A versatile, efficient strategy for assembly of multi-fragment expression vectors in Saccharomyces cerevisiae using 60 bp synthetic recombination sequences.
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Rational Design of High-Number dsDNA Fragments Based on Thermodynamics for the Construction of Full-Length Genes in a Single ReactionPathway swapping: Toward modular engineering of essential cellular processes.Engineering acetyl coenzyme A supply: functional expression of a bacterial pyruvate dehydrogenase complex in the cytosol of Saccharomyces cerevisiae.Recent advances in DNA assembly technologiesEfficient construct of a large and functional scFv yeast display library derived from the ascites B cells of ovarian cancer patients by three-fragment transformation-associated recombination.Modular pathway rewiring of Saccharomyces cerevisiae enables high-level production of L-ornithine.EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9Advances in metabolic engineering of yeast Saccharomyces cerevisiae for production of chemicals.CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae.Building biological foundries for next-generation synthetic biology.High molecular weight DNA assembly in vivo for synthetic biology applications.Pathway Design, Engineering, and Optimization.One-step assembly and targeted integration of multigene constructs assisted by the I-SceI meganuclease in Saccharomyces cerevisiae.CrEdit: CRISPR mediated multi-loci gene integration in Saccharomyces cerevisiae.Yeast recombination-based cloning as an efficient way of constructing vectors for Zymoseptoria triticiA new laboratory evolution approach to select for constitutive acetic acid tolerance in Saccharomyces cerevisiae and identification of causal mutations.Carbon dioxide fixation by Calvin-Cycle enzymes improves ethanol yield in yeastElimination of sucrose transport and hydrolysis in Saccharomyces cerevisiae: a platform strain for engineering sucrose metabolism.FnCpf1: a novel and efficient genome editing tool for Saccharomyces cerevisiae.CRISPR-Cas9 mediated gene deletions in lager yeast Saccharomyces pastorianus.Excessive by-product formation: A key contributor to low isobutanol yields of engineered Saccharomyces cerevisiae strains.Laboratory Evolution of a Biotin-Requiring Saccharomyces cerevisiae Strain for Full Biotin Prototrophy and Identification of Causal Mutations.Improving heterologous production of phenylpropanoids in Saccharomyces cerevisiae by tackling an unwanted side reaction of Tsc13, an endogenous double-bond reductase.Genome editing in Kluyveromyces and Ogataea yeasts using a broad-host-range Cas9/gRNA co-expression plasmid.Replacement of the initial steps of ethanol metabolism in Saccharomyces cerevisiae by ATP-independent acetylating acetaldehyde dehydrogenase.Rapid and marker-free refactoring of xylose-fermenting yeast strains with Cas9/CRISPR.Low affinity uniporter carrier proteins can increase net substrate uptake rate by reducing efflux.The Penicillium chrysogenum transporter PcAraT enables high-affinity, glucose-insensitive l-arabinose transport in Saccharomyces cerevisiae.Laboratory evolution for forced glucose-xylose co-consumption enables identification of mutations that improve mixed-sugar fermentation by xylose-fermenting Saccharomyces cerevisiae.Engineering de novo anthocyanin production in Saccharomyces cerevisiae.
P2860
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P2860
A versatile, efficient strategy for assembly of multi-fragment expression vectors in Saccharomyces cerevisiae using 60 bp synthetic recombination sequences.
description
2013 nî lūn-bûn
@nan
2013年の論文
@ja
2013年学术文章
@wuu
2013年学术文章
@zh-cn
2013年学术文章
@zh-hans
2013年学术文章
@zh-my
2013年学术文章
@zh-sg
2013年學術文章
@yue
2013年學術文章
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2013年學術文章
@zh-hant
name
A versatile, efficient strateg ...... hetic recombination sequences.
@en
type
label
A versatile, efficient strateg ...... hetic recombination sequences.
@en
prefLabel
A versatile, efficient strateg ...... hetic recombination sequences.
@en
P2093
P2860
P50
P356
P1476
A versatile, efficient strateg ...... thetic recombination sequences
@en
P2093
Daniel Solis-Escalante
Lizanne Bosman
Marcel van den Broek
Niels G A Kuijpers
P2860
P2888
P356
10.1186/1475-2859-12-47
P577
2013-05-10T00:00:00Z
P5875
P6179
1014078237