Enhanced bacterial protein expression during auto-induction obtained by alteration of lac repressor dosage and medium composition.
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Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complexStructure of Human J-type Co-chaperone HscB Reveals a Tetracysteine Metal-binding DomainX-ray structure ofDanio reriosecretagogin: A hexa-EF-hand calcium sensorStructural Insights into the Function of the Nicotinate Mononucleotide:phenol/ p -cresol Phosphoribosyltransferase (ArsAB) Enzyme from Sporomusa ovataFusion of Dioxygenase and Lignin-binding Domains in a Novel Secreted Enzyme from Cellulolytic Streptomyces sp. SirexAA-EBiochemical properties and atomic resolution structure of a proteolytically processed β-mannanase from cellulolytic Streptomyces sp. SirexAA-ESolution Structure of the 2A Protease from a Common Cold Agent, Human Rhinovirus C2, Strain W12Dual interaction of the Hsp70 J-protein cochaperone Zuotin with the 40S and 60S ribosomal subunitsChemical mechanism of UDP-galactopyranose mutase from Trypanosoma cruzi: a potential drug target against Chagas' diseaseA Protein Structure Initiative approach to expression, purification, and in situ delivery of human cytochrome b5 to membrane vesicles.The Center for Eukaryotic Structural Genomics.Active site and laminarin binding in glycoside hydrolase family 55High Yield of Recombinant Protein in Shaken E. coli Cultures with Enzymatic Glucose Release Medium EnPresso B.Soluble expression and purification of the oxidoreductase component of toluene 4-monooxygenase.Replication methods and tools in high-throughput cultivation processes - recognizing potential variations of growth and product formation by on-line monitoring.Collagen-like proteins in pathogenic E. coli strains.Recombinant protein expression in Escherichia coli: advances and challengesAuto-induction medium containing glyphosate for high-level incorporation of unusual aromatic amino acids into proteins.Contributions of unique active site residues of eukaryotic UDP-galactopyranose mutases to substrate recognition and active site dynamics.High-level expression of Bacillus naganoensis pullulanase from recombinant Escherichia coli with auto-induction: effect of lac operatorExpression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies.The Complex Energy Landscape of the Protein IscUMultifunctional cellulase catalysis targeted by fusion to different carbohydrate-binding modules.Batch production of a silk-elastin-like protein in E. coli BL21(DE3): key parameters for optimisation.Non-conventional induction strategies for production of subunit swine erysipelas vaccine antigen in rE. coli fed-batch cultures.Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulationSmall-scale, semi-automated purification of eukaryotic proteins for structure determination.Human mitochondrial chaperone (mtHSP70) and cysteine desulfurase (NFS1) bind preferentially to the disordered conformation, whereas co-chaperone (HSC20) binds to the structured conformation of the iron-sulfur cluster scaffold protein (ISCU).A putative siderophore-interacting protein from the marine bacterium Shewanella frigidimarina NCIMB 400: cloning, expression, purification, crystallization and X-ray diffraction analysisDetermination of glycoside hydrolase specificities during hydrolysis of plant cell walls using glycome profiling.Tuning different expression parameters to achieve soluble recombinant proteins in E. coli: advantages of high-throughput screening.Expression of a collagen-binding domain fusion protein: effect of amino acid supplementation, inducer type, and culture conditions.Structure-guided analysis of catalytic specificity of the abundantly secreted chitosanase SACTE_5457 from Streptomyces sp. SirexAA-E.Optimization of the Expression of DT386-BR2 Fusion Protein in Escherichia coli using Response Surface MethodologyEngineering the heterologous expression of lanthipeptides in Escherichia coli by multigene assembly.Yield Optimisation of Hepatitis B Virus Core Particles in E. coli Expression System for Drug Delivery Applications.A particular silent codon exchange in a recombinant gene greatly influences host cell metabolic activity.Autoinduction of protein expression.Phenotyping the quality of complex medium components by simple online-monitored shake flask experiments.Parallel use of shake flask and microtiter plate online measuring devices (RAMOS and BioLector) reduces the number of experiments in laboratory-scale stirred tank bioreactors.
P2860
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P2860
Enhanced bacterial protein expression during auto-induction obtained by alteration of lac repressor dosage and medium composition.
description
2007 nî lūn-bûn
@nan
2007年の論文
@ja
2007年論文
@yue
2007年論文
@zh-hant
2007年論文
@zh-hk
2007年論文
@zh-mo
2007年論文
@zh-tw
2007年论文
@wuu
2007年论文
@zh
2007年论文
@zh-cn
name
Enhanced bacterial protein exp ...... dosage and medium composition.
@en
type
label
Enhanced bacterial protein exp ...... dosage and medium composition.
@en
prefLabel
Enhanced bacterial protein exp ...... dosage and medium composition.
@en
P2093
P2860
P356
P1476
Enhanced bacterial protein exp ...... dosage and medium composition.
@en
P2093
Brian G Fox
Katie J Becker
Paul G Blommel
Petar Duvnjak
P2860
P304
P356
10.1021/BP070011X
P577
2007-05-17T00:00:00Z