Large-scale purification and characterization of dihydrofolate reductase from a methotrexate-resistant strain of Lactobacillus casei.
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Structure and dynamics in solution of the complex of lactobacillus casei dihydrofolate reductase with the new lipophilic antifolate drug trimetrexateDiscovery of potent pteridine reductase inhibitors to guide antiparasite drug developmentCrystal Structure of Bacillus anthracis Dihydrofolate Reductase with the Dihydrophthalazine-Based Trimethoprim Derivative RAB1 Provides a Structural Explanation of Potency and SelectivityKinetic and Structural Characterization of Dihydrofolate Reductase from Streptococcus pneumoniaeNMR Structures of Apo L. casei Dihydrofolate Reductase and Its Complexes with Trimethoprim and NADPH: Contributions to Positive Cooperative Binding from Ligand-Induced Refolding, Conformational Changes, and Interligand Hydrophobic InteractionsMoritella cold-active dihydrofolate reductase: are there natural limits to optimization of catalytic efficiency at low temperature?Dynamics of trimethoprim bound to dihydrofolate reductase.A 1H n.m.r. study of the role of the glutamate moiety in the binding of methotrexate to Lactobacillus casei dihydrofolate reductasePeroxidase catalysed aerobic degradation of 5,6,7,8-tetrahydrobiopterin at physiological pH.31P solid-state NMR measurements used to detect interactions between NADPH and water and to determine the ionisation state of NADPH in a protein-ligand complex subjected to low-level hydration.NMR detection of arginine-ligand interactions in complexes of Lactobacillus casei dihydrofolate reductase.Biochemical characterization of a dihydromethanopterin reductase involved in tetrahydromethanopterin biosynthesis in Methylobacterium extorquens AM1.Crystallization and preliminary crystallographic studies of dihydrofolate reductase-thymidylate synthase from Trypanosoma cruzi, the Chagas disease pathogen.31P NMR studies of the binding of adenosine-2'-phosphate to Lactobacillus casei dihydrofolate reductase.Ultraviolet difference-spectroscopic studies of substrate and inhibitor binding to Lactobacillus casei dihydrofolate reductase.Circular-dichroism studies of ligand binding to dihydrofolate reductase from Lactobacillus casei MTX/RInteractions between inhibitors of dihydrofolate reductase.3H-n.m.r. studies of multiple conformations and dynamic processes in complexes of folate and methotrexate with Lactobacillus casei dihydrofolate reductaseDirect 19F NMR observation of the conformational selection of optically active rotamers of the antifolate compound fluoronitropyrimethamine bound to the enzyme dihydrofolate reductase.Purification and characterization of dihydrofolate reductase from Mycobacterium phlei.19F-n.m.r. studies of 3',5'-difluoromethotrexate binding to Lactobacillus casei dihydrofolate reductase. Molecular motion and coenzyme-induced conformational changes.The effects of modification with N-bromosuccinimide on the binding of ligands to dihydrofolate reductase.Cloning and characterization of dihydrofolate reductase from a facultative alkaliphilic and halotolerant bacillus strain.High expression in Escherichia coli of the gene coding for dihydrofolate reductase of the extremely halophilic archaebacterium Haloferax volcanii. Reconstitution of the active enzyme and mutation studies.A novel method of preparing totally alpha-deuterated amino acids for selective incorporation into proteins. Application to assignment of 1H resonances of valine residues in dihydrofolate reductase.On the use of PIPSA to guide target-selective drug design.Modulation of specific binding of Lactobacillus casei dihydrofolate reductase to DNA by folinic acid15 N and 1 H NMR evidence for multiple conformations of the complex of dihydrofolate reductase with its substrate, folateIdentification of the 1 H resonances of valine and leucine residues in dihydrofolate reductase by using a combination of selective deuteration and two-dimensional correlation spectroscopyThe combined use of selective deuteration and double resonance experiments in assigning the 1 H resonances of valine and tyrosine residues of dihydrofolate reductaseThe 1H-NMR assignments of the aromatic resonances in complexes of Lactobacillus casei dihydrofolate reductase and the origins of their chemical shifts
P2860
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P2860
Large-scale purification and characterization of dihydrofolate reductase from a methotrexate-resistant strain of Lactobacillus casei.
description
1976 nî lūn-bûn
@nan
1976年の論文
@ja
1976年論文
@yue
1976年論文
@zh-hant
1976年論文
@zh-hk
1976年論文
@zh-mo
1976年論文
@zh-tw
1976年论文
@wuu
1976年论文
@zh
1976年论文
@zh-cn
name
Large-scale purification and c ...... strain of Lactobacillus casei.
@en
Large-scale purification and c ...... strain of Lactobacillus casei.
@nl
type
label
Large-scale purification and c ...... strain of Lactobacillus casei.
@en
Large-scale purification and c ...... strain of Lactobacillus casei.
@nl
prefLabel
Large-scale purification and c ...... strain of Lactobacillus casei.
@en
Large-scale purification and c ...... strain of Lactobacillus casei.
@nl
P2093
P2860
P356
P1433
P1476
Large-scale purification and c ...... strain of Lactobacillus casei.
@en
P2093
Roberts GC
P2860
P304
P356
10.1042/BJ1570559
P407
P577
1976-09-01T00:00:00Z