about
Crystal and electron microscopy structures of sticholysin II actinoporin reveal insights into the mechanism of membrane pore formation1H, 13C, and 15N NMR assignments of the actinoporin Sticholysin IIntrinsic local disorder and a network of charge-charge interactions are key to actinoporin membrane disruption and cytotoxicityNMR solution structure of the antifungal protein from Aspergillus giganteus: evidence for cysteine pairing isomerismSpecific interactions of sticholysin I with model membranes: an NMR study.Thermal unfolding of the cytotoxin alpha-sarcin: phospholipid binding induces destabilization of the protein structure.Influence of key residues on the heterologous extracellular production of fungal ribonuclease U2 in the yeast Pichia pastoris.Cleavage of the sarcin-ricin loop of 23S rRNA differentially affects EF-G and EF-Tu bindingSecretion of recombinant pro- and mature fungal alpha-sarcin ribotoxin by the methylotrophic yeast Pichia pastoris: the Lys-Arg motif is required for maturation.Three-dimensional structure of the actinoporin sticholysin I. Influence of long-distance effects on protein function.Fungal ribotoxins: Natural protein-based weapons against insects.Involvement of the amino-terminal beta-hairpin of the Aspergillus ribotoxins on the interaction with membranes and nonspecific ribonuclease activity.Involvement of loops 2 and 3 of α-sarcin on its ribotoxic activity.α-sarcin and RNase T1 based immunoconjugates: the role of intracellular trafficking in cytotoxic efficiency.A non-cytotoxic but ribonucleolytically specific ribotoxin variant: implication of tryptophan residues in the cytotoxicity of hirsutellin A.Efficient in vivo antitumor effect of an immunotoxin based on ribotoxin α-sarcin in nude mice bearing human colorectal cancer xenografts.The role of electrostatic interactions in the antitumor activity of dimeric RNases.Production and characterization of a noncytotoxic deletion variant of the Aspergillus fumigatus allergen Aspf1 displaying reduced IgE binding.On the degradation of intracellular RNA by ribonucleases.Establishment and characterization of a new human colon adenocarcinoma cell line: BCS-TC2.Leucine 145 of the ribotoxin alpha-sarcin plays a key role for determining the specificity of the ribosome-inactivating activity of the protein.Fungal extracellular ribotoxins as insecticidal agents.The effect of cholesterol on the long-range network of interactions established among sea anemone Sticholysin II residues at the water-membrane interfaceNMR structure of the noncytotoxic alpha-sarcin mutant Delta(7-22): the importance of the native conformation of peripheral loops for activity.Production of the biotechnologically relevant AFP from Aspergillus giganteus in the yeast Pichia pastoris.Increased electrophoretic mobility of sodium sulfite-treated jack bean urease.Cytotoxic mechanism of the ribotoxin alpha-sarcin. Induction of cell death via apoptosis.Arginine 121 is a crucial residue for the specific cytotoxic activity of the ribotoxin alpha-sarcin.2NH and 3OH are crucial structural requirements in sphingomyelin for sticholysin II binding and pore formation in bilayer membranes.Collagen metabolism in human colon adenocarcinoma.Backbone dynamics of the cytotoxic ribonuclease alpha-sarcin by 15N NMR relaxation methods.Tautomeric state of alpha-sarcin histidines. Ndelta tautomers are a common feature in the active site of extracellular microbial ribonucleases.Dissecting structural and electrostatic interactions of charged groups in alpha-sarcin. An NMR study of some mutants involving the catalytic residues.The behavior of sea anemone actinoporins at the water-membrane interface.Conserved asparagine residue 54 of alpha-sarcin plays a role in protein stability and enzyme activity.Role of the basic character of alpha-sarcin's NH2-terminal beta-hairpin in ribosome recognition and phospholipid interaction.Refined NMR structure of alpha-sarcin by 15N-1H residual dipolar couplings.Anomalous electrophoretic behavior of a very acidic protein: ribonuclease U2.Acid phospholipid vesicles produce conformational changes on the antitumour protein alpha-sarcin.Detergent-resistant membranes are platforms for actinoporin pore-forming activity on intact cells.
P50
Q27642493-3C15A835-E867-492E-8863-1FC45C85296EQ27656737-70ECD885-CC53-4DBA-ABC8-029D176697E9Q27667196-88F53B11-07E4-4402-BAF5-891B98271762Q27730427-9D93E370-F02B-4808-A051-932FAF6DAB4BQ30388325-A26B5FC8-9998-46C3-A399-455E87E2834AQ30416920-FBF8A69D-BB2B-4512-8E18-CAC78FD8B4ACQ33419627-3B3A0181-6937-4406-80E5-0864FD980A52Q33959452-80E43568-C616-4B31-B687-DDA7827D1762Q34463322-1F7A3E0A-B355-472E-BB4D-19C589E460CBQ34572784-CEDC59AF-FBE4-436E-A107-0C6A251FD69AQ38196159-1391F69A-6E00-4B00-9C36-DDF425EF5255Q38272432-EAA4F0CA-0BED-4BED-9550-234D23A7A482Q38301637-DA3F43A9-F84D-45CB-9453-1C1D967C3033Q38932236-AF9B6CCA-0B68-4643-95AC-898BE3BBD60AQ39342433-2B70D8B5-16C3-48F9-961A-4A02E400ABB3Q39516903-8882E14D-7CA6-4709-A83E-49C76E70668BQ40243496-05363477-3683-4A55-8AF2-EBFC57C21326Q40424559-AED2EB22-ACA7-4C68-BB45-FCFA9ACE10B5Q41245109-DED666E7-76D2-480F-943A-6F7DBB96CD5BQ41757596-5C5B5813-7EA7-431B-B00A-4F3B1CAF6E83Q41908955-8F74F46F-EF32-464F-BC90-0DB0058A4D59Q42010894-38D83964-5586-4143-8095-E1772834C4CBQ42045164-84461FAC-FB89-4246-8256-5424904DEFF6Q43095229-539C8246-E493-4415-9DAC-147B0A804FEEQ43246626-071EBFE3-42B6-498C-B6BF-87A9ECBF16E7Q43443485-1BF76AEC-83AB-416A-B74F-CF000B9D9417Q43558918-8F209708-6AAB-4FE2-B90F-E94FB32B17BAQ43815486-195AF69D-0C48-4054-871B-46CE08AF616EQ43830914-D78C8B39-BD31-4867-BCEE-BFE241207AF2Q44085204-B13531AF-8229-4B84-9DFF-DE6298AEEC3EQ44274713-BF0BE643-C513-448E-87C6-D1837ABFDE67Q44277961-165ED98A-56D3-4505-9B90-EB4F1D04E6DEQ44649910-2AD33949-BC44-48F0-8039-36966D34AE3FQ44969223-C6424F2E-7D72-424F-BCE5-D7251A929238Q45226426-571D559A-71BD-46CD-B177-A15212604E9AQ46295905-09F6DD2C-E828-4B9F-85BD-DF7E7F0F6B6CQ46424053-3A08CDD4-3198-4103-8EB0-903165387AA0Q46658841-B448BE8A-0E8E-4B1A-AF8F-37D0473CE193Q46894597-428AE766-7F49-4EC7-89BB-F444C8F1C56FQ46915271-9595E7E0-4164-471A-A248-60B0D57FAF80
P50
description
hulumtues
@sq
researcher
@en
wetenschapper
@nl
հետազոտող
@hy
name
José G Gavilanes
@ast
José G Gavilanes
@en
José G Gavilanes
@es
José G Gavilanes
@nl
José G Gavilanes
@sl
type
label
José G Gavilanes
@ast
José G Gavilanes
@en
José G Gavilanes
@es
José G Gavilanes
@nl
José G Gavilanes
@sl
prefLabel
José G Gavilanes
@ast
José G Gavilanes
@en
José G Gavilanes
@es
José G Gavilanes
@nl
José G Gavilanes
@sl
P1053
K-5307-2014
P106
P21
P31
P3829
P496
0000-0002-6852-341X