Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris.
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The bud tip is the cellular hot spot of protein secretion in yeasts.Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-carePichia pastoris regulates its gene-specific response to different carbon sources at the transcriptional, rather than the translational, level.Systems-level organization of yeast methylotrophic lifestyle.Enhanced secretion of a methyl parathion hydrolase in Pichia pastoris using a combinational strategy.Comparative genomics and transcriptomics of Pichia pastoris.Dynamic genome-scale metabolic modeling of the yeast Pichia pastorisPichia pastoris Exhibits High Viability and a Low Maintenance Energy Requirement at Near-Zero Specific Growth Rates.Methanol-Independent Protein Expression by AOX1 Promoter with trans-Acting Elements Engineering and Glucose-Glycerol-Shift Induction in Pichia pastoris.Carbon source dependent promoters in yeasts.Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production.Metabolic modeling to identify engineering targets for Komagataella phaffii: The effect of biomass composition on gene target identification.Recent advances in the production of recombinant subunit vaccines in Pichia pastoris.Overexpression of the transcription factor Yap1 modifies intracellular redox conditions and enhances recombinant protein secretion.Secreted expression of truncated capsid protein from porcine circovirus type 2 in Pichia pastoris.Overexpressing target helper genes enhances secretion and glycosylation of recombinant proteins in Pichia pastoris under simulated microgravity.Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector optimization.Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology.A novel bi-directional promoter system allows tunable recombinant protein production in Pichia pastorisA Novel Vector for Construction of Markerless Multicopy Overexpression Transformants in Pichia pastoris.In Pichia pastoris, growth rate regulates protein synthesis and secretion, mating and stress response.Non-canonical integration events in Pichia pastoris encountered during standard transformation analysed with genome sequencing.Effects of glycerol supply and specific growth rate on methanol-free production of CALB by P. pastoris: functional characterisation of a novel promoter.Pichia pastoris secretes recombinant proteins less efficiently than Chinese hamster ovary cells but allows higher space-time yields for less complex proteinsPlaying catch-up with Escherichia coli: using yeast to increase success rates in recombinant protein production experiments.A Mitochondrial Autonomously Replicating Sequence from Pichia pastoris for Uniform High Level Recombinant Protein Production.Pichia pastoris Mut(S) strains are prone to misincorporation of O-methyl-L-homoserine at methionine residues when methanol is used as the sole carbon source.Fine-tuning the P. pastoris iMT1026 genome-scale metabolic model for improved prediction of growth on methanol or glycerol as sole carbon sources.GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris.Systems biotechnology for protein production in Pichia pastoris.CamOptimus: a tool for exploiting complex adaptive evolution to optimize experiments and processes in biotechnology.The vitamin-sensitive promoter PTHI11 enables pre-defined autonomous induction of recombinant protein production in Pichia pastoris.Molecular strategies to increase the levels of heterologous transcripts in Komagataella phaffii for protein production.
P2860
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P2860
Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris.
description
2013 nî lūn-bûn
@nan
2013年の論文
@ja
2013年論文
@yue
2013年論文
@zh-hant
2013年論文
@zh-hk
2013年論文
@zh-mo
2013年論文
@zh-tw
2013年论文
@wuu
2013年论文
@zh
2013年论文
@zh-cn
name
Induction without methanol: no ...... expression in Pichia pastoris.
@en
Induction without methanol: no ...... expression in Pichia pastoris.
@nl
type
label
Induction without methanol: no ...... expression in Pichia pastoris.
@en
Induction without methanol: no ...... expression in Pichia pastoris.
@nl
prefLabel
Induction without methanol: no ...... expression in Pichia pastoris.
@en
Induction without methanol: no ...... expression in Pichia pastoris.
@nl
P2093
P2860
P50
P356
P1476
Induction without methanol: no ...... expression in Pichia pastoris
@en
P2093
Christoph Kiziak
Jana Wenger
Joachim Klein
Michael Maurer
P2860
P2888
P356
10.1186/1475-2859-12-5
P577
2013-01-24T00:00:00Z
P5875
P6179
1049713105