terminal domain of CC chemokine receptor 5 contribute to high affinity binding of chemokinesA species-specific amino acid difference in the macaque CD4 receptor restricts replication by global circulating HIV-1 variants representing viruses from recent infection.Antigenic conservation and immunogenicity of the HIV coreceptor binding siteCharacterization of the steric defense of the HIV-1 gp41 N-trimer region.Localized changes in the gp120 envelope glycoprotein confer resistance to human immunodeficiency virus entry inhibitors BMS-806 and #155.Adenovirus mediated expression "in vivo" of the chemokine receptor CXCR1Expression, surface immobilization, and characterization of functional recombinant cannabinoid receptor CB2Factors that increase the effective concentration of CXCR4 dictate feline immunodeficiency virus tropism and kinetics of replication.Amino acids 270 to 510 of the severe acute respiratory syndrome coronavirus spike protein are required for interaction with receptor.Retroviruses pseudotyped with the severe acute respiratory syndrome coronavirus spike protein efficiently infect cells expressing angiotensin-converting enzyme 2.Simian Immunodeficiency Virus SIVmac239, but Not SIVmac316, Binds and Utilizes Human CD4 More Efficiently than Rhesus CD4.Palmitoylation of CCR5 is critical for receptor trafficking and efficient activation of intracellular signaling pathways.Endocytosis and recycling of the HIV coreceptor CCR5Efficient expression screening of human membrane proteins in transiently transfected Human Embryonic Kidney 293S cells.Optimizing heterologous expression in dictyostelium: importance of 5' codon adaptation.
P2860
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P2860
Enhanced expression, native purification, and characterization of CCR5, a principal HIV-1 coreceptor
description
1999 nî lūn-bûn
@nan
1999年の論文
@ja
1999年学术文章
@wuu
1999年学术文章
@zh-cn
1999年学术文章
@zh-hans
1999年学术文章
@zh-my
1999年学术文章
@zh-sg
1999年學術文章
@yue
1999年學術文章
@zh
1999年學術文章
@zh-hant
name
Enhanced expression, native pu ...... , a principal HIV-1 coreceptor
@en
type
label
Enhanced expression, native pu ...... , a principal HIV-1 coreceptor
@en
prefLabel
Enhanced expression, native pu ...... , a principal HIV-1 coreceptor
@en
P2093
P2860
P356
P1476
Enhanced expression, native pu ...... , a principal HIV-1 coreceptor
@en
P2093
P2860
P304
28745-28750
P356
10.1074/JBC.274.40.28745
P407
P577
1999-10-01T00:00:00Z