Promoter methylation and transgene copy numbers predict unstable protein production in recombinant Chinese hamster ovary cell lines.
about
A review of metabolic and enzymatic engineering strategies for designing and optimizing performance of microbial cell factoriesMechanisms underlying epigenetic and transcriptional heterogeneity in Chinese hamster ovary (CHO) cell linesUsing molecular markers to characterize productivity in Chinese hamster ovary cell lines.Evaluating the use of a CpG free promoter for long-term recombinant protein expression stability in Chinese hamster ovary cells.Evaluating the expression profile and stability of different UCOE containing vector combinations in mAb-producing CHO cellsComprehensive genome and epigenome characterization of CHO cells in response to evolutionary pressures and over time.CHO microRNA engineering is growing up: recent successes and future challenges.Establishment of a CpG island microarray for analyses of genome-wide DNA methylation in Chinese hamster ovary cells.Matrix attachment region combinations increase transgene expression in transfected Chinese hamster ovary cellsIdentifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells.The EF-1α promoter maintains high-level transgene expression from episomal vectors in transfected CHO-K1 cells.Genetic analysis of the clonal stability of Chinese hamster ovary cells for recombinant protein production.Cell line development for biomanufacturing processes: recent advances and an outlook.Improved CHO Cell Line Stability and Recombinant Protein Expression During Long-Term Culture.Genetic analyses of Per.C6 cell clones producing a therapeutic monoclonal antibody regarding productivity and long-term stability.High levels of histone H3 acetylation at the CMV promoter are predictive of stable expression in Chinese hamster ovary cells.Deletion of a telomeric region on chromosome 8 correlates with higher productivity and stability of CHO cell lines.Assessment of UCOE on Recombinant EPO Production and Expression Stability in Amplified Chinese Hamster Ovary Cells.Evaluating the interaction between UCOE and DHFR-linked amplification and stability of recombinant protein expression.It's time to regulate: coping with product-induced nongenetic clonal instability in CHO cell lines via regulated protein expression.Fast identification of reliable hosts for targeted cell line development from a limited-genome screening using combined φC31 integrase and CRE-Lox technologies.Determination of Chinese hamster ovary cell line stability and recombinant antibody expression during long-term culture.Chromatin insulator elements block transgene silencing in engineered human embryonic stem cell lines at a defined chromosome 13 locus.CMV promoter mutants with a reduced propensity to productivity loss in CHO cells.Toward stable gene expression in CHO cells.Impact of using different promoters and matrix attachment regions on recombinant protein expression level and stability in stably transfected CHO cells.Insertion of core CpG island element into human CMV promoter for enhancing recombinant protein expression stability in CHO cells.Evaluating the efficiency of CHEF and CMV promoter with IRES and Furin/2A linker sequences for monoclonal antibody expression in CHO cells.First CpG island microarray for genome-wide analyses of DNA methylation in Chinese hamster ovary cells: new insights into the epigenetic answer to butyrate treatment.Engineered and Natural Promoters and Chromatin-Modifying Elements for Recombinant Protein Expression in CHO Cells.CHO-Omics Review: The Impact of Current and Emerging Technologies on Chinese Hamster Ovary Based Bioproduction.Correlation Between Expression of Recombinant Proteins and Abundance of H3K4Me3 on the Enhancer of Human Cytomegalovirus Major Immediate-Early Promoter.Optimized Selection Marker and CHO Host Cell Combinations for Generating High Monoclonal Antibody Producing Cell Lines.Development of a pre-glycoengineered CHO-K1 host cell line for the expression of antibodies with enhanced Fc mediated effector function.Strategies and Considerations for Improving Expression of "Difficult to Express" Proteins in CHO Cells.Whole genome sequencing improves estimation of nuclear DNA content of Chinese hamster ovary cells.
P2860
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P2860
Promoter methylation and transgene copy numbers predict unstable protein production in recombinant Chinese hamster ovary cell lines.
description
2011 nî lūn-bûn
@nan
2011年の論文
@ja
2011年論文
@yue
2011年論文
@zh-hant
2011年論文
@zh-hk
2011年論文
@zh-mo
2011年論文
@zh-tw
2011年论文
@wuu
2011年论文
@zh
2011年论文
@zh-cn
name
Promoter methylation and trans ...... nese hamster ovary cell lines.
@en
Promoter methylation and trans ...... nese hamster ovary cell lines.
@nl
type
label
Promoter methylation and trans ...... nese hamster ovary cell lines.
@en
Promoter methylation and trans ...... nese hamster ovary cell lines.
@nl
prefLabel
Promoter methylation and trans ...... nese hamster ovary cell lines.
@en
Promoter methylation and trans ...... nese hamster ovary cell lines.
@nl
P2093
P2860
P356
P1476
Promoter methylation and trans ...... nese hamster ovary cell lines.
@en
P2093
Andrea Osterlehner
Silke Simmeth
Ulrich Göpfert
P2860
P304
P356
10.1002/BIT.23216
P577
2011-06-15T00:00:00Z