A high-yielding CHO transient system: coexpression of genes encoding EBNA-1 and GS enhances transient protein expression.
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Structures of closed and open conformations of dimeric human ATMSynthetic promoters for CHO cell engineering.Enhanced delivery of IL-1 receptor antagonist to the central nervous system as a novel anti-transferrin receptor-IL-1RA fusion reverses neuropathic mechanical hypersensitivity.New Mammalian Expression Systems.Polysome profiling of mAb producing CHO cell lines links translational control of cell proliferation and recombinant mRNA loading onto ribosomes with global and recombinant protein synthesis.Use of a protein engineering strategy to overcome limitations in the production of "Difficult to Express" recombinant proteins.Optimized production of HIV-1 virus-like particles by transient transfection in CAP-T cells.Deletion of a telomeric region on chromosome 8 correlates with higher productivity and stability of CHO cell lines.Transcriptional and post-transcriptional targeting for enhanced transient gene expression in CHO cells.CHO-S antibody titers >1 gram/liter using flow electroporation-mediated transient gene expression followed by rapid migration to high-yield stable cell lines.A high cell density transient transfection system for therapeutic protein expression based on a CHO GS-knockout cell line: process development and product quality assessment.High-throughput mAb expression and purification platform based on transient CHO.Effects of glutamine and asparagine on recombinant antibody production using CHO-GS cell lines.Non-conventional expression systems for the production of vaccine proteins and immunotherapeutic molecules.Engineering the surface properties of a human monoclonal antibody prevents self-association and rapid clearance in vivo.Immunization with Low Doses of Recombinant Postfusion or Prefusion Respiratory Syncytial Virus F Primes for Vaccine-Enhanced Disease in the Cotton Rat Model Independently of the Presence of a Th1-Biasing (GLA-SE) or Th2-Biasing (Alum) Adjuvant.Using Gene Essentiality and Synthetic Lethality Information to Correct Yeast and CHO Cell Genome-Scale Models.Structural and functional characterization of a specific antidote for ticagrelor.A simple, rapid method for evaluation of transfection efficiency based on fluorescent dye.A fast and easy strategy for protein purification using "teabags".NF-κB, CRE and YY1 elements are key functional regulators of CMV promoter-driven transient gene expression in CHO cells.The TGF-β inhibitory activity of antibody 37E1B5 depends on its H-CDR2 glycan.A CD80-Biased CTLA4-Ig Fusion Protein with Superior In Vivo Efficacy by Simultaneous Engineering of Affinity, Selectivity, Stability, and FcRn Binding.Exploring cellular behavior under transient gene expression and its impact on mAb productivity and Fc-glycosylation.Polymer-mediated flocculation of transient CHO cultures as a simple, high throughput method to facilitate antibody discovery.Advancements in mammalian cell transient gene expression (TGE) technology for accelerated production of biologics.Central inhibition of granulocyte-macrophage colony-stimulating factor is analgesic in experimental neuropathic pain.Generation of stable Chinese hamster ovary pools yielding antibody titers of up to 7.6 g/L using the piggyBac transposon system.Engineering the expression of an anti-interleukin-13 antibody through rational design and mutagenesis.N-terminal or signal peptide sequence engineering prevents truncation of human monoclonal antibody light chains.Structure and biophysical characterisation of the human full-length Neurturin-GFRa2 complex - a role for heparan sulfate in signalling.Enhanced plasmid DNA utilization in transiently transfected CHO-DG44 cells in the presence of polar solvents.Large-Scale Transient Transfection of Chinese Hamster Ovary Cells in Suspension.Comparison of three transposons for the generation of highly productive recombinant CHO cell pools and cell lines.Integrated cell and process engineering for improved transient production of a "difficult-to-express" fusion protein by CHO cells.Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells.A mechanistic dissection of polyethylenimine mediated transfection of CHO cells: to enhance the efficiency of recombinant DNA utilization.Isolation of blood-brain barrier-crossing antibodies from a phage display library by competitive elution and their ability to penetrate the central nervous system.Intracellular sorting and transcytosis of the rat transferrin receptor antibody OX26 across the blood-brain barrier in vitro is dependent on its binding affinity
P2860
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P2860
A high-yielding CHO transient system: coexpression of genes encoding EBNA-1 and GS enhances transient protein expression.
description
2013 nî lūn-bûn
@nan
2013年の論文
@ja
2013年論文
@yue
2013年論文
@zh-hant
2013年論文
@zh-hk
2013年論文
@zh-mo
2013年論文
@zh-tw
2013年论文
@wuu
2013年论文
@zh
2013年论文
@zh-cn
name
A high-yielding CHO transient ...... transient protein expression.
@en
A high-yielding CHO transient ...... transient protein expression.
@nl
type
label
A high-yielding CHO transient ...... transient protein expression.
@en
A high-yielding CHO transient ...... transient protein expression.
@nl
prefLabel
A high-yielding CHO transient ...... transient protein expression.
@en
A high-yielding CHO transient ...... transient protein expression.
@nl
P2093
P2860
P356
P1476
A high-yielding CHO transient ...... transient protein expression.
@en
P2093
Diane Hatton
Gary Pettman
Jessica Stevenson
Olalekan Daramola
William Holmes
P2860
P304
P356
10.1002/BTPR.1809
P577
2013-10-10T00:00:00Z