Generation and iterative affinity maturation of antibodies in vitro using hypermutating B-cell lines.
about
Mutational comparison of the single-domained APOBEC3C and double-domained APOBEC3F/G anti-retroviral cytidine deaminases provides insight into their DNA target site specificitiesGenetic manipulation of an exogenous non-immunoglobulin protein by gene conversion machinery in a chicken B cell lineStable long-term cultures of self-renewing B cells and their applicationsStructural consequences of target epitope-directed functional alteration of an antibody. The case of anti-hen lysozyme antibody, HyHEL-10Molecular evolution of antibody cross-reactivity for two subtypes of type A botulinum neurotoxinAntibody discovery ex vivo accelerated by the LacO/LacI regulatory networkIn vitro antibody evolution targeting germline hot spots to increase activity of an anti-CD22 immunotoxin.Rapid generation of specific antibodies by enhanced homologous recombination.Laboratory-directed protein evolutionEngineering of monoclonal antibodies and antibody-based fusion proteins: successes and challenges.Isolation of anti-CD22 Fv with high affinity by Fv display on human cells.Lentivirus display: stable expression of human antibodies on the surface of human cells and virus particlesDevelopment of a novel mammalian cell surface antibody display platformSpatially addressed combinatorial protein libraries for recombinant antibody discovery and optimization.B-cell display-based one-step method to generate chimeric human IgG monoclonal antibodiesCoupling mammalian cell surface display with somatic hypermutation for the discovery and maturation of human antibodiesAffinity maturation of anti-TNF-alpha scFv with somatic hypermutation in non-B cells.Harnessing gene conversion in chicken B cells to create a human antibody sequence repertoire.Conditional human VEGF-mediated vascularization in chicken embryos using a novel temperature-inducible gene regulation (TIGR) systemRecombinase-mediated cassette exchange as a novel method to study somatic hypermutation in Ramos cellsA Novel Dual Expression Platform for High Throughput Functional Screening of Phage Libraries in Product like FormatDirected evolution of human scFvs in DT40 cells.Protein evolution by hypermutation and selection in the B cell line DT40Modulation of immunoglobulin gene conversion in chicken DT40 by enhancing histone acetylation, and its application to antibody engineering.Activation-induced cytidine deaminase-mediated hypermutation in the DT40 cell line.Reconstituted B cell receptor signaling reveals carbohydrate-dependent mode of activation.In vitro reconstitution of B cell receptor-antigen interactions to evaluate potential vaccine candidates.Directed evolution of mammalian anti-apoptosis proteins by somatic hypermutationAssessing somatic hypermutation in Ramos B cells after overexpression or knockdown of specific genes.Human monoclonal antibodies to HIV-1 gp140 from mice bearing YAC-based human immunoglobulin transloci.AID upmutants isolated using a high-throughput screen highlight the immunity/cancer balance limiting DNA deaminase activity.Humanization of antibodies using heavy chain complementarity-determining region 3 grafting coupled with in vitro somatic hypermutationDirected evolution of an angiopoietin-2 ligand trap by somatic hypermutation and cell surface display.Stochasticity enables BCR-independent germinal center initiation and antibody affinity maturation.Coupling recombinase-mediated cassette exchange with somatic hypermutation for antibody affinity maturation in CHO cells.Transient AID expression for in situ mutagenesis with improved cellular fitness.
P2860
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P2860
Generation and iterative affinity maturation of antibodies in vitro using hypermutating B-cell lines.
description
2002 nî lūn-bûn
@nan
2002年の論文
@ja
2002年論文
@yue
2002年論文
@zh-hant
2002年論文
@zh-hk
2002年論文
@zh-mo
2002年論文
@zh-tw
2002年论文
@wuu
2002年论文
@zh
2002年论文
@zh-cn
name
Generation and iterative affin ...... ng hypermutating B-cell lines.
@en
type
label
Generation and iterative affin ...... ng hypermutating B-cell lines.
@en
prefLabel
Generation and iterative affin ...... ng hypermutating B-cell lines.
@en
P2093
P356
P1433
P1476
Generation and iterative affin ...... ng hypermutating B-cell lines.
@en
P2093
Facundo D Batista
Gareth T Williams
Michael S Neuberger
Richard L Grenfell
Sarah J Cumbers
Sarah L Davies
P2888
P304
P356
10.1038/NBT752
P577
2002-10-15T00:00:00Z