A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures
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The fed-batch principle for the molecular biology lab: controlled nutrient diets in ready-made media improve production of recombinant proteins in Escherichia coliPeroxisomal multifunctional enzyme type 2 from the fruitfly: dehydrogenase and hydratase act as separate entities, as revealed by structure and kineticsHigh Yield of Recombinant Protein in Shaken E. coli Cultures with Enzymatic Glucose Release Medium EnPresso B.Novel approach of high cell density recombinant bioprocess development: optimisation and scale-up from microliter to pilot scales while maintaining the fed-batch cultivation mode of E. coli cultures.Production of recombinant human annexin V by fed-batch cultivation.Controlling pH in shake flasks using polymer-based controlled-release discs with pre-determined release kinetics.Reducing conditions are the key for efficient production of active ribonuclease inhibitor in Escherichia coli.Parallel production and verification of protein products using a novel high-throughput screening method.Production of gamma-aminobutyric acid by Lactobacillus brevis NCL912 using fed-batch fermentationExpression of the functional recombinant human glycosyltransferase GalNAcT2 in Escherichia coliProtective activity of the CnaBE3 domain conserved among Staphylococcus aureus Sdr proteins.Fed-batch like cultivation in a micro-bioreactor: screening conditions relevant for Escherichia coli based production processes.The influence of microbial physiology on biocatalyst activity and efficiency in the terminal hydroxylation of n-octane using Escherichia coli expressing the alkane hydroxylase, CYP153A6.Expression of Codon-Optimized Plant Glycosyltransferase UGT72B14 in Escherichia coli Enhances Salidroside Production.High-yield production of functional soluble single-domain antibodies in the cytoplasm of Escherichia coli.Enzyme-based glucose delivery: a possible tool for biosorbent preparation for heavy metal removal from polluted environments.Development of a cultivation process for the enhancement of human interferon alpha 2b production in the oleaginous yeast, Yarrowia lipolytica.High-yield production of biologically active recombinant protein in shake flask culture by combination of enzyme-based glucose delivery and increased oxygen transfer.Systematic evaluation of characteristics of the membrane-based fed-batch shake flask.High cell density cultivation and recombinant protein production with Escherichia coli in a rocking-motion-type bioreactorThe Staphylococcus aureus NuoL-like protein MpsA contributes to the generation of membrane potential.Parallel use of shake flask and microtiter plate online measuring devices (RAMOS and BioLector) reduces the number of experiments in laboratory-scale stirred tank bioreactors.Effect of culture medium, host strain and oxygen transfer on recombinant Fab antibody fragment yield and leakage to medium in shaken E. coli cultures.High-level fed-batch fermentative expression of an engineered Staphylococcal protein A based ligand in E. coli: purification and characterizationMetabolic engineering of Bacillus subtilis for growth on overflow metabolites.An Air-Well sparging minifermenter system for high-throughput protein productionIncreased bacterial cell density and recombinant protein yield using a commercial microbial cultivation system.Acetate: friend or foe? Efficient production of a sweet protein in Escherichia coli BL21 using acetate as a carbon source.Scale-up bioprocess development for production of the antibiotic valinomycin in Escherichia coli based on consistent fed-batch cultivations.Sandwich ELISA for quantitative detection of human collagen prolyl 4-hydroxylase.Enhanced growth and recombinant protein production of Escherichia coli by a perfluorinated oxygen carrier in miniaturized fed-batch cultures.Enhanced plasmid production in miniaturized high-cell-density cultures of Escherichia coli supported with perfluorinated oxygen carrier.Direct and indirect use of GFP whole cell biosensors for the assessment of bioprocess performances: design of milliliter scale-down bioreactors.Small-scale slow glucose feed cultivation of Pichia pastoris without repression of AOX1 promoter: towards high throughput cultivations.Efficient Antibody Assembly in E. coli Periplasm by Disulfide Bond Folding Factor Co-expression and Culture Optimization.A novel fed-batch based strategy for enhancing cell-density and recombinant cyprosin B production in bioreactors.Application of termite hindgut metagenome derived carboxyl ester hydrolases in the modification of cephalosporin substrates.Feeding strategies enhance high cell density cultivation and protein expression in milliliter scale bioreactors.Cytosolic expression of functional Fab fragments in Escherichia coli using a novel combination of dual SUMO expression cassette and EnBase® cultivation mode.Enhanced production of the nonribosomal peptide antibiotic valinomycin in Escherichia coli through small-scale high cell density fed-batch cultivation.
P2860
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P2860
A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures
description
2010 nî lūn-bûn
@nan
2010年の論文
@ja
2010年学术文章
@wuu
2010年学术文章
@zh-cn
2010年学术文章
@zh-hans
2010年学术文章
@zh-my
2010年学术文章
@zh-sg
2010年學術文章
@yue
2010年學術文章
@zh
2010年學術文章
@zh-hant
name
A novel fed-batch based cultiv ...... nt proteins in shaken cultures
@en
A novel fed-batch based cultiv ...... t proteins in shaken cultures.
@nl
type
label
A novel fed-batch based cultiv ...... nt proteins in shaken cultures
@en
A novel fed-batch based cultiv ...... t proteins in shaken cultures.
@nl
prefLabel
A novel fed-batch based cultiv ...... nt proteins in shaken cultures
@en
A novel fed-batch based cultiv ...... t proteins in shaken cultures.
@nl
P2093
P2860
P356
P1476
A novel fed-batch based cultiv ...... nt proteins in shaken cultures
@en
P2093
Antje Neubauer
Antti Vasala
Kaisa Ukkonen
Maria Ruottinen
Mirja Krause
Peter Neubauer
Tatu Haataja
Tuomo Glumoff
P2860
P2888
P356
10.1186/1475-2859-9-11
P577
2010-02-19T00:00:00Z
P5875
P6179
1004016470