Recombinant enterokinase light chain with affinity tag: expression from Saccharomyces cerevisiae and its utilities in fusion protein technology.
about
Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: the novel Fh8 systemAtomic resolution structure of a protein prepared by non-enzymatic His-tag removal. Crystallographic and NMR study of GmSPI-2 inhibitor.Enhancing the specificity of the enterokinase cleavage reaction to promote efficient cleavage of a fusion tagRecombinant protein expression and purification: a comprehensive review of affinity tags and microbial applications.The variable detergent sensitivity of proteases that are utilized for recombinant protein affinity tag removal.An overview of enzymatic reagents for the removal of affinity tags.Several affinity tags commonly used in chromatographic purification.Strategies to optimize protein expression in E. coli.Exploiting the interactions between poly-histidine fusion tags and immobilized metal ions.Current strategies for protein production and purification enabling membrane protein structural biology.Production and purification of recombinant enteropeptidase expressed in an insect-baculovirus cell system.Human enteropeptidase light chain: bioengineering of recombinants and kinetic investigations of structure and functionAn expression and purification system for the biosynthesis of adenosine receptor peptides for biophysical and structural characterization.Surface supercharged human enteropeptidase light chain shows improved solubility and refolding yield.Mn²⁺/Mg ²⁺-dependent pyruvate kinase from a D-lactic acid-producing bacterium Sporolactobacillus inulinus: characterization of a novel Mn²⁺-mediated allosterically regulated enzyme.Design and efficient production of bovine enterokinase light chain with higher specificity in E. coli.
P2860
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P2860
Recombinant enterokinase light chain with affinity tag: expression from Saccharomyces cerevisiae and its utilities in fusion protein technology.
description
2001 nî lūn-bûn
@nan
2001年の論文
@ja
2001年論文
@yue
2001年論文
@zh-hant
2001年論文
@zh-hk
2001年論文
@zh-mo
2001年論文
@zh-tw
2001年论文
@wuu
2001年论文
@zh
2001年论文
@zh-cn
name
Recombinant enterokinase light ...... in fusion protein technology.
@en
type
label
Recombinant enterokinase light ...... in fusion protein technology.
@en
prefLabel
Recombinant enterokinase light ...... in fusion protein technology.
@en
P2093
P2860
P356
P1476
Recombinant enterokinase light ...... in fusion protein technology.
@en
P2093
P2860
P304
P356
10.1002/BIT.10082
P577
2001-12-01T00:00:00Z