Demonstration of preferential binding of SYBR Green I to specific DNA fragments in real-time multiplex PCR.
about
Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implicationsSensitive Melting Analysis after Real Time- Methylation Specific PCR (SMART-MSP): high-throughput and probe-free quantitative DNA methylation detectionSimultaneous detection of Fusarium culmorum and F. graminearum in plant material by duplex PCR with melting curve analysis.Multiplex qPCR for serodetection and serotyping of hepatitis viruses: A brief reviewMethods for molecular surveillance of influenza.A matrix gene-based multiplex real-time RT-PCR for detection and differentiation of 2009 pandemic H1N1 and other influenza A viruses in North America.Updated values for molecular diagnosis for highly pathogenic avian influenza virus.Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications.High resolution melting analysis for the rapid and sensitive detection of mutations in clinical samples: KRAS codon 12 and 13 mutations in non-small cell lung cancer.Use of DNA melting simulation software for in silico diagnostic assay design: targeting regions with complex melting curves and confirmation by real-time PCR using intercalating dyes.High-throughput avian molecular sexing by SYBR green-based real-time PCR combined with melting curve analysis.A comprehensive collection of experimentally validated primers for Polymerase Chain Reaction quantitation of murine transcript abundance.Fluorescent duplex allele-specific PCR and amplicon melting for rapid homogeneous mtDNA haplogroup H screening and sensitive mixture detection.SYTO dyes and EvaGreen outperform SYBR Green in real-time PCRA one-step real-time multiplex PCR for screening Y-chromosomal microdeletions without downstream amplicon size analysis.Detection of alpha-thalassemia-1 Southeast Asian type using real-time gap-PCR with SYBR Green1 and high resolution melting analysis.Sex determination in highly fragmented human DNA by high-resolution melting (HRM) analysisInteraction of pyrrolobenzodiazepine (PBD) ligands with parallel intermolecular G-quadruplex complex using spectroscopy and ESI-MS.Validation of the β-amy1 transcription profiling assay and selection of reference genes suited for a RT-qPCR assay in developing barley caryopsis.Development and application of a universal Hemoplasma screening assay based on the SYBR green PCR principleAmplicon DNA melting analysis for the simultaneous detection of Brucella spp and Mycobacterium tuberculosis complex. Potential use in rapid differential diagnosis between extrapulmonary tuberculosis and focal complications of brucellosisDICER1 expression and outcomes in endometrioid endometrial adenocarcinoma.Charging YOYO-1 on capillary wall for online DNA intercalation and integrating this approach with multiplex PCR and bare narrow capillary-hydrodynamic chromatography for online DNA analysisDetection of α-thalassemia-1 Southeast Asian and Thai type deletions and β-thalassemia 3.5-kb deletion by single-tube multiplex real-time PCR with SYBR Green1 and high-resolution melting analysis.Assessment of high resolution melting analysis as a potential SNP genotyping technique in forensic casework.Identification of the GST-T1 and GST-M1 null genotypes using high resolution melting analysisIdentification of Suitable Reference Genes for Gene Expression Normalization in the Quantitative Real-Time PCR Analysis of Sweet Osmanthus (Osmanthus fragrans Lour.).High-resolution DNA melt curve analysis of the clustered, regularly interspaced short-palindromic-repeat locus of Campylobacter jejuni.A reliable method for quantification of splice variants using RT-qPCR.MeltMan: Optimization, Evaluation, and Universal Application of a qPCR System Integrating the TaqMan qPCR and Melting Analysis into a Single Assay.A novel gap-PCR with high resolution melting analysis for the detection of α-thalassaemia Southeast Asian and Filipino β°-thalassaemia deletion.Comparison of multiple DNA dyes for real-time PCR: effects of dye concentration and sequence composition on DNA amplification and melting temperature.Simultaneous detection of Listeria monocytogenes and Salmonella spp. in dairy products using real time PCR-melt curve analysis.A new approach to varietal identification in plants by microsatellite high resolution melting analysis: application to the verification of grapevine and olive cultivars.Primase-based whole genome amplification.A novel label-free fluorescence assay for one-step sensitive detection of Hg2+ in environmental drinking water samplesUse of qPCR for the study of hepatotoxic cyanobacteria population dynamics.Improving the understanding of oligonucleotide-nanoparticle conjugates using DNA-binding fluorophores.Development of a single-tube multiplex real-time PCR for detection and identification of five pathogenic targets by using melting-curve analysis with EvaGreen.Experimental analysis of gene assembly with TopDown one-step real-time gene synthesis.
P2860
Q24563152-6147E886-9566-43E3-A355-20DA848D3241Q24646396-DFDB3BDC-CF5B-48F4-9D7A-2451D879B3ADQ25257795-D7EC83B4-1465-43B1-B20C-959A3AC3602FQ26745451-7DDC652C-2842-423E-8F34-725806CA4EACQ30388975-841CA365-40AB-4172-B45B-3D4B1D655F25Q30395046-27D1F269-A8A8-45E2-B578-F4CC1BBD7DF0Q30421636-91B31ECD-2634-4A87-B9B7-F61B4EBACFDFQ30837445-982F7860-9E3F-4479-A13B-E827E81E72F6Q33267389-EB982B00-BA80-4C94-A5EB-DE9F319F093AQ33280321-456EC6FB-C086-4A4E-A7D7-A9BF5AA232FCQ33319520-853691E4-B822-42DB-AF27-FF10232F811CQ33395881-52A58C94-4C7D-495D-8ACB-608BC0CB99AFQ33519569-7D5CF200-D48A-4B6F-897A-CAACB98E3D12Q33974242-25005006-E049-4DF3-AD21-6D37FA3B69AFQ34009289-44D67F1B-5560-4C78-96A1-63821342C3FDQ34010000-BBE32F53-9125-40C4-A184-0F83A42FAEF2Q34011312-BFB85589-4C59-40FC-A1FE-46E7789A6F91Q34256936-A9CA379D-13EE-4699-8B09-338E873AF1D2Q34364270-9C78C64F-18BC-40E7-88B6-CEFE28AF547AQ34612790-B24BD18D-FD67-4D18-9D4D-F8FE173B820CQ34631778-E596FF83-D8F0-43A7-BDB0-946F687C76F4Q34731304-2D19F57E-787D-454B-9712-6200A5AD5133Q35054091-D878D2E7-D28F-453A-8EF1-714F39D0E5A0Q35085497-C58F7B8C-E32F-4443-A006-25BA47688601Q35228903-B17070C7-F2B8-40C4-9FBA-8B829BD185A0Q35684516-23D2C6EC-B59D-48F9-9F5B-38135E0E661AQ35753800-B5B5670A-26FF-4F6E-B429-845D72642846Q35869736-767C63DC-E3D1-4D35-B272-76DE05108BE0Q35958211-72F70851-86D7-4B03-B25D-1C91C0C6289EQ35975451-88625E6E-5F52-4D04-A06B-AF90D6DF08F0Q36056274-140177C1-68A7-4FB1-B033-A20C0147C7ABQ36173027-9652D3D8-0251-4515-AE62-837C977019B8Q36550282-C2B8EF12-1388-4849-B3ED-8B9E02EE2642Q36677030-E5BCCBBB-B9C3-4860-81D3-09D4830F7BDAQ36798701-C9F77ABB-0856-4FBE-9B34-C43E113DE8B0Q37737886-FEB0F88B-01AA-4C31-9941-6D9A0D4548DBQ37898572-33A8BB7C-EF3F-4648-B788-FB886217D06FQ38316202-67E83E84-9812-4BAA-A874-FF13DDD3893DQ38320904-336361A4-E799-4835-A46C-A2DCA31ECF4DQ38356158-0B2315E2-F971-437E-A52E-CD3B121ED182
P2860
Demonstration of preferential binding of SYBR Green I to specific DNA fragments in real-time multiplex PCR.
description
2003 nî lūn-bûn
@nan
2003年の論文
@ja
2003年論文
@yue
2003年論文
@zh-hant
2003年論文
@zh-hk
2003年論文
@zh-mo
2003年論文
@zh-tw
2003年论文
@wuu
2003年论文
@zh
2003年论文
@zh-cn
name
Demonstration of preferential ...... ts in real-time multiplex PCR.
@en
type
label
Demonstration of preferential ...... ts in real-time multiplex PCR.
@en
prefLabel
Demonstration of preferential ...... ts in real-time multiplex PCR.
@en
P2093
P2860
P356
P1476
Demonstration of preferential ...... ts in real-time multiplex PCR.
@en
P2093
Christopher P Saint
Paul T Monis
Steven Giglio
P2860
P356
10.1093/NAR/GNG135
P577
2003-11-01T00:00:00Z