Elimination of contaminating DNA within polymerase chain reaction reagents: implications for a general approach to detection of uncultured pathogens.
about
Contamination and sensitivity issues with a real-time universal 16S rRNA PCRInhibition and facilitation of nucleic acid amplificationAn efficient multistrategy DNA decontamination procedure of PCR reagents for hypersensitive PCR applicationsOptimizing Taq polymerase concentration for improved signal-to-noise in the broad range detection of low abundance bacteriaDetection of prosthetic hip infection at revision arthroplasty by immunofluorescence microscopy and PCR amplification of the bacterial 16S rRNA gene.Helicobacter pylori: clonal population structure and restricted transmission within families revealed by molecular typing.BactQuant: an enhanced broad-coverage bacterial quantitative real-time PCR assay.Risk assessment models and contamination management: implications for broad-range ribosomal DNA PCR as a diagnostic tool in medical bacteriologyRapid 16S rRNA next-generation sequencing of polymicrobial clinical samples for diagnosis of complex bacterial infections.Evaluation of an ethidium monoazide-enhanced 16S rDNA real-time polymerase chain reaction assay for bacterial screening of platelet concentrates and comparison with automated cultureRapid detection of hepatitis B virus in blood plasma by a specific and sensitive loop-mediated isothermal amplification assayPreparation of Phi29 DNA polymerase free of amplifiable DNA using ethidium monoazide, an ultraviolet-free light-emitting diode lamp and trehalose.Molecular diagnosis of culture negative infective endocarditis: clinical validation in a group of surgically treated patients.Eukaryote-Made Thermostable DNA Polymerase Enables Rapid PCR-Based Detection of Mycoplasma, Ureaplasma and Other Bacteria in the Amniotic Fluid of Preterm Labor Cases.Removal of Contaminant DNA by Combined UV-EMA Treatment Allows Low Copy Number Detection of Clinically Relevant Bacteria Using Pan-Bacterial Real-Time PCRIsolation and characterization of a new strain of Ehrlichia chaffeensis from a patient with nearly fatal monocytic ehrlichiosisMolecular identification of bacteria by fluorescence-based PCR-single-strand conformation polymorphism analysis of the 16S rRNA geneFactors affecting reliability and reproducibility of amplification-based DNA fingerprinting of representative bacterial pathogens.Characterization of an immunoreactive protein from the agent of human granulocytic ehrlichiosis.Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human disease.Development of PCR assays to detect ampicillin resistance genes in cerebrospinal fluid samples containing Haemophilus influenzae.Rapid identification of bacteria by PCR-single-strand conformation polymorphismPreventing false positives: quantitative evaluation of three protocols for inactivation of polymerase chain reaction amplification productsAn assessment of air as a source of DNA contamination encountered when performing PCRTwo novel real-time reverse transcriptase PCR assays for rapid detection of bacterial contamination in platelet concentrates.A novel eukaryote-made thermostable DNA polymerase which is free from bacterial DNA contamination.Emperor's new clothes: Is particle disease really infected particle disease?Gram type-specific broad-range PCR amplification for rapid detection of 62 pathogenic bacteria.Elimination of bacterial DNA from Taq DNA polymerases by restriction endonuclease digestion.Direct detection of eae-positive bacteria in human and veterinary colorectal specimens by PCR16S ribosomal DNA-based analysis of bacterial diversity in purified water used in pharmaceutical manufacturing processes by PCR and denaturing gradient gel electrophoresis.DNase pretreatment of master mix reagents improves the validity of universal 16S rRNA gene PCR results.Multiplex PCR using conserved and species-specific 16S rRNA gene primers for simultaneous detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalisMolecular diagnosis of cat scratch disease: a two-step approach.Identification and elimination of DNA sequences in Taq DNA polymerase.Identification of mutations in 23S rRNA gene of clarithromycin-resistant Mycobacterium intracellulare.Presence of bacterial phage-like DNA sequences in commercial Taq DNA polymerase reagentsFour-year bacterial monitoring in the International Space Station-Japanese Experiment Module "Kibo" with culture-independent approach.
P2860
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P2860
Elimination of contaminating DNA within polymerase chain reaction reagents: implications for a general approach to detection of uncultured pathogens.
description
1993 nî lūn-bûn
@nan
1993年の論文
@ja
1993年論文
@yue
1993年論文
@zh-hant
1993年論文
@zh-hk
1993年論文
@zh-mo
1993年論文
@zh-tw
1993年论文
@wuu
1993年论文
@zh
1993年论文
@zh-cn
name
Elimination of contaminating D ...... ction of uncultured pathogens.
@en
type
label
Elimination of contaminating D ...... ction of uncultured pathogens.
@en
prefLabel
Elimination of contaminating D ...... ction of uncultured pathogens.
@en
P2093
P2860
P1476
Elimination of contaminating D ...... ection of uncultured pathogens
@en
P2093
P2860
P304
P407
P577
1993-03-01T00:00:00Z