A possible aid in targeted insertion of large DNA elements by CRISPR/Cas in mouse zygotes.
about
Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins.Culture time of vitrified/warmed zygotes before microinjection affects the production efficiency of CRISPR-Cas9-mediated knock-in mice.Next-generation mammalian genetics toward organism-level systems biologyCRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome.Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice.CRISPR/Cas9-mediated gene manipulation to create single-amino-acid-substituted and floxed mice with a cloning-free method.Optimization of the production of knock-in alleles by CRISPR/Cas9 microinjection into the mouse zygote.Efficient generation of mice carrying homozygous double-floxp alleles using the Cas9-Avidin/Biotin-donor DNA system.Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors.Production of knock-in mice in a single generation from embryonic stem cells.Noninheritable Maternal Factors Useful for Genetic Manipulation in Mammals.
P2860
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P2860
A possible aid in targeted insertion of large DNA elements by CRISPR/Cas in mouse zygotes.
description
2015 nî lūn-bûn
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name
A possible aid in targeted ins ...... y CRISPR/Cas in mouse zygotes.
@en
type
label
A possible aid in targeted ins ...... y CRISPR/Cas in mouse zygotes.
@en
prefLabel
A possible aid in targeted ins ...... y CRISPR/Cas in mouse zygotes.
@en
P2093
P2860
P356
P1433
P1476
A possible aid in targeted ins ...... y CRISPR/Cas in mouse zygotes.
@en
P2093
Harumi Nakao
Hiroshi Kiyonari
Kazuki Nakao
Kenichi Inoue
Takeshi Harada
Yasuhide Furuta
P2860
P356
10.1002/DVG.22914
P50
P577
2015-12-29T00:00:00Z