Codon optimization of Caenorhabditis elegans GluCl ion channel genes for mammalian cells dramatically improves expression levels.
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Autonomous bioluminescent expression of the bacterial luciferase gene cassette (lux) in a mammalian cell lineMultiple-color optical activation, silencing, and desynchronization of neural activity, with single-spike temporal resolutionExpression of human nPTB is limited by extreme suboptimal codon contentPrinciples of activation and permeation in an anion-selective Cys-loop receptorChanging channels in pain and epilepsy: Exploiting ion channel gene therapy for disorders of neuronal hyperexcitabilityTwo rare variations, D478N and D478E, that occur at the same amino acid residue in nicotinic acetylcholine receptor (nAChR) α2 subunit influence nAChR function.A signal peptide missense mutation associated with nicotine dependence alters α2*-nicotinic acetylcholine receptor function.Analysis of rare variations reveals roles of amino acid residues in the N-terminal extracellular domain of nicotinic acetylcholine receptor (nAChR) alpha6 subunit in the functional expression of human alpha6*-nAChRs.Nicotinic acetylcholine receptor alpha7 subunits with a C2 cytoplasmic loop yellow fluorescent protein insertion form functional receptors.Genetic dissection of an amygdala microcircuit that gates conditioned fearAnalysis of the distribution of functionally relevant rare codons.Looking for inspiration: new perspectives on respiratory rhythm.Specificity and efficiency of reporter expression in adult neural progenitors vary substantially among nestin-CreER(T2) lines.Visual gene developer: a fully programmable bioinformatics software for synthetic gene optimization.α7β2 nicotinic acetylcholine receptors assemble, function, and are activated primarily via their α7-α7 interfaces.Functional evaluation of key interactions evident in the structure of the eukaryotic Cys-loop receptor GluCl.Remote control of neuronal signaling.Functional characterization of neurotransmitter activation and modulation in a nematode model ligand-gated ion channel.An engineered glutamate-gated chloride (GluCl) channel for sensitive, consistent neuronal silencing by ivermectin.Single-molecule imaging of a fluorescent unnatural amino acid incorporated into nicotinic receptorsTranslation elongation can control translation initiation on eukaryotic mRNAs.The structural basis of function in Cys-loop receptors.Manipulating the genetic code for membrane protein production: what have we learnt so far?Unparalleled control of neural activity using orthogonal pharmacogenetics.Rapidly inducible, genetically targeted inactivation of neural and synaptic activity in vivoAcute inhibition of a cortical motor area impairs vocal control in singing zebra finches.The antihelminthic moxidectin enhances tonic GABA currents in rodent hippocampal pyramidal neurons.Improved production of membrane proteins in Escherichia coli by selective codon substitutions.Mammalian expression levels of cellulase and xylanase genes optimised by human codon usage are not necessarily higher than those optimised by the extremely biased approach.
P2860
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P2860
Codon optimization of Caenorhabditis elegans GluCl ion channel genes for mammalian cells dramatically improves expression levels.
description
2003 nî lūn-bûn
@nan
2003年の論文
@ja
2003年論文
@yue
2003年論文
@zh-hant
2003年論文
@zh-hk
2003年論文
@zh-mo
2003年論文
@zh-tw
2003年论文
@wuu
2003年论文
@zh
2003年论文
@zh-cn
name
Codon optimization of Caenorha ...... ly improves expression levels.
@en
type
label
Codon optimization of Caenorha ...... ly improves expression levels.
@en
prefLabel
Codon optimization of Caenorha ...... ly improves expression levels.
@en
P1476
Codon optimization of Caenorha ...... lly improves expression levels
@en
P2093
Eric M Slimko
P356
10.1016/S0165-0270(02)00362-X
P50
P577
2003-03-01T00:00:00Z