Roles of a 106-bp origin enhancer and Escherichia coli DnaA protein in replication of plasmid R6K.
about
Transposon assisted gene insertion technology (TAGIT): a tool for generating fluorescent fusion proteins.Dimers of pi protein bind the A+T-rich region of the R6K gamma origin near the leading-strand synthesis start sites: regulatory implicationsAT-rich region and repeated sequences - the essential elements of replication origins of bacterial replicons.A DNA segment conferring stable maintenance on R6K gamma-origin core replicons.Novel alleles of the Escherichia coli dnaA gene are defective in replication of pSC101 but not of oriC.Preponderance of Fis-binding sites in the R6K gamma origin and the curious effect of the penicillin resistance marker on replication of this origin in the absence of FisBinding of DnaA protein to a replication enhancer counteracts the inhibition of plasmid R6K gamma origin replication mediated by elevated levels of R6K pi proteinIdentification of a novel promoter in the replication control region of plasmid R6KPlasmid R6K replication controlReconstitution of R6K DNA replication in vitro using 22 purified proteins.Boundaries of the pSC101 minimal replicon are conditional.Altered (copy-up) forms of initiator protein pi suppress the point mutations inactivating the gamma origin of plasmid R6K.Cooperative binding of initiator protein to replication origin conferred by single amino acid substitution.A critical DnaA box directs the cooperative binding of the Escherichia coli DnaA protein to the plasmid RK2 replication origin.Role of TrfA and DnaA proteins in origin opening during initiation of DNA replication of the broad host range plasmid RK2.Insertional transposon mutagenesis by electroporation of released Tn5 transposition complexes.
P2860
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P2860
Roles of a 106-bp origin enhancer and Escherichia coli DnaA protein in replication of plasmid R6K.
description
1992 nî lūn-bûn
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1992年の論文
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1992年学术文章
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1992年学术文章
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1992年学术文章
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name
Roles of a 106-bp origin enhan ...... in replication of plasmid R6K.
@en
type
label
Roles of a 106-bp origin enhan ...... in replication of plasmid R6K.
@en
prefLabel
Roles of a 106-bp origin enhan ...... in replication of plasmid R6K.
@en
P2093
P2860
P356
P1476
Roles of a 106-bp origin enhan ...... in replication of plasmid R6K.
@en
P2093
P2860
P304
P356
10.1093/NAR/20.4.811
P577
1992-02-01T00:00:00Z